Development of new mass spectrometry techniques and their general applications in science: chemistry, biochemistry, material sciences, forensics, medicine, food science, pharmaceutical and veterinary medicine

• Grant number: 10/51677-2
• Support Opportunities: Research Projects - Thematic Grants
• Start date: November 01, 2011
• End date: December 31, 2016
• Field of knowledge: Physical Sciences and Mathematics - Chemistry
• Principal Investigator: Marcos Nogueira Eberlin
• Grantee: Marcos Nogueira Eberlin
• Host Institution: Instituto de Química (IQ). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil
• Associated scholarship(s): 13/20313-3 - Identification of the substances present in red Brazilian propolis by UPLC-ESI-MS and NMR and evaluation of the antioxidant and antimicrobial activity of the isolated compounds, BP.DR; 13/23920-8 - Development of new mass spectrometry techniques and their general applications in science: chemistry, biochemistry, material sciences, forensics, medicine, food science, pharmaceutical and veterinary medicine, BP.PD; 12/17032-0 - Mechanistic studies by mass spectrometry of the Knoevenagel condensation using functionalized ionic liquids, BP.PD; + associated scholarships 12/20843-0 - The role of exogenous melatonin on in vitro maturation medium for bovine and porcine oocytes: determination and quantification of the main metabolites by mass spectrometry., BP.PD; 12/21395-0 - Molecular characterization of sedimentary organic matter in the Estuary-Lagoon System Cananéia-Iguape by Fourier transform ion cyclotron resonance mass spectrometry, BP.DR; 12/07206-0 - Search for a Molecular Signature of Receptivity in Cattle by Mass Spectrometry, BP.PD; 12/03091-4 - Analysis of the composition of samples of red Brazilian propolis by mass spectrometry with electrospray ionization and ultra high efficiency liquid chromatography (UPLC-ESI-MS) and evaluation of the antioxidant and antimicrobial activity., BP.MS - associated scholarships

Abstract

Since its infancy, mass spectrometry (MS) has been directly involved in major scientific developments. From the discovery of the electron and the isotopes, and nuclear energy, to the recent and extraordinary developments in protein characterization, the MS technique has provided Science with a very diverse and useful set of tools. During the last two decades, with the development of revolutionary ionization techniques and new instruments, the MS technique has experienced even more intense and extraordinary growth, being today an indispensable technique in various fields in Science. The ThoMSon Mass Spectrometry Laboratory at the Institute of Chemistry _ University of Campinas - UNICAMP, has been since 1992 very active in the development of new MS techniques and new MS applications, and is placed today among the most active and productive MS research groups worldwide. The ThoMSon lab has been very active, with great emphasis via multidisciplinary collaborations with national and International groups, in the formation of a new generation of Brazilian experts in MS. This thematic project has therefore the objective to consolidate and expand the leading role of the ThoMSon lab in the development of new MS techniques and applications. The project also opens new research areas such as those related to studies on hormones, forensic science and bacterial screening. A variety of research proposals are described in diverse areas: applications of ambient mass spectrometry (EASI-MS) in chemistry and forensic chemistry; characterization of coordination and supramolecular organo-mettalic species via ESI-MS/MS; ion/molecule reaction studies via pentaquadrupole mass spectrometry; monitoring of the destruction of pollutants in solution via ESI-MS/MS and V-EASI-MS; differentiation of constitutional isomers and enantiomers via TWIM-MS; fringerprinting of complex chemical mixtures via ESI-MS/MS; new applications of via LC-MS/MS techniques in reproduction and veterinary medicine; and microorganism identification via MALDI-MS. It is expected, therefore, that this thematic project will result in even more intense and higher quality scientific production, and in the more intense formation of highly qualified researchers and professionals in an area which is presently of great relevance and demand in Science and Technology. (AU)

Scientific publications

(9)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

CÉLIO F. F. ANGOLINI; ANA B. GONÇALVES; RENATA SIGRIST; BRUNO S. PAULO; MARKIYAN SAMBORSKYY; PEDRO L. R. CRUZ; ADRIANA F. VIVIAN; EDUARDO M. SCHMIDT; MARCOS N. EBERLIN; WELINGTON L. ARAÚJO; et al. Genome Mining of Endophytic Streptomyces wadayamensis Reveals High Antibiotic Production Capability. Journal of the Brazilian Chemical Society, v. 27, n. 8, p. 1465-1475, 2016-08-00. (15/01013-4, 14/12727-5, 10/51677-2, 13/12598-8)

