Secretory structures in plant species from cerrado: structural, chemical and ecological approaches

• Grant number: 08/55434-7
• Support Opportunities: BIOTA-FAPESP Program - Thematic Grants
• Start date: November 01, 2009
• End date: October 31, 2014
• Field of knowledge: Biological Sciences - Botany - Plant Morphology
• Principal Investigator: Silvia Rodrigues Machado
• Grantee: Silvia Rodrigues Machado
• Host Institution: Instituto de Biociências (IBB). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil
• Pesquisadores principais: Luiz Claudio Di Stasi
• Associated research grant(s): 13/10416-0 - Microscopy Conference (MC) 2013, AR.EXT
• Associated scholarship(s): 13/07428-6 - Morphological, histochemical and ultrastructural diversity of glandular trichomes in Lantana camara L. (Verbenaceae) and relation between glandular morphotype and secretion, BP.IC; 11/02293-0 - Tapirira guianensis Aubl. (Anacardiaceae): an investigation about the secretions produced in flower and fruit, BP.MS; 10/15153-9 - Influence of exogenous factors on the oleoresin secretory system of Copaifera langsdorffii Desf. (Leguminosae-Ceaslpinioideae)from cerrado, BP.IC; 09/17642-0 - Distribution and Characterization of Scent Glands in Species of Leguminosae, BP.IC

Abstract

This proposal aims to gather knowledge on different aspects of the secretion biology and chemistry features in plant species from Cerrado, belonging to Apocynaceae, Bignoniaceae, Bromeliaceae, Fabaceae, Malpighiaceae and Rubiaceae families. We have to stress that the secretory structures play essential role in the production and isolation of secondary metabolites that play several functions on biological interactions. Multidisciplinary researches about secretory structures give subsidies to community structures analyses and can give essential information to defense and attraction mechanisms studies on plant-animal interactions. Thus, structural analyses will be performed following conventional techniques to studies on Iight microscope and scanning and transmission electron microscope. To detect in situ the main substances categories present in secretory tisues/cells, histochemical, citochemical and immunohistochemical tests will be employed, besides ultra structural citochemistry and biochemical analyses. The chemical profile of the secretion will be analyzed using thin-Iayer chromatography and gas chromatography-mass spectrometry (GC/MS). The function of the secretion produced by external glands on the plant-animal interactions will be evaluated through field observations and experiments. At the end of the project we intend to summarize the results with an integrated approach about the biology and chemistry of secretory structures in Cerrado species. Besides it, we look forward to that this multidisciplinary research may contribute to evaluate the economic potential of bioactive and its sustainable utilization. In addition, the results could aid in the strategies definition of conservation and management of biological diversity in fragmented areas, as occur in Cerrado regions from São Paulo State. (AU)

Scientific publications

(33)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

GUIMARAES, E.; NOGUEIRA, A.; MACHADO, S. R.. Floral nectar production and nectary structure of a bee-pollinated shrub from Neotropical savanna. Plant Biology, v. 18, n. 1, SI, p. 26-36, JAN 2016. (08/55434-7, 12/02110-5)

MACHADO, SILVIA R.; PALEARI, LUCIA M.; PAIVA, ELDER A. S.; RODRIGUES, TATIANE M.. COLLETERS ON THE INFLORESCENCE AXIS OF CROTON GLANDULOSUS (EUPHORBIACEAE): STRUCTURAL AND FUNCTIONAL CHARACTERIZATION. INTERNATIONAL JOURNAL OF PLANT SCIENCES, v. 176, n. 1, p. 86-93, JAN 2015. (08/55434-7)

CANAVEZE, YVE; MACHADO, SILVIA RODRIGUES. Leaf colleters in Tabernaemontana catharinensis (Apocynaceae, Rauvolfioideae): structure, ontogenesis, and cellular secretion. BOTANY, v. 93, n. 5, p. 287-296, MAY 2015. (08/55434-7)

DOS SANTOS TOZIN, LUIZ RICARDO; CARVALHO, SHELLY FAVORITO; MACHADO, SILVIA RODRIGUES; RODRIGUES, TATIANE MARIA. Glandular trichome diversity on leaves of Lippia origanoides and Lippia stachyoides (Verbenaceae): morphology, histochemistry, and ultrastructure. BOTANY, v. 93, n. 5, p. 297-306, MAY 2015. (08/55434-7)

ADACHI, SERGIO AKIRA; MACHADO, SILVIA RODRIGUES; GUIMARAES, ELZA. Structural and ultrastructural characterization of the floral lip in Gongora bufonia (Orchidaceae): understanding the slip-and-fall pollination mechanism. BOTANY, v. 93, n. 11, p. 759-768, NOV 2015. (08/55434-7)

RODRIGUES, TATIANE MARIA; DOS SANTOS, DANIELA CARVALHO; MACHADO, SILVIA RODRIGUES. The role of the parenchyma sheath and PCD during the development of oil cavities in Pterodon pubescens (Leguminosae-Papilionoideae). COMPTES RENDUS BIOLOGIES, v. 334, n. 7, p. 535-543, JUL 2011. (05/60086-0, 08/55434-7)

