Effectiveness of experimental light-cured varnishes and tooth brushing in reducing the biofilm adhesion and biofilms formation to a denture base acrylic resin

• Grant number: 12/01528-6
• Support Opportunities: Regular Research Grants
• Start date: August 01, 2012
• End date: July 31, 2014
• Field of knowledge: Health Sciences - Dentistry - Dental Materials
• Principal Investigator: Eunice Teresinha Giampaolo
• Grantee: Eunice Teresinha Giampaolo
• Host Institution: Faculdade de Odontologia (FOAr). Universidade Estadual Paulista (UNESP). Campus de Araraquara. Araraquara , SP, Brazil

Abstract

Considering that the biofilm adhesion on the surface of the partial or complete dentures is a prerequisite for colonization and, consequently, for denture stomatitis, the development of methods that minimize or prevent the adhesion to the acrylic resin could produce significant advance in prevention of disease. An alternative treatment would be the surface modification of materials for denture base. Thus, the aim of this study is to evaluate two experimental varnishes in three different concentrations as a method to modify the surface of a smooth and rough acrylic resin, in order to verify (i) the ability of coatings to prevent adhesion and the multi-species biofilm formation on smooth and rough acrylic resin surfaces, with or without prior exposure to human saliva, (ii) the effectiveness of mechanical brushing on the removal of the biofilm formed on the surface modified, (iii) the permanence of varnish over time, when subjected to mechanical brushing . Specimens with 10 mm diameter and 2 mm thick are made with surface features that mimic the polished part of the prosthesis (between glass) and surface features that mimic the inside of the prosthesis (between rough glass) using a microwave cured resin. After that, the specimens will be randomly divided into 6 experimental groups, according to the varnish used and its concentration, and a control group that received no varnish on its surface. The adhesion of biofilm on the denture base resin will be measured by XTT assay and by colony count (cfu). To evaluate the removal capacity of biofilm, the specimens will be subjected to toothbrushing test and then will analyze the biofilm remaining. Finally, the coating longevity will be evaluated qualitatively through a stereoscopic microscope and quantitatively, by spectroscopic analysis with X-ray photoelectron (XPS) in different periods of time. The results will be statistically analyzed for further interpretation and discussion. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

IZUMIDA, FERNANDA EMIKO; MOFFA, EDUARDO BUOZI; VERGANI, CARLOS EDUARDO; MACHADO, ANA LUCIA; JORGE, JANAINA HABIB; GIAMPAOLO, EUNICE TERESINHA. In vitro evaluation of adherence of Candida albicans, Candida glabrata, and Streptococcus mutans to an acrylic resin modified by experimental coatings. BIOFOULING, v. 30, n. 5, p. 525-533, MAY 28 2014. (10/00545-9, 12/01528-6)

MOFFA, EDUARDO B.; MACHADO, MARIA A. A. M.; MUSSI, MARIA C. M.; XIAO, YIZHI; GARRIDO, SAULO S.; GIAMPAOLO, EUNICE T.; SIQUEIRA, WALTER L.. In Vitro Identification of Histatin 5 Salivary Complexes. PLoS One, v. 10, n. 11, NOV 6 2015. (12/01528-6, 11/23540-5, 11/23543-4, 13/15412-2)