Comparison of anti-inflammatory and antioxidant effects of different intra-articular therapies in osteochondrotic horses

• Grant number: 11/19096-2
• Support Opportunities: Regular Research Grants
• Start date: December 01, 2011
• End date: May 31, 2014
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Animal Clinics and Surgery
• Principal Investigator: Raquel Yvonne Arantes Baccarin
• Grantee: Raquel Yvonne Arantes Baccarin
• Host Institution: Faculdade de Medicina Veterinária e Zootecnia (FMVZ). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• Associated researchers: Cristina de Oliveira Massoco Salles Gomes ; Luis Cláudio Lopes Correia da Silva ; Yara Maria Corrêa da Silva Michelacci

Abstract

Articular diseases are listed among the most common and important affections in the equine athlete, being the disturbance of articular homeostasis and inefficacy of healing processes crucial factors in the fate of the cartilagineous lesion. The use of natural antagonists or analogous synthetic products to refrain the degenerative process from progressing, as well as to promote joint homeostasis, has received increasing attention not only in equine medicine, but in other domestic animals and human beings. This study aims to compare different therapeutic modalities in respect to their anti-inflammatory and antioxidant properties, including platelet rich plasma (PRP), interleukin-1 receptor antagonist protein (IRAP II®), autologous plasma (AP), hyaluronic acid (HA), and the association between hyaluronic acid and autologous plasma (HA+ AP) in synovial fluid cells stimulated in vitro by the addition of LPS and to verify their effect in the reestablishment of articular homeostasis in osteochondritic joints in vivo. In the initial part of the experiment synovial fluid of 12 healthy tarsocrural joints will be collected in two phases. In the first phase (anti-inflammatory evaluation) the synovial cell pool will be cultivated and subjected to different treatments. The supernatant will be collected 24 and 48 hours after the addition of LPS to determine prostaglandin E2 (PGE2), interleukin-1 (IL-1), interleukin-1 receptor antagonist (IL-1ra) and tumor necrosis factor ± (TNF±). In the second phase the antioxidant effects of different therapeutic protocols will be evaluated on synovial fluid cells challenged by LPS, through the determination of their oxidative burst. In a final step, the anti-inflammatory effects of the treatments will be evaluated in vivo, in 36 osteochondritic tarsocrural joints, that will be randomly assigned to 6 groups, according to the intra articular therapy received. Synovial fluid will be collected before arthroscopic surgery and 48 hours after the surgical procedure. Those samples will be evaluated regarding their physical characteristics, mucin precipitation clot, total and differential white cell count, total protein content determination, hyaluronic acid and chondroitin sulphate measurement and quantification of PGE2, TNF-a, IL-1 and IL- 1ra. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

MACHADO, THAIS S. L.; MASSOCO, CRISTINA O.; SILVA, LUIS CLAUDIO L. C.; FULBER, JOICE; MOREIRA, JULIANA J.; BACCARIN, RAQUEL Y. A.. Effects of blood-derived products and sodium hyaluronate on equine synovial fluid cells and on synovial fluid from osteochondrotic joints of horses after arthroscopy and administration of treatment. AMERICAN JOURNAL OF VETERINARY RESEARCH, v. 80, n. 7, p. 646-656, JUL 2019. (11/19096-2, 11/12929-9)