Xenogenous collagen matrix graft with or without enamel matrix proteins derivative...
| Grant number: | 10/12777-1 |
| Support type: | Regular Research Grants |
| Duration: | December 01, 2010 - August 31, 2013 |
| Field of knowledge: | Health Sciences - Dentistry |
| Principal Investigator: | Arthur Belem Novaes Junior |
| Grantee: | Arthur Belem Novaes Junior |
| Home Institution: | Faculdade de Odontologia de Ribeirão Preto (FORP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil |
Abstract
Much attention is given to the amount of keratinized tissue (height and width) present around teeth and implants, being considered as an important factor in maintaining periodontal health and gingival esthetic. Researches had shown that when subgingival prostheses are inserted and a poor biofilm control is performed, increase of the incidence of gingival inflammation will occur, especially in thin keratinized mucosa, developing gingival recessions in teeth and implants. Thus in an attempt to increase the height and / or thickness of the keratinized mucosa, the autogenous subepithelial connective tissue graft has been indicated. In an attempt to avoid the need for an autogenous donor area, aiming to reduce morbidity and increase the speed of this procedure, recently, new products were developed, such as a collagen matrix of porcine origin (MCS). Therefore, this proposed work will assess whether this new MCS can increase the thickness of the keratinized gingival tissue and may prevent inflammation and formation of gingival recessions in teeth treated with prostheses with subgingival margins. Eight dogs Will be used, which will be conducted four experimental groups. Group 1 will receive the MCS in the region of P2, P3 and P4, with installation of prostheses. Group 2 will receive treatment with split flap without inclusion of MCS in the region of P2, P3 and P4, with installation of prostheses. The control group 1 will receive insertion of MCS in the region of P1, without installation of prostheses.The control group 2 will receive spli flap without insertion of prosthesis or the MCS in the region of P1. All prostheses used are inserted 8 weeks after surgery, with a 1mm subgingival margin. Twelve weeks after prostheses placement, the dogs will be euthanized. Clinical measures will be taken preoperatively, before prosthesis installation and before sacrifice. Will assess the presence or absence of gingival recessions and thickness of keratinized tissue. After samples preparation, the histomorphometric and immunohistochemistry analysis will be performed by evaluation of the height of the gingival margin and the area of tissue inflammation (inflammatory infiltrate, presence of lymphocytes and macrophages). Values are calculated as percentages and after the implementation of normality tests will be carried out statistical analysis parametric or nonparametric indicated. (AU)