Influence of peracetic acid and sodium hypochorite in the cleaning of root canals instrumented with different rotary systems

• Grant number: 11/22022-0
• Support Opportunities: Regular Research Grants
• Start date: May 01, 2012
• End date: April 30, 2013
• Field of knowledge: Health Sciences - Dentistry - Endodontics
• Principal Investigator: Ana Paula Martins Gomes
• Grantee: Ana Paula Martins Gomes
• Host Institution: Instituto de Ciência e Tecnologia (ICT). Universidade Estadual Paulista (UNESP). Campus de São José dos Campos. São José dos Campos , SP, Brazil

Abstract

The objective of this study will be evaluated, by scanning electron microscopy analysis, the effect of peracetic acid solution 0,5% and 2% and sodium hypochlorite solution 5,25% in the root canal walls after rotary instrumentation. Will be used 80 single-rooted human roots with standardized length of 16 mm and will have their canals instrumented by two rotary systems (Biorace and Mtwo), varying the irrigation solution, as follows (n=10): G1H - Instrumentation with Biorace System + sodium hypochlorite solution 5.25%; G2H - Instrumentation with Mtwo System + -sodium hypochlorite solution 5.25%; G3A - Instrumentation with Biorace System + peracetic acid solution 0.5%; G4A - Instrumentation with Mtwo System + peracetic acid solution 0.5%; G5A - Instrumentatio with Biorace System + peracetic acid solution 2%; G6A - Instrumentation with Mtwo System + peracetic acid solution 2%; G7S - Instrumentation with Biorace System + saline solution (control); G8S - Instrumentation with Mtwo System + saline solution (control). After instrumentation, the roots will be cleaved and the dentin walls will be evaluated by SEM with 500x and 2000x magnification for evaluation the cleaning of the root canals at the cervical, middle and apical thirds. The analyzed area will be quantified according by the total number of tubules present and the percentage of open tubules at each region. Data will be submitted to statistical analysis by ANOVA and Tukey tests, at the significance level of 5%. (AU)