In vitro and in situ remineralization of approximal lesions in contact with different dental materials in primary teeth

• Grant number: 10/16120-7
• Support Opportunities: Regular Research Grants
• Duration: June 01, 2011 - May 31, 2013
• Field of knowledge: Health Sciences - Dentistry - Pediatric Dentistry
• Principal Investigator: Daniela Prócida Raggio
• Grantee: Daniela Prócida Raggio
• Host Institution: Faculdade de Odontologia (FO). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• Associated researchers: Camila de Almeida Brandão Guglielmi

Abstract

The aim of this study is to investigate in vitro (I) and in situ (II) the possible remineralization of carious lesions when in contact with proximal restorative materials that release fluoride, simulating contact with proximal restorations. For the study (I), 30 second molars will be sectioned in a bucco-lingual direction and each hemisection will be submitted to the chemical process of artificial caries induction by pH cycling. Measurements of hardness, light-induced fluorescence (QLF, Inspektor) and fluorescence camera (Vista Proof, Dürr Dental) will be performed at baseline and after the formation of lesions. Incisal-proximal cavities will be held on 30 first primary molars sectioned in bucco-lingual direction and restored with 6 different materials (n=10): two glass resin-modified ionomer cements (GIC) (Ketac N 100 and Vitremer - 3M ESPE), two high viscous GIC (Fuji IX - GC Corp. and Maxxion - FGM), polyacid-modified resin composite (RC) (Dyract eXtra - Dentsply) and conventional RC (Z 350 - 3M) as the control group. Each fragment with artificial caries lesion will be attached to a restored tooth, simulating the contact point. These sets will then be submitted to a new process of de-remineralization by 14 days and further evaluation of hardness, QLF camera and fluorescence will be held after 7 and 14 days for evaluation of remineralization. For the study (II), the specimens will be prepared in a similar manner and inserted in the intra-oral devices. Twelve volunteers will use the devices for 28 days, dripping 20%sucrose solution eight times a day. New analysis of microhardness with the QLF and fluorescence camera will be held after 14 and 28 days of use of intra-oral devices, similarly to the study (I). (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

BRANDAO GUGLIELMI, CAMILA DE ALMEIDA; BISSOTO CALVO, ANA FLAVIA; TEDESCO, TAMARA KERBER; MENDES, FAUSTO MEDEIROS; RAGGIO, DANIELA PROCIDA. Contact with Fluoride-Releasing Restorative Materials Can Arrest Simulated Approximal Caries Lesion. Journal of Nanomaterials, 2015. (10/16120-7)