Thermotolerance in cows: cellular and physiological approaches

• Grant number: 11/09282-3
• Support Opportunities: Regular Research Grants
• Start date: October 01, 2011
• End date: September 30, 2013
• Field of knowledge: Agronomical Sciences - Animal Husbandry - Ecology of Domestic Animals and Ethology
• Principal Investigator: Evaldo Antonio Lencioni Titto
• Grantee: Evaldo Antonio Lencioni Titto
• Host Institution: Faculdade de Zootecnia e Engenharia de Alimentos (FZEA). Universidade de São Paulo (USP). Pirassununga , SP, Brazil

Abstract

This study aims to quantify the expression of HSP70 in lymphocytes and oocytes correlating with heat stores heat of cows of different origins, submitted to artificial insemination at fixed time. In grazing systems, the physiological and behavioral responses triggered by high temperatures in summer, determine effects on conception rate, so is necessary to study the underlying mechanisms and identify viable solutions for increase productivity. The experimental phase will be developed in Terceira Island and Alentejo (Portugal) and São Paulo (Brazil) with Holstein Frisian and Limousine breeds. Animals of the breeds Mertolenga and Brahman will be also evaluated in Alentejo and São Paulo, respectively. In each experimental place will be undertaken four steps to which the animals are subjected. In the first step will be performed thermolysis capacity test, which aims to assess the relationship between the physiological and behavioral parameters and physiological thermotolerance. Simultaneously, the blood samples will be collected in order to undertake an assessment of gene expression of HSP70 in lymphocytes (second step). In the third step the female will undergo estrus synchronization so that artificial insemination is performed at fixed time. 28 days after artificial insemination will take place the pregnancy diagnosis by ultrasonography. As a fourth step will be harvested oocytes from females of various breeds in order to study to evaluate the expression of HSP70. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

HOOPER, HENRIQUE BARBOSA; TITTO, CRISTIANE GONCALVES; GONELLA-DIAZA, ANGELA MARIA; HENRIQUE, FABIO LUIS; PULIDO-RODRIGUEZ, LINA FERNANDA; SILVA LONGO, ANA LUISA; DA CUNHA LEME-DOS-SANTOS, THAYS MAYRA; PEREIRA DE MIRA GERALDO, ANA CARINA ALVES; FRANCO PEREIRA, ALFREDO MANUEL; BINELLI, MARIO; et al. Heat loss efficiency and HSPs gene expression of Nellore cows in tropical climate conditions. INTERNATIONAL JOURNAL OF BIOMETEOROLOGY, v. 63, n. 11, SI, p. 1475-1486, NOV 2019. (11/09282-3)