MILANI, JULIANA FORESTI; ROCHA, JOECILDO FRANCISCO; TEIXEIRA, SIMONE DE PADUA. Oleoresin glands in copaiba (Copaifera trapezifolia Hayne: Leguminosae), a Brazilian rainforest tree. TREES-STRUCTURE AND FUNCTION, v. 26, n. 3, p. 769-775, JUN 2012. (08/55434-7)

MACHADO, SILVIA RODRIGUES; BARREIRO, DANIELA PACHECO; ROCHA, JOECILDO FRANCISCO; RODRIGUES, TATIANE MARIA. Dendroid colleters on vegetative and reproductive apices in Alibertia sessilis (Rubiaceae) differ in ultrastructure and secretion. FLORA, v. 207, n. 12, p. 868-877, 2012. (03/11747-8, 08/55434-7)

SA-HAIAD, B.; SILVA, C. P.; PAULA, R. C. V.; ROCHA, J. F.; MACHADO, S. R.. Androecia in two Clusia species: development, structure and resin secretion. Plant Biology, v. 17, n. 4, p. 816-824, JUL 2015. (08/55434-7)

DE BARROS, THAIS CURY; TEIXEIRA, SIMONE PADUA. Morphology and ontogeny of tannin-producing structures in two tropical legume trees. BOTANY, v. 92, n. 7, p. 513-521, JUL 2014. (08/07392-3, 08/55434-7)

SOUZA, CAMILA VAZ; NEPI, MASSIMO; MACHADO, SILVIA RODRIGUES; GUIMARAES, ELZA. Floral biology, nectar secretion pattern and fruit set of a threatened Bignoniaceae tree from Brazilian tropical forest. FLORA, v. 227, p. 46-55, FEB 2017. (08/55434-7)

MARINHO, CRISTINA RIBEIRO; POLETI MARTUCCI, MARIA ELVIRA; GOBBO-NETO, LEONARDO; TEIXEIRA, SIMONE PADUA. Chemical composition and secretion biology of the floral bouquet in legume trees (Fabaceae). Botanical Journal of the Linnean Society, v. 187, n. 1, p. 5-25, MAY 2018. (09/01057-0, 12/16646-4, 08/55434-7)

MACHADO, SILVIA RODRIGUES; CANAVEZE, YVE; RODRIGUES, TATIANE MARIA. Structure and functioning of oil cavities in the shoot apex of Metrodorea nigra A. St.-Hil. (Rutaceae). PROTOPLASMA, v. 254, n. 4, p. 1661-1674, JUL 2017. (08/55434-7)

CLIVIA CAROLINA FIORILO POSSOBOM; SILVIA RODRIGUES MACHADO. Elaiophores: their taxonomic distribution, morphology and functions. Acta Botanica Brasilica, v. 31, n. 3, p. 503-524, 2017-05-15. (03/04207-7, 08/55434-7)

QUINALHA, MARILIA MONTEIRO; NOGUEIRA, ANSELMO; FERREIRA, GISELA; GUIMARAES, ELZA. Effect of mutualistic and antagonistic bees on floral resources and pollination of a savanna shrub. FLORA, v. 232, p. 30-38, JUL 2017. (08/55434-7)

SILVA MELO BUARQUE, PLACIDO FABRICIO; MACHADO, SILVIA RODRIGUES; RODRIGUES, TATIANE MARIA. Anatomical and ultrastructural studies reveal temporal and spatial variation in the oil production in leaves of the diesel tree (Copaifera langsdorffii, Leguminosae). PROTOPLASMA, v. 257, n. 5, p. 1447-1456, SEP 2020. (08/55434-7)

CANAVEZE, YVE; MASTROBERTI, ALEXANDRA ANTUNES; DE ARAUJO MARIATH, JORGE ERNESTO; MACHADO, SILVIA RODRIGUES. Cytological differentiation and cell wall involvement in the growth mechanisms of articulated laticifers in Tabernaemontana catharinensis A.DC. (Apocynaceae). PROTOPLASMA, v. 256, n. 1, p. 131-146, JAN 2019. (12/16441-3, 08/55434-7)

GUIMARAES, ELZA; TUNES, PRISCILA; DE ALMEIDA JUNIOR, LUIZ D.; DI STASI, LUIZ C.; DOETTERL, STEFAN; MACHADO, SILVIA R.. Nectar Replaced by Volatile Secretion: A Potential New Role for Nectarless Flowers in a Bee-Pollinated Plant Species. FRONTIERS IN PLANT SCIENCE, v. 9, SEP 5 2018. (08/55434-7)

TRESMONDI, FERNANDA; CANAVEZE, YVE; GUIMARAES, ELZA; MACHADO, SILVIA RODRIGUES. Colleters in Rubiaceae from forest and savanna: the link between secretion and environment. NATURWISSENSCHAFTEN, v. 104, n. 3-4, APR 2017. (11/02488-5, 08/55434-7)