FERNANDES, ANNA MARIA A. P.; VENDRAMINI, PEDRO H.; GALAVERNA, RENAN; SCHWAB, NICOLAS V.; ALBERICI, LUCIANE C.; AUGUSTI, RODINEI; CASTILHO, ROGER F.; EBERLIN, MARCOS N.. Direct Visualization of Neurotransmitters in Rat Brain Slices by Desorption Electrospray Ionization Mass Spectrometry Imaging (DESI - MS). JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY, v. 27, n. 12, p. 1944-1951, DEC 2016. (10/51677-2, 11/50400-0)

MARTINS-JUNIOR, HELIO A.; PINAFFI, FABIO L. V.; SIMAS, ROSINEIDE C.; TAROUCO, ADRIANA K.; FERREIRA, CHRISTINA R.; SILVA, LUCIANO A.; NOGUEIRA, GUILHERME P.; MEIRELLES, FLAVIO V.; EBERLIN, MARCOS N.; PERECIN, FELIPE. Plasma Steroid Dynamics in Late- and Near-term Naturally and Artificially Conceived Bovine Pregnancies as Elucidated by Multihormone High-resolution LC-MS/MS. Endocrinology, v. 155, n. 12, p. 5011-5023, DEC 2014. (10/05806-5, 10/15167-0, 10/51677-2)

PAULO, BRUNO S.; SIGRIST, RENATA; ANGOLINI, CELIO F. F.; EBERLIN, MARCOS N.; DE OLIVEIRA, LUCIANA G.. Gene Deletion Leads to Improved Valinomycin Production by Streptomyces sp. CBMAI 2042. Journal of the Brazilian Chemical Society, v. 30, n. 3, SI, p. 673-679, MAR 2019. (14/50249-8, 15/01013-4, 10/51677-2, 14/12727-5, 13/12598-8)

NEGRAO, FERNANDA; ABANADES, DANIEL R.; JAEEGER, CAROLINE F.; ROCHA, DANIELE F. O.; BELAZ, KATIA R. A.; GIORGIO, SELMA; EBERLIN, MARCOS N.; ANGOLINI, CELIO F. F.. Lipidomic alterations of in vitro macrophage infection by L-infantum and L-amazonensis. MOLECULAR BIOSYSTEMS, v. 13, n. 11, p. 2401-2406, NOV 2017. (16/11517-2, 15/23767-0, 12/07206-0, 13/11100-6, 10/51677-2)

ANGOLINI, CELIO FERNANDO F.; VENDRAMINI, PEDRO HENRIQUE; ARAUJO, FRANCISCA D. S.; ARAUJO, WELINGTON L.; AUGUSTI, RODINEI; EBERLIN, MARCOS N.; DE OLIVEIRA, LUCIANA GONZAGA. Direct Protocol for Ambient Mass Spectrometry Imaging on Agar Culture. Analytical Chemistry, v. 87, n. 13, p. 6925-6930, JUL 7 2015. (10/51677-2)

COELHO, MIRELA B.; RODRIGUES-CUNHA, MARIA CAROLINA; FERREIRA, CHRISTINA R.; CABRAL, ELAINE C.; NOGUEIRA, GUILHERME P.; EBERLIN, MARCOS N.; LEAL, CLAUDIA L. V.; SIMAS, ROSINEIDE C.. Assessing melatonin and its oxidative metabolites amounts in biological fluid and culture medium by liquid chromatography electrospray ionization tandem mass spectrometry (LC-ESI-MS/MS). ANALYTICAL METHODS, v. 5, n. 24, p. 6911-6918, 2013. (10/51677-2, 12/20843-0, 10/18023-9)

GOBBO FERRARI, SARITA P.; BONASSA, KARINA P. D.; COELHO, MIRELA B.; FERREIRA, CHRISTINA R.; DA COSTA, HENI FALCAO; JARA, JOSE LUIS P.; MIGUEL, MARIA CAROLINA V.; REYES, FELIX GUILLERMO R.; EBERLIN, MARCOS N.; NOGUEIRA, GUILHERME P.; et al. High precision and selectivity for quantitation of enrofloxacin and ciprofloxacin in five chicken tissues using solid phase extraction and ESI LC-MS/MS for application in monitoring residues. ANALYTICAL METHODS, v. 7, n. 7, p. 3291-3297, 2015. (10/51677-2, 10/15167-0)

MARTINS-JUNIOR, HELIO A.; SIMAS, ROSINEIDE C.; BROLIO, MARINA P.; FERREIRA, CHRISTINA R.; PERECIN, FELIPE; NOGUEIRA, GUILHERME DE P.; MIGLINO, MARIA A.; MARTINS, DANIELE S.; EBERLIN, MARCOS N.; AMBROSIO, CARLOS E.. Profiles of Steroid Hormones in Canine X-Linked Muscular Dystrophy via Stable Isotope Dilution LC-MS/MS. PLoS One, v. 10, n. 5, MAY 26 2015. (10/51677-2, 07/51222-2, 10/15167-0)