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Investigation of diagnostic techniques for identification of infection S. stercoratis in patients with different forms of immunosuppression

Grant number: 10/51110-2
Support type:Regular Research Grants
Duration: September 01, 2010 - February 28, 2013
Field of knowledge:Biological Sciences - Parasitology
Principal Investigator:Pedro Paulo Chieffi
Grantee:Pedro Paulo Chieffi
Home Institution: Instituto de Medicina Tropical de São Paulo (IMT). Universidade de São Paulo (USP). São Paulo , SP, Brazil

Abstract

Infection with S. stercoralis is potentially severe in immunosuppressed patients, especially those who use steroids. Stool traditional techniques have low sensitivity. Thus, the availability of sensitive and specific diagnostic techniques is desirable, especially in the context of immunodeficiencies, where,the identification and treatment of helminthiasis are mandatory. Therefore, it is proposed to implement and validate the techniques of culture agar plate, the indirect immunofluorescence test, the ELISA and the Western blot for the diagnosis of strongyloidiasis in patients with different forms of immunosuppression, in addition to the identification of antigens from filariform larvae of S. venezuelensis viable for the standardization of serological diagnosis. Finally we will promote the standardization of the extraction of DNA for molecular diagnosis of this helminthiasis using the PCR technique. (AU)

Scientific publications (9)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
CORRAL, MARCELO ANDREETTA; DE PAULA, FABIANA MARTINS; MEISEL, DIRCE MARY C. L.; ABDALA, EDSON; COSTA, SILVIA FIGUEIREDO; PIERROTTI, LIGIA CAMERA; YAMASHIRO, JULIANA; DO NASCIMENTO GONCALVES, ELENICE M.; CASTILHO, VERA LUCIA P.; CHIEFFI, PEDRO PAULO; GRYSCHEK, RONALD CESAR B. IgG reactivity with 40-35 kDa soluble and membrane antigen of Strongyloides venezuelensis in immunocompromised patients. Acta Tropica, v. 190, p. 357-360, FEB 2019. Web of Science Citations: 0.
TOLEDO, BEATRIZ; CORRAL, MARCELO A.; MEISEL, DIRCE MARY C. L.; GOTTARDI, MAIARA; ABDALA, EDSON; COSTA, SILVIA F.; PIERROTTI, LIGIA CAMERA; LESCANO, SUSANA A. Z.; GONCALVES, ELENICE M. N.; CASTILHO, VERA L. P.; CHIEFFI, PEDRO P.; GRYSCHEK, RONALDO C. B.; PAULA, FABIANA M. Screening of Strongyloides infection using an ELISA test in transplant candidates. Clinics, v. 74, 2019. Web of Science Citations: 0.
PAULA, FABIANA M.; MALTA, FERNANDA M.; MARQUES, PRISCILLA D.; MELO, GESSICA B.; CORRAL, MARCELO A.; GOTTARDI, MAIARA; PINHO, JOAO R. R.; GONCALVES, ELENICE M. N.; CASTILHO, VERA L. P.; PIERROTTI, LIGIA C.; ABDALA, EDSON; COSTA, SILVIA F.; CHIEFFI, PEDRO P.; GRYSCHEK, RONALDO C. B. Molecular diagnosis of Strongyloides stercoralis among transplant candidates. TRANSPLANT INFECTIOUS DISEASE, v. 20, n. 4 AUG 2018. Web of Science Citations: 0.
DE PAULA, FABIANA MARTINS; MALTA, FERNANDA MELLO; CORRAL, MARCELO ANDREETTA; MARQUES, PRISCILLA DUARTE; GOTTARDI, MAIARA; CORREIA LIMA MEISEL, DIRCE MARY; YAMASHIRO, JULIANA; REBELLO PINHO, JOAO RENATO; PAGLIUSI CASTILHO, VERA LUCIA; DO NASCIMENTO GONCALVES, ELENICE MESSIAS; BORGES GRYSCHEK, RONALDO CESAR; CHIEFFI, PEDRO PAULO. DIAGNOSIS OF Strongyloides stercoralis INFECTION IN IMMUNOCOMPROMISED PATIENTS BY SEROLOGICAL AND MOLECULAR METHODS. Revista do Instituto de Medicina Tropical de São Paulo, v. 58, 2016. Web of Science Citations: 7.
GOTTARDI, MAIARA; PAULA, FABIANA M.; CORRAL, MARCELO A.; MEISEL, DIRCE MARY C.; COSTA, SILVIA F.; ABDALA, EDSON; PIERROTTI, LIGIA C.; YAMASHIRO, JULIANA; CHIEFFI, PEDRO PAULO; GRYSCHEK, RONALDO CESAR B. Immunofluorescence assay for diagnosis of strongyloidiasis in immunocompromised patients. INFECTIOUS DISEASES, v. 47, n. 8, p. 550-554, AUG 2015. Web of Science Citations: 1.
DE PAULA, FABIANA MARTINS; MALTA, FERNANDA DE MELLO; MARQUES, PRISCILLA DUARTE; SITTA, RENATA BARNABE; REBELLO PINHO, JOAO RENATO; BORGES GRYSCHEK, RONALDO CESAR; CHIEFFI, PEDRO PAULO. Molecular diagnosis of strongyloidiasis in tropical areas: a comparison of conventional and real-time polymerase chain reaction with parasitological methods. Memórias do Instituto Oswaldo Cruz, v. 110, n. 2, p. 272-274, APR 2015. Web of Science Citations: 10.
SITTA, R. B.; MALTA, F. M.; PINHO, J. R.; CHIEFFI, P. P.; GRYSCHEK, R. C. B.; PAULA, F. M. Conventional PCR for molecular diagnosis of human strongyloidiasis. Parasitology, v. 141, n. 5, p. 716-721, APR 2014. Web of Science Citations: 15.
PAULA, FABIANA MARTINS; SITTA, RENATA BARNABE; MALTA, FERNANDA MELLO; GOTTARDI, MAIARA; CORRAL, MARCELO ANDREETTA; BORGES GRYSCHEK, RONALDO CESAR; CHIEFFI, PEDRO PAULO. PARASITOLOGICAL AND MOLECULAR DIAGNOSIS IN EXPERIMENTAL Strongyloides venezuelensis INFECTION. Revista do Instituto de Medicina Tropical de São Paulo, v. 55, n. 2, p. 141-143, MAR-APR 2013. Web of Science Citations: 4.
FABIANA MARTINS PAULA; RENATA BARNABÉ SITTA; FERNANDA MELLO MALTA; MAIARA GOTTARDI; MARCELO ANDREETTA CORRAL; RONALDO CÉSAR BORGES GRYSCHEK; PEDRO PAULO CHIEFFI. PARASITOLOGICAL AND MOLECULAR DIAGNOSIS IN EXPERIMENTAL Strongyloides venezuelensis INFECTION. Revista do Instituto de Medicina Tropical de São Paulo, v. 55, n. 2, p. -, Abr. 2013.

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