Grape diseases: epidemiology, damage assessment and control

• Grant number: 13/24003-9
• Support Opportunities: Research Projects - Thematic Grants
• Duration: June 01, 2014 - October 31, 2019
• Field of knowledge: Agronomical Sciences - Agronomy - Plant Health
• Principal Investigator: Lilian Amorim
• Grantee: Lilian Amorim
• Host Institution: Escola Superior de Agricultura Luiz de Queiroz (ESALQ). Universidade de São Paulo (USP). Piracicaba , SP, Brazil
• Pesquisadores principais: Marcel Bellato Spósito
• Associated researchers: Armando Bergamin Filho ; Beatriz Appezzato da Glória ; Claudia Barros Monteiro Vitorello ; José Belasque Junior ; Marco Loehrer ; Paulo Cesar Sentelhas ; Rafael Vasconcelos Ribeiro ; Ulrich Schaffrath
• Associated grant(s): 14/20763-1 - Multi-User Equipment approved in grant 2013/24003-9: infrared gas analyser - IRGA for photosynthesis analyses, AP.EMU
• Associated scholarship(s): 19/15191-2 - Can the stress caused by temperature affect the grapevine rust pathosystem? Epidemiologic, physiological and histopathological analyses in Vitis labrusca 'Niagara Rosada' and V. vinifera 'Cabernet', BP.MS; 17/25407-7 - Monitoring of Phakopsora euvitis urediniospores and temporal analysis of grapevine rust on plastic covered plants and uncovered plants, BP.IC; 17/21412-6 - Population structure and sensitivity of Plasmopara viticola and Phakopsora euvitis to fungicides in Brazil, BP.PD; + associated scholarships 17/10197-7 - Ultrastrutural studies of the interaction between Phakpsora euvitis and leaves of Vitis labrusca L. Niagara Rosada, BP.IC; 17/07782-5 - Sensitivity of isolates of Plasmopara viticola to strobilurins in the State of São Paulo and their relationship to downy mildew management, BP.IC; 17/02432-6 - Simulation modeling of damage caused by downy mildew and grapevine rust in Vitis spp. under different training systems, BP.PD; 15/26108-8 - Aggressiveness of Phakopsora: a comparative analysis of grapevine and soybean rusts with emphasis to histopathology and epidemiology, BP.DR; 14/19711-7 - Variability analysis of gene cytB on the genomic region associated with resistance to QoI fungicides on pathogens of Niagara grapes (Vitis labrusca), BP.MS; 15/03735-7 - Sensitivity of Sphaceloma ampelinum isolates to fungicides and relationship among environmental variables and progress of anthracnose in Niagara Rosada, BP.IC; 14/21094-6 - Phakopsora euvitis isolates sensitivity to fungicides and environmental-related variables and grapevine rust progress in Niagara Rosada grapes, BP.IC; 14/17328-1 - Sensitivity of Plasmopara viticola isolates to fungicides and relationship among environmental variables and progress of grape downy mildew in Niagara Rosada, BP.IC - associated scholarships

Abstract

This research project was motivated by the exaggerated number of fungicides spraying by the growers of São Paulo State for the control of grape diseases. Recent estimates indicate that the control of fungal diseases has been performed with 103 and 59 fungicide spraying each season in vineyards of european (Vitis vinifera) and american grapes (Vitis labrusca), respectively, in the State of São Paulo (Costa et al ., 2012). It is hard to understand the reasoning for this huge number of fungicide spraying as the damage caused by grape diseases that occur in São Paulo were not quantitatively estimated. In addition there are no detailed information about the influence of environmental variables on the diseases development (except for downy mildew) in São Paulo. This intensive and protective disease management increases the grapes production costs and can lead to the selection of resistant strains of pathogens to fungicides, to the accumulation of fungicides in the soil, and to the presence of fungicide residues in grapes, with damages not yet estimated to the environment and the consumer. Different strategies for disease management must be performed in São Paulo to reduce the number of fungicide applications without reducing the efficiency of control. The main objective of this research project is to understand the epidemic process of mildew, anthracnose and rust in São Paulo vineyards, in order to propose management strategies to reduce the use of fungicides. In addition to the study of the most frequent diseases in São Paulo, this project will also assess the development of bacterial canker of grapevine, a disease restricted to the Brazilian Northeast, and estimate the damage caused by this disease, in order to anticipate its impact before its introduction in the State of São Paulo. It is proposed in this project: to evaluate the monocycle of grape rust, grape anthracnose and bacterial canker of grapevine under controlled conditions; to evaluate the effects of these diseases and downy mildew on photosynthesis and on the distribution of vine assimilates for five years, in order to estimate the damage caused by these disease on the grapes; to determine the sensitivity of Plasmopara viticola, Phakopsora euvitis e Elsinoë ampelina (Sphaceloma ampelinum) to fungicides and molecular characterization of the isolates; to characterize the effect of plant architecture on the environmental variables and on the spatial-temporal distribution of diseases in vineyards; to simulate the progress of each disease; to proposed forecast and warning systems for the control of these diseases. The research team has experts on plant diseases epidemiology, fruit production, histopathology, plant physiology, agrometeorology and disease forecast. (AU)

