Characterization on unconventional aqueous two-phase system and its application in bromelain purification from agroindustrial wastes

• Grant number: 12/14533-8
• Support Opportunities: Regular Research Grants
• Start date: November 01, 2012
• End date: October 31, 2014
• Field of knowledge: Engineering - Chemical Engineering
• Principal Investigator: Elias Basile Tambourgi
• Grantee: Elias Basile Tambourgi
• Host Institution: Faculdade de Engenharia Química (FEQ). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil
• City of the host institution: Campinas
• Associated researchers: Diego de Freitas Coêlho

Abstract

Bromelain is the generic name given to the group of endopeptidases coming from all members belonging to the Bromeliaceae family, which belongs to the pineapple (Ananas comosus) and curauá (Ananas erectifolius). Due to its important physiological roles, these enzymes provide a broad spectrum of proven therapeutic applications proven (anti-inflammatory, anti-tumor; adjuvant healing, increased cardiac capacity, etc.), being used by the food industry in meat processing. For industrial production of bromelain, should preferably use the waste generated by the processing of pineapple and curauá, since there is such a large amount of bromelain and the main products of the processes (e.g., fruit juice and pineapple, and fibers curauá) have a high commercial value. Thus, the study of the extraction of bromelain from these agroindustrial wastes using aqueous two-phase systems (ATPS's) may be an interesting alternative for their purification and production. This technique exploits the capacity displayed by solutions of two polymers or one polymer and a salt to separate into two aqueous phases when certain concentrations of these components are exceeded. These phases have high water content, and in some cases levels greater than 90%, providing an environment sufficient mild to preserve the stability of molecular biomolecules. Thus, studies that aim to purify the bromelain from waste with the objective of marketing for the pharmaceutical industry are highly promising in terms of science and technology. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

COELHO, DIEGO F.; SATURNINO, THAIS PERON; FERNANDES, FERNANDA FREITAS; MAZZOLA, PRISCILA GAVA; SILVEIRA, EDGAR; TAMBOURGI, ELIAS BASILE. Azocasein Substrate for Determination of Proteolytic Activity: Reexamining a Traditional Method Using Bromelain Samples. BIOMED RESEARCH INTERNATIONAL, 2016. (12/14533-8, 11/20733-7)

COELHO, DIEGO F.; SATURNINO, THAIS PERON; FERNANDES, FERNANDA FREITAS; MAZZOLA, PRISCILA GAVA; SILVEIRA, EDGAR; TAMBOURGI, ELIAS BASILE. Azocasein Substrate for Determination of Proteolytic Activity: Reexamining a Traditional Method Using Bromelain Samples. BIOMED RESEARCH INTERNATIONAL, v. 2016, p. 6-pg., 2016-01-01. (11/20733-7, 12/14533-8)