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Evaluation of biofilm formed in situ, oral microorganisms and enamel demineralization after the use of probiotic-containing fermented milk


An increasing number of products containing probiotics, such as fermented milk, is available on the market to be used by consumers. Thus, the objective of this study will be to investigate the cariogenicity of probiotic-containing fermented milk through the quantification of total and reducer sugars in the product, analysis of its effect on demineralization of bovine dental enamel, microbiological analysis of saliva before and after the experimental period, microbiological analysis and quantification of alkali-soluble carbohydrates in the biofilm formed in situ, and microbiological evaluation of saliva after consumption of probiotic-containing fermented milk. It will be selected two brands of fermented milk for the in situ and in vivo study. Bovine enamel blocks will be selected by their microhardness surface for the in situ study and then fixed in acrylic palatal devices. Ten volunteers (four blocks per volunteer) will be included in this study. The in situ and in vivo experiment will be consisted of 3 and 2 phases of 14 days each with an interval of 7 days between the phases respectively. In the in situ experiment, the volunteers will drop a 20% sucrose solution or treatment solution (fermented milk brand A, fermented milk brand B) 8x/day. In the in vivo experiment, volunteers will drink the containing of a bottle of fermented milk/day. After the in situ experimental period, the biofilm will be analyzed for the amount of total microorganisms, total Streptococcus, Streptococcus mutans, Lactobacillus and alkali-soluble carbohydrates. In vivo study will be conducted with a initial collection of saliva and after 14 days to assess the total microrganisms, total Streptococcus, Streptococcus mutans and Lactobacillus. In the enamel blocks, it will be conducted the analysis of surface microhardness, percentage of surface microhardness change and microhardness in longitudinal section. (AU)