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Variability in venom composition of Bothrops snakes and functional relevance of the presence of distinct metaloproteinases in venom composition

Grant number: 12/16277-9
Support type:Regular Research Grants
Duration: November 01, 2012 - October 31, 2014
Field of knowledge:Biological Sciences - Pharmacology - Biochemical and Molecular Pharmacology
Principal Investigator:Ana Maria Moura da Silva
Grantee:Ana Maria Moura da Silva
Home Institution: Instituto Butantan. Secretaria da Saúde (São Paulo - Estado). São Paulo , SP, Brazil

Abstract

Several aspects related to snake phylogeny, age, sex, diet and other environmental conditions interfere with protein composition in snake venoms affecting the understanding of human envenomings and the efficacy of antivenoms. Thus, the accurate knowledge of venom composition may lead to scientifically based choices of antigens for antivenoms production, molecular markers for snake taxonomy and a better prediction of clinical symptoms and treatment of patients. In this project, we aim to approach venom variability in three different levels: 1) comparing the toxin and antigen composition of venoms from snakes of 6 different species of Bothrops recently reclassified in 3 new genera; 2) characterizing the individual variability of Bothrops atrox venom composition from different geographical areas and from sympatric populations; 3) evaluating the diversity of metalloproteinases in each venom and the eventual functional variation of these toxins in different physiological systems or animal models. The results obtained by these approaches will be analyzed in the light of biodiversity, trying to elucidate taxonomic and environmental characteristics with venom diversity, and in the public health, by correlating venom variability with physiopathology of the envenomings and by supporting the antigen choice for antivenoms production. (AU)

Scientific publications (6)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
AMAZONAS, DIANA R.; PORTES-JUNIOR, JOSE A.; NISHIYAMA-JR, MILTON Y.; NICOLAU, CAROLINA A.; CHALKIDIS, HIPOCRATES M.; MOURAO, V, ROSA H.; GRAZZIOTIN, FELIPE G.; ROKYTA, DARIN R.; LISLE GIBBS, H.; VALENTE, RICHARD H.; JUNQUEIRA-DE-AZEVEDO, INACIO L. M.; MOURA-DA-SILVA, ANA M. Molecular mechanisms underlying intraspecific variation in snake venom. JOURNAL OF PROTEOMICS, v. 181, p. 60-72, JUN 15 2018. Web of Science Citations: 18.
SOUSA, LEIJIANE F.; PORTES-JUNIOR, JOSE A.; NICOLAU, CAROLINA A.; BERNARDONI, JULIANA L.; NISHIYAMA-, JR., MILTON Y.; AMAZONAS, DIANA R.; FREITAS-DE-SOUSA, LUCIANA A.; MOURAO, ROSA H. V.; CHALKIDIS, HIPOCRATES M.; VALENTE, RICHARD H.; MOURA-DA-SILVA, ANA M. Functional proteomic analyses of Bothrops atrox venom reveals phenotypes associated with habitat variation in the Amazon. JOURNAL OF PROTEOMICS, v. 159, p. 32-46, APR 21 2017. Web of Science Citations: 16.
BALDO, C.; LOPES, D. S.; FAQUIM-MAURO, E. L.; JACYSYN, J. F.; NILAND, S.; EBLE, J. A.; CLISSA, P. B.; MOURA-DA-SILVA, A. M. Jararhagin disruption of endothelial cell anchorage is enhanced in collagen enriched matrices. Toxicon, v. 108, p. 240-248, DEC 15 2015. Web of Science Citations: 9.
FREITAS-DE-SOUSA, L. A.; AMAZONAS, D. R.; SOUSA, L. F.; SANT'ANNA, S. S.; NISHIYAMA, JR., M. Y.; SERRANO, S. M. T.; JUNQUEIRA-DE-AZEVEDO, I. L. M.; CHALKIDIS, H. M.; MOURA-DA-SILVA, A. M.; MOURAO, R. H. V. Comparison of venoms from wild and long-term captive Bothrops atrox snakes and characterization of Batroxrhagin, the predominant class PIII metalloproteinase from the venom of this species. Biochimie, v. 118, p. 60-70, NOV 2015. Web of Science Citations: 25.
LIMA-DOS-SANTOS, I.; DELLA-CASA, M. S.; PORTES-JUNIOR, J. A.; CALABRIA, P. A. L.; MAGALHAES, G. S.; MOURA-DA-SILVA, A. M. Characterization of Neuwiedin, a new disintegrin from Bothrops neuwiedi venom gland with distinct cysteine pattern. Toxicon, v. 104, p. 57-64, SEP 15 2015. Web of Science Citations: 4.
BERNARDONI, JULIANA L.; SOUSA, LEIJIANE F.; WERMELINGER, LUCIANA S.; LOPES, ALINE S.; PREZOTO, BENEDITO C.; SERRANO, SOLANGE M. T.; ZINGALI, RUSSOLINA B.; MOURA-DA-SILVA, ANA M. Functional Variability of Snake Venom Metalloproteinases: Adaptive Advantages in Targeting Different Prey and Implications for Human Envenomation. PLoS One, v. 9, n. 10 OCT 14 2014. Web of Science Citations: 21.

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