MACHADO, SILVIA RODRIGUES; DE SOUZA, CAMILA VAZ; GUIMARAES, ELZA. A reduced, yet functional, nectary disk integrates a complex system of floral nectar secretion in the genus Zeyheria (Bignoniaceae). Acta Botanica Brasilica, v. 31, n. 3, p. 344-357, JUL-SEP 2017. (08/55434-7)

DOS SANTOS TOZIN, LUIZ RICARDO; DE MELO SILVA, STEFANY CRISTINA; RODRIGUES, TATIANE MARIA. Non-glandular trichomes in Lamiaceae and Verbenaceae species: morphological and histochemical features indicate more than physical protection. NEW ZEALAND JOURNAL OF BOTANY, v. 54, n. 4, p. 446-457, 2016. (13/07428-6, 08/55434-7)

TRESMONDI, FERNANDA; NOGUEIRA, ANSELMO; GUIMARAES, ELZA; MACHADO, SILVIA RODRIGUES. Morphology, secretion composition, and ecological aspects of stipular colleters in Rubiaceae species from tropical forest and savanna. NATURWISSENSCHAFTEN, v. 102, n. 11-12, DEC 2015. (11/02488-5, 08/55434-7)

FERNANDA HELENA PALERMO; SIMONE DE PÁDUA TEIXEIRA; VIDAL DE FREITAS MANSANO; VIVIANE GONÇALVES LEITE; TATIANE MARIA RODRIGUES. Secretory spaces in species of the clade Dipterygeae (Leguminosae, Papilionoideae). Acta Botanica Brasilica, v. 31, n. 3, p. 374-381, 2017-01-30. (12/15644-8, 08/55434-7)

TOZIN, LUIZ R. S.; MARQUES, MARCIA O. M.; RODRIGUES, TATIANE M.. Glandular trichome density and essential oil composition in leaves and inflorescences of Lippia origanoides Kunth (Verbenaceae) in the Brazilian Cerrado. Anais da Academia Brasileira de Ciências, v. 87, n. 2, p. 943-953, JUN 2015. (08/55434-7)

CANAVEZE, YVE; MACHADO, SILVIA RODRIGUES. The Occurrence of Intrusive Growth Associated with Articulated Laticifers in Tabernaemontana catharinensis A.DC., a New Record for Apocynaceae. INTERNATIONAL JOURNAL OF PLANT SCIENCES, v. 177, n. 5, p. 458-467, JUN 2016. (12/16441-3, 08/55434-7)

MACHADO, SILVIA RODRIGUES; TEIXEIRA, SIMONE DE PADUA; RODRIGUES, TATIANE MARIA. Bacterial leaf glands in Styrax camporum (Styracaceae): first report for the family. BOTANY, v. 92, n. 5, p. 403-411, MAY 2014. (08/55434-7)

POSSOBOM, CLIVIA C. F.; MACHADO, SILVIA R.. Elaiophores in three Neotropical Malpighiaceae species: a comparative study. PLANT SYSTEMATICS AND EVOLUTION, v. 304, n. 1, p. 15-32, JAN 2018. (08/55434-7)

MARINHO, CRISTINA RIBEIRO; OLIVEIRA, REJANE BARBOSA; TEIXEIRA, SIMONE PADUA. The uncommon cavitated secretory trichomes in Bauhinia s.s. (Fabaceae): the same roles in different organs. Botanical Journal of the Linnean Society, v. 180, n. 1, p. 104-122, JAN 2016. (09/01057-0, 08/55434-7)

FIORILO POSSOBOM, CLIVIA CAROLINA; GUIMARAES, ELZA; MACHADO, SILVIA RODRIGUES. Structure and secretion mechanisms of floral glands in Diplopterys pubipetala (Malpighiaceae), a neotropical species. FLORA, v. 211, p. 26-39, 2015. (08/55434-7)

RODRIGUES, T. M.; BUARQUE, P. F. S. M.; CONEGLIAN, A. G.; REIS, D. C.. Light and temperature induce variations in the density and ultrastructure of the secretory spaces in the diesel-tree (Copaifera langsdorffii Desf.-Leguminosae). TREES-STRUCTURE AND FUNCTION, v. 28, n. 2, p. 613-623, APR 2014. (10/15153-9, 08/55434-7)

MARINHO, C. R.; SOUZA, C. D.; BARROS, T. C.; TEIXEIRA, S. P.. Scent glands in legume flowers. Plant Biology, v. 16, n. 1, p. 215-226, JAN 2014. (09/01057-0, 09/17642-0, 08/55434-7)

DE SOUZA, CAMILA DEVICARO; MARINHO, CRISTINA RIBEIRO; TEIXEIRA, SIMONE PADUA. Ontogeny resolves gland classification in two caesalpinoid legumes. TREES-STRUCTURE AND FUNCTION, v. 27, n. 3, p. 801-813, JUN 2013. (09/01057-0, 09/17642-0, 08/55434-7)

GUIMARAES, E.; NOGUEIRA, A.; MACHADO, S. R.. Floral nectar production and nectary structure of a bee-pollinated shrub from Neotropical savanna. Plant Biology, v. 18, n. 1, p. 11-pg., 2016-01-01. (12/02110-5, 08/55434-7)