Enzymatic reactions: exploiting your potential as synthetic tool

• Grant number: 12/14196-1
• Support Opportunities: Regular Research Grants
• Start date: November 01, 2012
• End date: October 31, 2014
• Field of knowledge: Physical Sciences and Mathematics - Chemistry - Organic Chemistry
• Principal Investigator: Luiz Fernando da Silva Júnior
• Grantee: Luiz Fernando da Silva Júnior
• Host Institution: Instituto de Química (IQ). Universidade de São Paulo (USP). São Paulo , SP, Brazil

Abstract

Biocatalysis has been established as a powerful tool in organic synthesis. The enzyme-catalyzed reactions mostly distinguished by their high chemo-, regio- and stereo-selectivity. In this project we will present different topics related to the use of enzymatic reactions in organic synthesis. We will study the application of enzymatic reactions involving organic compounds containing heteroatoms such as B, Si and Sn. In addition to the reactions catalyzed by commercial enzymes, we intend to pursue and characterize microbial alcohol dehydrogenases for application in enantioselective bioreduction of ketones and enantioselective oxidation of secondary alcohols. From other projects, we have isolated several bacteria, which the enzymatic activity was excellent (alcohol dehydrogenase), then in this project, we intend to carry out the bacterial genome sequencing. Finally, we intent to study how the activity of Burkholderia cepacia lipase and porcine pancreas immobilized on Ag-Au nanoparticles can be influenced by changing the particles surface with different organic compounds. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

PALMEIRA, DAYVSON J.; ARAUJO, LIDIANE S.; ABREU, JULIANA C.; ANDRADE, LEANDRO H.. Application of a promiscuous Arthrobacter sp from Antarctic in aerobic (R)-selective deracemization and anaerobic (S)-selective reduction. JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC, v. 110, p. 117-125, DEC 2014. (12/14196-1, 12/11482-3, 11/01376-9)

KISUKURI, CAMILA M.; MACEDO, ALEXANDRA; OLIVEIRA, CAIO C. S.; CAMARGO, PEDRO H. C.; ANDRADE, LEANDRO H.. Investigating the Influence of the Interface in Thiol-Functionalized Silver-Gold Nanoshells over Lipase Activity. Langmuir, v. 29, n. 51, p. 15974-15980, DEC 24 2013. (12/11201-4, 11/06847-0, 12/14196-1)

ANDRADE, LEANDRO H.; SOUSA, BRUNO A.; FERREIRA, IRLON M.; PORTO, ANDRE L. M.. Contributions on Kinetic Resolution by Lipases on the Development of Organic Synthesis in Brazil. CURRENT ORGANIC SYNTHESIS, v. 12, n. 6, p. 696-713, 2015. (12/14196-1)