Acute effect of exercise on molecular pathways related to skeletal muscle glucose uptake in patients with polycystic ovary syndrome

• Grant number: 12/14650-4
• Support Opportunities: Regular Research Grants
• Duration: October 01, 2012 - May 31, 2014
• Field of knowledge: Health Sciences - Physical Education
• Principal Investigator: Bruno Gualano
• Grantee: Bruno Gualano
• Host Institution: Escola de Educação Física e Esporte (EEFE). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• Associated researchers: Ana Lúcia de Sá Pinto ; José Antonio Miguel Marcondes ; Maria Elizabeth Rossi da Silva

Abstract

The polycystic ovary syndrome (PCOS) is a hormonal disorder characterized by chronic hyperandrogenic anovulation with reproductive, aesthetic, metabolic, and oncologic consequences, affecting approximately 5-10% of women during reproductive age. The hyperinsulinemia and the insulin resistance are features often seen in PCOS patients, irrespective of body mass, although such conditions are clearly more prevalent in obesity. A recent study demonstrated that an exercise training program led to improvements in insulin sensitivity in PCOS patients. However, exercise was unable to fully resolve the insulin resistance, suggesting that PCOS patients present a partial response to exercise stimulus. Therefore, novel investigations may elucidate the molecular mechanisms by which exercise stimulates (or fails in stimulating) the glucose uptake in PCOS. The comprehension of the mechanisms associated with insulin resistance in PCOS is of great relevance to the development of therapeutic strategies (i.e., drugs or exercise) aimed at treating this syndrome. In this context, the acute exercise emerges as an excellent research model, since chronic adaptations to exercise have been thought as the summation of exercise acute effects in each training session. The main objective of this project will be to investigate the acute effect of exercise on molecular pathways related to skeletal muscle glucose uptake in patients with polycystic ovary syndrome. The selected patients will undertake a maximal exercise test to determine parameters related to the acute exercise prescription. After one week, the patients will be submitted to a muscle biopsy at rest. Thereafter, they will perform a moderate-intensity exercise bout, and immediately after the exercise, a second muscle biopsy will be done. A BMI-, age-, and VO2max-matched healthy group will perform the same above mentioned protocol, serving as controls. The muscle samples will be analyzed for PI3-k, phospho-IR, phospho-IRS-1, phospho-IRS-2, phospho-AMPK, phospho-AKT and total and membrane GLUT-4. (AU)

Scientific publications

(6)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DANTAS, WAGNER SILVA; MURAI, IGOR HISASHI; PERANDINI, LUIZ AUGUSTO; AZEVEDO, HATYLAS; MOREIRA-FILHO, CARLOS ALBERTO; SARAIVA CAMARA, NIELS OLSEN; ROSCHEL, HAMILTON; GUALANO, BRUNO. Acute exercise elicits differential expression of insulin resistance genes in the skeletal muscle of patients with polycystic ovary syndrome. Clinical Endocrinology, v. 86, n. 5, p. 688-697, MAY 2017. (12/14650-4, 12/02827-7)

DANTAS, WAGNER SILVA; MURAI, IGOR HISASHI; PERANDINI, LUIZ AUGUSTO; AZEVEDO, HATYLAS; MOREIRA-FILHO, CARLOS ALBERTO; SARAIVA CAMARA, NIELS OLSEN; ROSCHEL, HAMILTON; GUALANO, BRUNO. Acute exercise elicits differential expression of insulin resistance genes in the skeletal muscle of patients with polycystic ovary syndrome. CLINICAL ENDOCRINOLOGY, v. 86, n. 5, p. 10-pg., 2017-05-01. (12/02827-7, 12/14650-4)

DANTAS, WAGNER SILVA; GUALANO, BRUNO; ROCHA, MICHELE PATROCINIO; GRIMALDI BARCELLOS, CRISTIANO ROBERTO; VIEIRA YANCE, VIVIANE DOS REIS; MIGUEL MARCONDES, JOSE ANTONIO. Metabolic Disturbance in PCOS: Clinical and Molecular Effects on Skeletal Muscle Tissue. SCIENTIFIC WORLD JOURNAL, 2013. (12/14650-4, 12/02827-7)

DANTAS, WAGNER SILVA; DAS NEVES, WILLIAN; GIL, SAULO; GRIMALDI BARCELLOS, CRISTIANO ROBERTO; ROCHA, MICHELE PATROCINIO; DE SA-PINTO, ANA LUCIA; ROSCHEL, HAMILTON; GUALANO, BRUNO. Exercise-induced anti-inflammatory effects in overweight/obese women with polycystic ovary syndrome. CYTOKINE, v. 120, p. 66-70, AUG 2019. (12/14650-4, 13/04744-4)

DANTAS, WAGNER SILVA; MIGUEL MARCONDES, JOSE ANTONIO; SHINJO, SAMUEL KATSUYUKI; PERANDINI, LUIZ AUGUSTO; ZAMBELLI, VANESSA OLZON; DAS NEVES, WILLIAN; GRIMALDI BARCELLOS, CRISTIANO ROBERTO; ROCHA, MICHELE PATROCINIO; VIEIRA YANCE, VIVIANE DOS REIS; DOS SANTOS PEREIRA, RENATO TAVARES; et al. GLUT4 translocation is not impaired after acute exercise in skeletal muscle of women with obesity and polycystic ovary syndrome. OBESITY, v. 23, n. 11, p. 2207-2215, NOV 2015. (12/14650-4, 12/02827-7)

DANTAS, WAGNER SILVA; GUALANO, BRUNO; ROCHA, MICHELE PATROCINIO; GRIMALDI BARCELLOS, CRISTIANO ROBERTO; VIEIRA YANCE, VIVIANE DOS REIS; MIGUEL MARCONDES, JOSE ANTONIO. Metabolic Disturbance in PCOS: Clinical and Molecular Effects on Skeletal Muscle Tissue. SCIENTIFIC WORLD JOURNAL, v. N/A, p. 7-pg., 2013-01-01. (12/02827-7, 12/14650-4)