Scientific publications

(28)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

SANTOS, RICARDO F.; PRIMIANO, V, ISABELA; AMORIM, LILIAN. Identification and pathogenicity ofNeophysopellaspecies associated with Asian grapevine leaf rust in Brazil. PLANT PATHOLOGY, v. 70, n. 1, p. 74-86, JAN 2021. (15/26108-8, 13/24003-9, 17/21412-6)

RASERA, J. B.; AMORIM, L.; MARQUES, J. P. R.; SOARES, M. K. M.; APPEZZATO-DA-GLORIA, B.. Histopathological evidences of early grapevine leaf senescence caused by Phakopsora euvitis colonisation. PHYSIOLOGICAL AND MOLECULAR PLANT PATHOLOGY, v. 108, DEC 2019. (13/24003-9, 17/10197-7)

PRIMIANO, V, ISABELA; AMORIM, LILIAN. Comparative study on the monocycle of Phakopsora meliosmae-myrianthae and Phakopsora pachyrhizi. European Journal of Plant Pathology, v. 157, n. 1, MAY 2020. (15/26108-8, 13/24003-9)

NOGUEIRA JUNIOR, ANTONIO F.; RIBEIRO, RAFAEL V.; MARCOS, FERNANDA C. C.; AMORIM, LILIAN. Virtual lesions and photosynthetic damage caused by Plasmopara viticola in Vitis labrusca. European Journal of Plant Pathology, v. 155, n. 2, p. 545-555, OCT 2019. (17/02432-6, 13/24003-9)

SANCHEZ-RODRIGUEZ, LUZ ANGELA; SPOSITO, MARCEL BELLATO. Influence of the trellis/training system on the physiology and production of Vitis labrusca cv. Niagara Rosada in Brazil. Scientia Horticulturae, v. 261, FEB 5 2020. (13/24003-9, 14/08190-6)

SANTOS, RICARDO F.; FRAAIJE, BART A.; GARRIDO, LUCAS DA R.; MONTEIRO-VITORELLO, CLAUDIA B.; AMORIM, LILIAN. Multiple resistance ofPlasmopara viticolato QoI and CAA fungicides in Brazil. PLANT PATHOLOGY, v. 69, n. 9, AUG 2020. (18/25596-7, 17/21412-6, 13/24003-9)

SANTOS, RICARDO F.; AMORIM, LILIAN; WOOD, ANA K. M.; BIBIANO, LILLIAN B. J.; FRAAIJE, BART A.. Lack of an Intron in Cytochrome b and Overexpression of Sterol 14 alpha-Demethylase Indicate a Potential Risk for QoI and DMI Resistance Development in Neophysopella spp. on Grapes. PHYTOPATHOLOGY, v. 111, n. 10, p. 1726-1734, OCT 2021. (17/21412-6, 18/25596-7, 13/24003-9)

RASERA, J. B.; AMORIM, L.; APPEZZATO-DA-GLORIA, B.. iverse effects of temperature on in vivo and in vitro germination of urediniospores of Neophysopella tropicali. European Journal of Plant Pathology, v. 162, n. 2, OCT 2021. (19/13191-5, 13/24003-9, 19/15191-2)

