Tissue and biomechanics analysis of bones of rats fed high-fat diet: experimental study of the relationship between fat mass and bone loss

• Grant number: 14/10733-8
• Support Opportunities: Regular Research Grants
• Start date: August 01, 2014
• End date: January 31, 2017
• Field of knowledge: Engineering - Biomedical Engineering
• Principal Investigator: Antônio Carlos Shimano
• Grantee: Antônio Carlos Shimano
• Host Institution: Faculdade de Medicina de Ribeirão Preto (FMRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil
• Associated researchers: Alceu Afonso Jordão Junior ; Francisco José Albuquerque de Paula ; Sandra Yasuyo Fukada Alves

Abstract

The aim of this project is to evaluate the relationship between fat mass and bone tissue and bone loss. This project will be divided into two steps, being: 1) preliminary to investigate the effects of high fat diet in Wistar rats infant (21 days) during growth until sexual maturation stage. This step will consist of 2 groups: PAD (fed with standard diet) and HIP (fed with high fat diet) and 2) dependent on the results of Phase I, may be: "Study of the effects of early -fat diet on bone tissue of ovariectomized rats, and if this diet will be able to accelerate the deleterious effects on bone, caused by ovariectomy" or "Study of the effects of the return to standard diet after ovariectomy in rats in early intake of high-fat diet". These methodologies will consist of experimental groups with bone loss, using the ovariectomy model and with different exposure times and types of diet (high-fat or standard). Thus, to answer the questions raised in this study, the analyzes will be used: insulin tolerance, biochemical analysis of markers of formation/resorption (RANKL, OPG, osteocalcin and TRAP), liver tissue analysis, gene expression in the bone test (RT - PCR) of genes related to adiposity and osteogenesis, macroscopic analysis, bone densitometry, mechanical testing of trabecular and cortical bone, and microstructural analysis of micro- CT and scanning electron microscopy. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

REZENDE YANAGIHARA, G.; CARMINATI SHIMANO, R.; ATSUKO TIDA, J.; SUZUKI YAMANAKA, J.; YASUYO FUKADA, S.; MARDEGAN ISSA, J. P.; SHIMANO, A. C.; TAVARES, JOAO MANUEL R. S.. Influence of high-fat diet on bone tissue: An experimental study in growing rats. Journal of Nutrition Health & Aging, v. 21, n. 10, p. 1337-1343, DEC 2017. (15/26142-1, 14/10733-8, 14/25879-8)

SHIMANO, R. C.; YANAGIHARA, G. R.; MACEDO, A. P.; YAMANAKA, J. S.; SHIMANO, A. C.; TAVARES, J. M. R. S.; ISSA, J. P. M.. Effects of high-impact exercise on the physical properties of bones of ovariectomized rats fed to a high-protein diet. SCANDINAVIAN JOURNAL OF MEDICINE & SCIENCE IN SPORTS, v. 28, n. 5, p. 1523-1531, MAY 2018. (15/16194-4, 14/10733-8, 14/25879-8, 15/26142-1)

YAMANAKA, JESSICA SUZUKI; YANAGIHARA, GABRIELA REZENDE; CARLOS, BRUNA LEONEL; RAMOS, JUNIA; BRANCALEON, BRIGIDA BATISTA; MACEDO, ANA PAULA; MARDEGAN ISSA, JOAO PAULO; SHIMANO, ANTONIO CARLOS. A high-fat diet can affect bone healing in growing rats. JOURNAL OF BONE AND MINERAL METABOLISM, v. 36, n. 3, p. 255-263, MAY 2018. (14/10733-8)