Molecular investigation of Klebsiella pneumoniae isolates and plasmids carrying beta-lactamases encoding genes

• Grant number: 12/14740-3
• Support Opportunities: Regular Research Grants
• Start date: October 01, 2012
• End date: September 30, 2014
• Field of knowledge: Biological Sciences - Microbiology - Applied Microbiology
• Principal Investigator: Ana Lúcia da Costa Darini
• Grantee: Ana Lúcia da Costa Darini
• Host Institution: Faculdade de Ciências Farmacêuticas de Ribeirão Preto (FCFRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil

Abstract

Beta-lactamases -producing enterobacterias are globally spread mainly ESBL (CTX-M) and carbapenem resistant-enterobacterial isolates. The objective of this work was study the genetic and molecular epidemiology of ESBL and carbapenem resistant-Klebsiella isolates from patients of Northeast region of Sao Paulo State and the plasmids involved. Identification and antimicrobial susceptibility testing will be accomplished by using semiautomatic systems and standard methods. Clonal relatedness will established by Pulsed-Field Gel Electrophoresis (PFGE) and also by multilocus sequence typing. The presence of genes encoding carbapenemases and extended spectrum ²-lactamases and characterization of bla genes, genetic environment and plasmids will include PCR and further sequencing, RFLP analyses, S1-PFGE and hybridization. This work will point out the high diversity of available genetic elements harboring bla genes. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

ANDRADE, LEONARDO NEVES; NOVAIS, ANGELA; MARCATO STEGANI, LENITA MARIA; FERREIRA, JOSEANE CRISTINA; RODRIGUES, CARLA; COSTA DARINI, ANA LUCIA; PEIXE, LUISA. Virulence genes, capsular and plasmid types of multidrug-resistant CTXM(-2,-8,-15) and KPC-2-producing Klebsiella pneumoniae isolates from four major hospitals in Brazil. DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE, v. 91, n. 2, p. 164-168, JUN 2018. (12/14740-3, 11/08892-2, 13/26188-6)

ANDRADE, LEONARDO NEVES; VITALI, LUCIA; GASPAR, GILBERTO GAMBERO; BELLISSIMO-RODRIGUES, FERNANDO; MARTINEZ, ROBERTO; COSTA DARINI, ANA LUCIA. Expansion and Evolution of a Virulent, Extensively Drug-Resistant (Polymyxin B-Resistant), QnrS1-, CTX-M-2-, and KPC-2-Producing Klebsiella pneumoniae ST11 International High-Risk Clone. Journal of Clinical Microbiology, v. 52, n. 7, p. 2530-2535, JUL 2014. (12/14740-3, 11/08892-2)