Isolation, identification and development of molecular marks for evaluation of Aspergillus niger producers of fumonisin B2 and ochratoxin A

• Grant number: 13/05414-8
• Support Opportunities: Regular Research Grants
• Start date: October 01, 2013
• End date: March 31, 2016
• Field of knowledge: Agronomical Sciences - Food Science and Technology - Food Science
• Principal Investigator: Beatriz Thie Iamanaka
• Grantee: Beatriz Thie Iamanaka
• Host Institution: Instituto de Tecnologia de Alimentos (ITAL). Agência Paulista de Tecnologia dos Agronegócios (APTA). Secretaria de Agricultura e Abastecimento (São Paulo - Estado). Campinas , SP, Brazil
• Associated researchers: Maria Helena Pelegrinelli Fungaro ; Marta Hiromi Taniwaki

Abstract

Aspergillus niger is a fungus, belonging to the Nigri setion, which is of high biotechnological importance. However, it has been described that this species has the potential to produce two groups of mycotoxins harmful to human and animal health: fumonisin B2 and ochratoxin A. In Brazil, the knowledge of the incidence and genetic characterization of isolates of A. niger producers and non-producers of mycotoxins, particularly fumonisin, is very restricted. There is an overriding need for studies that reveal the incidence of toxigenic A. niger in Brazilian agribusiness products, in order to understand the damage they cause and outline preventive control measures. The objective of this project is to isolate and identify Aspergillus section Nigri in grape and onion produced in Brazil, and to evaluate the presence of ochratoxin A and fumonisin B2 in these foods. Isolates belonging to the species A. niger will be investigated for their ability to produce ochratoxin and fumonisin. Once the producer and non- producer strains of these toxins mentioned are identified, they will be used to study genetic variation in orthologous gene clusters in those previously described for Fusarium verticillioides (fumonisin biosynthesis) and A. ochraceus (ochratoxin biosynthesis). Genes that are present in producer strains and are absent in non- producer strains will be used for the development of detection systems specific for strains of A. niger producers of ochratoxin and /or fumonisin. The development of a molecular detection method directly (without cultivation) for monitoring A. niger mycotoxin producers will also be studied. (AU)

Scientific publications

(10)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

MASSI, FERNANDA PELISSON; IAMANAKA, BEATRIZ THIE; BARBOSA, RAFAELLA LIVIERO; SARTORI, DANIELE; FERRRANTI, LARISSA; TANIWAKI, MARTA HIROMI; FUNGARO, MARIA HELENA PELEGRINELLI. olecular analysis of Aspergillus section Nigri isolated from onion samples reveals the prevalence of A. welwitschia. Brazilian Journal of Microbiology, v. 52, n. 1, SI, OCT 2020. (13/05414-8)

MASSI, FERNANDA PELISSON; SARTORI, DANIELE; FERRANTI, LARISSA DE SOUZA; IAMANAKA, BEATRIZ THIE; TANIWAKI, MARTA HIROMI; CARNEIRO VIEIRA, MARIA LUCIA; PELEGRINELLI FUNGARO, MARIA HELENA. Prospecting for the incidence of genes involved in ochratoxin and fumonisin biosynthesis in Brazilian strains of Aspergillus niger and Aspergillus welwitschiae. International Journal of Food Microbiology, v. 221, p. 19-28, MAR 16 2016. (13/05414-8)

SILVA, JOSUE JOSE; BERTOLDO, RACHEL; PELEGRINELLI FUNGARO, MARIA HELENA; MASSI, FERNANDA PELISSON; TANIWAKI, MARTA HIROMI; SANT'ANA, ANDERSON S.; IAMANAKA, BEATRIZ THIE. Black aspergilli in Brazilian onions: From field to market. International Journal of Food Microbiology, v. 337, JAN 16 2021. (18/25597-3, 13/05414-8)

MASSI, FERNANDA PELISSON; IAMANAKA, BEATRIZ THIE; BARBOSA, RAFAELLA LIVIERO; SARTORI, DANIELE; FERRRANTI, LARISSA; TANIWAKI, MARTA HIROMI; FUNGARO, MARIA HELENA PELEGRINELLI. Molecular analysis of Aspergillus section Nigri isolated from onion samples reveals the prevalence of A. welwitschiae. Brazilian Journal of Microbiology, v. 52, n. 1, p. 6-pg., 2020-10-22. (13/05414-8)

ONO, L. T.; SILVA, J. J.; DONA, S.; MARTINS, L. M.; IAMANAKA, B. T.; FUNGARO, M. H. P.; PITT, J. I.; TANIWAKI, M. H.. Aspergillus section Flavi and aflatoxins in Brazilian cassava (Manihot esculenta Crantz) and products. MYCOTOXIN RESEARCH, v. 37, n. 3, p. 221-228, AUG 2021. (18/25552-0, 18/25597-3, 13/05414-8)

FERRANTI, LARISSA DE SOUZA; FUNGARO, MARIA HELENA P.; MASSI, FERNANDA PELISSON; DA SILVA, JOSUE JOSE; SILVA PENHA, RAFAEL ELIAS; FRISVAD, JENS CHRISTIAN; TANIWAKI, MARTA HIROMI; IAMANAKA, BEATRIZ THIE. Diversity of Aspergillus section Nigri on the surface of Vitis labrusca and its hybrid grapes. International Journal of Food Microbiology, v. 268, p. 53-60, MAR 2 2018. (13/05414-8)

DA SILVA, JOSUE J.; IAMANAKA, BEATRIZ T.; FERRANTI, LARISSA S.; MASSI, FERNANDA P.; TANIWAKI, MARTA H.; PUEL, OLIVIER; LORBER, SOPHIE; FRISVAD, JENS C.; FUNGARO, MARIA HELENA P.. Diversity within Aspergillus niger Clade and Description of a New Species: Aspergillus vinaceus sp. nov.. JOURNAL OF FUNGI, v. 6, n. 4, DEC 2020. (13/05414-8)

ONO, L. T.; SILVA, J. J.; SOTO, T. S.; DONA, S.; IAMANAKA, B. T.; FUNGARO, M. H. P.; TANIWAKI, M. H.. Fungal communities in Brazilian cassava tubers and food products. International Journal of Food Microbiology, v. 384, p. 10-pg., 2023-01-02. (18/25552-0, 13/05414-8, 18/25597-3)

MASSI, FERNANDA PELISSON; SARTORI, DANIELE; FERRANTI, LARISSA DE SOUZA; IAMANAKA, BEATRIZ THIE; TANIWAKI, MARTA HIROMI; CARNEIRO VIEIRA, MARIA LUCIA; PELEGRINELLI FUNGARO, MARIA HELENA. Data on the presence or absence of genes encoding essential proteins for ochratoxin and fumonisin biosynthesis in Aspergillus niger and Aspergillus welwitschiae. DATA IN BRIEF, v. 7, p. 704-708, JUN 2016. (13/05414-8)

FUNGARO, MARIA HELENA P.; FERRANTI, LARISSA S.; MASSI, FERNANDA P.; DA SILVA, JOSUE J.; SARTORI, DANIELE; TANIWAKI, MARTA H.; FRISVAD, JENS C.; IAMANAKA, BEATRIZ T.. Aspergillus labruscus sp nov., a new species of Aspergillus section Nigri discovered in Brazil. SCIENTIFIC REPORTS, v. 7, JUL 24 2017. (13/05414-8)