NOGUEIRA JUNIOR, ANTONIO F.; TRAENKNER, MERLE; RIBEIRO, V, RAFAEL; VON TIEDEMANN, ANDREAS; AMORIM, LILIAN. Photosynthetic Cost Associated With Induced Defense to Plasmopara viticola in Grapevine. FRONTIERS IN PLANT SCIENCE, v. 11, MAR 19 2020. (18/00952-5, 17/02432-6, 13/24003-9)

DOS SANTOS, RICARDO FELICIANO; SPOSITO, MARCEL BELLATO; AYRES, MATTHEW RONALD; SOSNOWSKI, MARK ROMAN. Phylogeny, morphology and pathogenicity of Elsinoe ampelina, the causal agent of grapevine anthracnose in Brazil and Australia. JOURNAL OF PHYTOPATHOLOGY, v. 166, n. 3, p. 187-198, MAR 2018. (13/24003-9, 14/24472-1, 16/01508-6)

DOS SANTOS, RICARDO FELICIANO; AMORIM, LILIAN; ZINI, PRISCILA BARBIERI; SPOSITO, MARCEL BELLATO. Temporal and spatial dynamics of grapevine anthracnose and its relationship to pathogen survival. JOURNAL OF PHYTOPATHOLOGY, v. 168, n. 7-8, MAY 2020. (14/24472-1, 13/24003-9)

ALVES, RENAN FERNANDES; MASSOLA JUNIOR, NELSON SIDNEI; SPOSITO, MARCEL BELLATO. Preservation of the urediniospores of Phakopsora euvitis, the causal agent of grape leaf rust. European Journal of Plant Pathology, v. 150, n. 4, p. 1103-1106, APR 2018. (13/24003-9)

PRIMIANO, V, I.; LOEHRER, M.; SCHAFFRATH, U.; AMORIM, L.. Formation of satellite uredinia as an important trait related to grapevine colonization by Phakopsora meliosmae-myrianthae. PLANT PATHOLOGY, v. 68, n. 9, p. 1732-1740, DEC 2019. (15/26108-8, 13/24003-9, 16/25111-8)

SANTOS, R. F.; SPOSITO, M. B.. Improving assessments of anthracnose severity on grapevine leaves through the development of a standard area diagram set. AUSTRALASIAN PLANT PATHOLOGY, v. 47, n. 4, p. 357-364, JUL 2018. (13/24003-9, 14/24472-1)

NOGUEIRA JUNIOR, ANTONIO F.; AMORIM, LILIAN; SAVARY, SERGE; WILLOCQUET, LAETITIA. Modelling the dynamics of grapevine growth over years. ECOLOGICAL MODELLING, v. 369, p. 77-87, FEB 10 2018. (13/24003-9)

CAPPELLO, FERNANDO PEREZ; SPOSITO, MARCEL BELLATO; OSAKI, MAURO. PRODUCTION COSTS AND PROFITABILITY OF `NIAGARA ROSADA' TABLE GRAPE GROWN IN DIFFERENT REGIONS OF SAO PAULO STATE. Revista Brasileira de Fruticultura, v. 39, n. 4, 2017. (13/24003-9)

SANTOS, R. F.; CIAMPI-GUILLARDI, M.; AMORIM, L.; MASSOLA JUNIOR, N. S.; SPOSITO, M. B.. Aetiology of anthracnose on grapevine shoots in Brazil. PLANT PATHOLOGY, v. 67, n. 3, p. 692-706, APR 2018. (13/24003-9, 14/24472-1)

PRIMIANO, I. V.; LOEHRER, M.; AMORIM, L.; SCHAFFRATH, U.. Asian grapevine leaf rust caused by Phakopsora euvitis: an important disease in Brazil. PLANT PATHOLOGY, v. 66, n. 5, p. 691-701, JUN 2017. (13/24003-9, 15/26108-8)

NOGUEIRA JUNIOR, ANTONIO F.; RIBEIRO, RAFAEL V.; APPEZZATO-DA-GLORIA, BEATRIZ; SOARES, MARLI K. M.; RASERA, JULIA B.; AMORIM, LILIAN. Phakopsora euvitis Causes Unusual Damage to Leaves and Modifies Carbohydrate Metabolism in Grapevine. FRONTIERS IN PLANT SCIENCE, v. 8, SEP 26 2017. (13/24003-9)

