Effects of PDGF-BB on the rate of proliferation and on the adhesion of human cells derived from bone repair tissue to root fragments

• Grant number: 12/14044-7
• Support Opportunities: Regular Research Grants
• Start date: December 01, 2012
• End date: May 31, 2015
• Field of knowledge: Health Sciences - Dentistry
• Principal Investigator: Adriana Campos Passanezi Santana
• Grantee: Adriana Campos Passanezi Santana
• Host Institution: Faculdade de Odontologia de Bauru (FOB). Universidade de São Paulo (USP). Bauru , SP, Brazil

Abstract

The aim of this study is to investigate the role of platelet derived growth factor-BB (PDGF-BB) on the rate of proliferation and adhesion of human cells derived from bone repair tissue in primary culture to periodontally compromised root fragments. Four systemically healthy patients, both genders, requiring periodontal surgical treatment by newly forming bone graft (NFBG) technique will be selected. After 21 days of the creation of a surgical alveolus, a small biopsy will be collected from the medium third of the alveolus for primary culture. After the establishment and immunohistochemical characterization of the sample, the effects of recombinant human PDGF-BB at 300 ng/ml on the rate of proliferation and adhesion of cells derived from bone repair tissue will be investigate. The rate of cell proliferation stimulated by PDGF-BB (test group) and by culture medium (negative control group) will be investigated by counting the number of viable cells in tissue flasks 1, 3, 5 and 7 days after seeding. Afterwards, 15 teeth extracted from periodontal reasons will be collected. The teeth will be split into two halves according to tooth long axis, following ressection of crown and apical third of the root, resulting in 30 root fragments that will be divided into two groups: test (n=15)- addition of 300ng/ml of PDGF-BB to culture medium; negative control (n=15)- culture medium consisting of DMEM, 10%FBS and 1% antibiotic solution. All fragments will be scaled and root planned (20 strokes) with Gracey curettes followed by conditioning of root surfaces with a gel solution of 24% EDTA for 3 minutes and vigorous rinsing with saline solution. Fragments will be air dried, sterilized and positioned in 24-well plates. A total of 104 cells derived from bone repair tissue will be seeded on root fragments and incubated for 24 hours. The material will be fixated and prepared for analysis in scanning electron microscopy. Photomicrography will be evaluated by two blinded examiners, who will count the number of cells adhered to root surfaces. The results obtained will be investigated by parametric t test. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

PASSANEZI SANT'ANA, ADRIANA CAMPOS; DAMANTE, CARLA ANDREOTTI; FRIAS MARTINEZ, MARIA ALEJANDRA; MEDINA VALDIVIA, MARIA ALEJANDRA; HAGE KARAM, PAULA STEFINIA; DE OLIVEIRA, FLAVIA AMADEU; DE OLIVEIRA, RODRIGO CARDOSO; GASPAROTO, THAIS HELENA; CAMPANELLI, ANA PAULA; RAGGHIANTI ZANGRANDO, MARIANA SCHUTZER; et al. Isolation and characterization of progenitor cells from surgically created early healing alveolar defects in humans: A preliminary study. Journal of Periodontology, v. 89, n. 11, p. 1326-1333, NOV 2018. (12/14044-7)