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Probiotics on Nile Tilapia, Oreochromis niloticus feeding

Grant number: 13/09731-8
Support type:Regular Research Grants
Duration: August 01, 2013 - July 31, 2016
Field of knowledge:Agronomical Sciences - Fishery Resources and Fishery Engineering
Principal Investigator:Leonardo Tachibana
Grantee:Leonardo Tachibana
Home Institution: Instituto de Pesca. Agência Paulista de Tecnologia dos Agronegócios (APTA). Secretaria de Agricultura e Abastecimento (São Paulo - Estado). São Paulo , SP, Brazil
Assoc. researchers: Carlos Massatoshi Ishikawa ; Danielle de Carla Dias ; Fábio Rosa Sussel ; Giovani Sampaio Gonçalves ; Guilherme Silveira Telli ; Maria José Tavares Ranzani de Paiva

Abstract

The project will be conducted with Nile tilapia, Oreochromis niloticus, at APTA / Pólo - East Central / UPD Pirassununga / SP. The project will be divided into three phases which aim to evaluate the parameters of zoothecnical performance, microbiology, hematology, immunology, food digestibility, digestive enzymes with use of two commercial probiotics tested separately - Bioplus 2BC (Bacillus subtilis - (1.6 x1010 CFU g -1) and Bacillus licheformis - (1,6 x1010 CFU g-1) and DBA (Lactobacillus acidophilus - 3.5 x109 CFU g-1, Enterococcus faecium - 3.5 x109 CFU g-1 and Bifidobacteria - 3.5 x109 CFU g-1 ) included in the diet of Nile tilapia in the same concentrations for both probiotics of 0.00, 0.02, 0.04 and 0.08%. During the first phase will be determined the apparent digestibility of dry matter, protein gross, fat, phosphorus and gross energy of diets with probiotics in different dosages, in a completely randomized experimental design with four treatments and three replications. During the second phase will be performed to evaluate the growth performance, hematological and immunological parameters, concentration of digestive enzymes, body composition of fish, intestinal microbiota (DGGE) of Nile tilapia fed diets containing concentrations of probiotics for period of 84 days. the completely randomized design with four treatments and four replications. The third phase the fish (± 25 g) will be experimentally infected "in vivo" with Aeromonas hydrophila after 21 days of feeding with diets containing different levels of probiotic in a is completely randomized experimental design with four treatments and three replications. index will be observed and protection for clinical signs of infection with A. hydrophila 15 days post-infection. (AU)