LUZ ANGELA SANCHEZ-RODRIGUEZ; CARLOS TADEU DOS SANTOS DIAS; MARCEL BELLATO SPÓSITO. Fisiologia e produção da videira 'Niágara Rosada' nos sistemas de condução em espaldeira e em Y. Pesquisa Agropecuária Brasileira, v. 51, n. 12, p. 1948-1956, 2016-12-00. (14/08190-6, 13/24003-9)

DOS SANTOS, RICARDO FELICIANO; SPOSITO, MARCEL BELLATO; AYRES, MATTHEW; SOSNOWSKI, MARK. In vitro production of conidia of Elsinoe ampelina, the causal fungus of grapevine anthracnose. European Journal of Plant Pathology, v. 152, n. 3, p. 815-821, NOV 2018. (14/24472-1, 16/01508-6, 13/24003-9)

BRAGA, ZELIA VALENTE; MUNIZ, LARISSA FERNANDA; MANARIM, GISLENE ROBERTA; DE AGUIAR, CLAUDIO LIMA; APPEZZATO-DA-GLORIA, BEATRIZ. Anatomical and biochemical changes in leaves of Vitis labrusca L. cv. Niagara Rosada in response to infection by Elsinoe ampelina Shear. Rev. bras. Bot., v. 44, n. 1, p. 187-196, MAR 2021. (13/24003-9)

PRIMIANO, ISABELA V.; CIA, MARIANA C.; CAMARGO, LUIS E. A.; AMORIM, LILIAN. Early detection of Neophysopella tropicalis in grapevine leaves and on spore traps by qPCR. PLANT PATHOLOGY, v. 70, n. 2, OCT 2020. (13/24003-9)

BRAGA, ZELIA VALENTE; DOS SANTOS, RICARDO FELICIANO; AMORIM, LILIAN; APPEZZATO-DA-GLORIA, BEATRIZ. Histopathology of infection and colonisation of Elsinoe ampelina on grapevine leaves. European Journal of Plant Pathology, v. 154, n. 4, p. 1009-1019, AUG 2019. (13/24003-9)

NAVARRO, BARBARA LUDWIG; RODRIGUES MARQUES, JOAO PAULO; APPEZZATO-DA- GLORIA, BEATRIZ; SPOSITO, MARCEL BELLATO. Histopathology of Phakopsora euvitis on Vitis vinifera. European Journal of Plant Pathology, v. 154, n. 4, p. 1185-1193, AUG 2019. (13/24003-9)

NAVARRO, B. L.; NOGUEIRA JUNIOR, A. F.; RIBEIRO, R. V.; SPOSITO, M. B.. Photosynthetic damage caused by grapevine rust (Phakopsora euvitis) in Vitis vinifera and Vitis labrusca. AUSTRALASIAN PLANT PATHOLOGY, v. 48, n. 5, p. 509-518, SEP 2019. (17/02432-6, 13/24003-9)

SANTOS, RICARDO F.; CIAMPI-GUILLARDI, MAISA; FRAAIJE, BART A.; DE OLIVEIRA, AMANDA A.; AMORIM, LILIAN. The Climate-Driven Genetic Diversity Has a Higher Impact on the Population Structure ofPlasmopara viticolaThan the Production System or QoI Fungicide Sensitivity in Subtropical Brazil. FRONTIERS IN MICROBIOLOGY, v. 11, SEP 17 2020. (13/24003-9, 17/21412-6, 18/25596-7, 19/13191-5)

BRAGA, ZELIA VALENTE; DOS SANTOS, RICARDO FELICIANO; AMORIM, LILIAN; APPEZZATO-DA-GLORIA, BEATRIZ. Histopathological evidence of concomitant sexual and asexual reproduction of Elsinoe ampelina in grapevine under subtropical climate. PHYSIOLOGICAL AND MOLECULAR PLANT PATHOLOGY, v. 111, AUG 2020. (13/24003-9)