Molecular characterization of Campylobacter jejuni and Campylobacter coli strains of diverse origins

• Grant number: 14/13029-0
• Support Opportunities: Regular Research Grants
• Start date: December 01, 2014
• End date: November 30, 2016
• Field of knowledge: Biological Sciences - Microbiology - Biology and Physiology of Microorganisms
• Principal Investigator: Juliana Pfrimer Falcão
• Grantee: Juliana Pfrimer Falcão
• Host Institution: Faculdade de Ciências Farmacêuticas de Ribeirão Preto (FCFRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil

Abstract

The genus Campylobacter belongs to the family Campylobacteraceae and currently comprises 25 species. The two most common species of clinical significance are Campylobacter jejuni and Campylobacter coli. The isolation of Campylobacter as a cause of gastroenteritis in humans in European and North American countries is very frequent. However, the isolation and the study of Campylobacter are not very common in Brazil, making it difficult to assess the importance of these species to as a cause of disease in humans and animals, as well as, to determine the impact of their presence in food and the environment. Furthermore, there are few studies that used molecular methods for typing C. jejuni and C. coli strains isolated in the country. The objectives of this project are to evaluate the genetic diversity, the pathogenic potential and the antimicrobial susceptibility of C. jejuni and C. coli strains isolated from humans, animals, food and environment in Brazil. A total of 126 C. jejuni strains isolated from humans (53), animals (40), food (29) and the environment (4), and 60 C. coli strains isolates from humans (10), animals (21), food (10) and the environment (19) during 16 years will be studied. The strains will be molecularly typed by pulsed-field gel electrophoresis (PFGE), sequencing of short variable region (SVR) of the flaA gene, analysis of the CRISPR locus (clustered regularly interspaced short palindromic repeats) by high resolution melting analysis (HRMA) and multilocus sequence typing (MLST). Moreover, it will be checked the antimicrobial susceptibility by the technique of minimal inhibitory concentration and the presence of some virulence markers by PCR. Taken together, the results to be obtained may contribute for a better understanding of the pathogenic potential, genotypic diversity and epidemiology of C. jejuni and C. coli strains isolated in Brazil. (AU)

Scientific publications

(16)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

GAMES, CAROLINA N.; SOUZA, ROBERTO A.; PASSAGLIA, JAQUELINE; DUQUE, SHEILA S.; MEDEIROS, MARTA I. C.; FALCAO, JULIANA P.. Genotyping of Campylobacter coli strains isolated in Brazil suggests possible contamination amongst environmental, human, animal and food sources. Journal of Medical Microbiology, v. 65, n. 1, p. 80-90, JAN 2016. (14/13029-0)

SERIBELLI, AMANDA AP.; FRAZAO, MILIANE R.; CAZENTINI MEDEIROS, MARTA I.; FALCAO, JULIANA P.. Molecular and phenotypic characterization of strains of Shigella sonnei isolated over 31 years suggests the circulation of two prevalent subtypes in Sao Paulo State, Brazil. Journal of Medical Microbiology, v. 65, n. 7, p. 666-677, JUL 2016. (14/13029-0)

FRAZAO, MILIANE RODRIGUES; CAO, GUOJIE; CAZENTINI MEDEIROS, MARTA INES; DUQUE, SHEILA DA SILVA; LEON, MARIA SANCHEZ; ALLARD, MARC WILLIAM; FALCAO, JULIANA PFRIMER. Draft Genome Sequences of 116 Campylobacter jejuni Strains Isolated from Humans, Animals, Food, and the Environment in Brazil. MICROBIOLOGY RESOURCE ANNOUNCEMENTS, v. 6, n. 16, APR 2018. (14/13029-0)

GOMES, CAROLINA N.; FRAZAO, MILIANE R.; PASSAGLIA, JAQUELINE; DUQUE, SHEILA S.; CAZENTINI MEDEIROS, I, MARTA; FALCAO, JULIANA P.. Molecular Epidemiology and Resistance Profile of Campylobacter jejuni and Campylobacter coli Strains Isolated from Different Sources in Brazil. MICROBIAL DRUG RESISTANCE, v. 26, n. 12, DEC 2019. (14/13029-0, 16/24716-3)

FRAZAO, MILIANE RODRIGUES; DE SOUZA, ROBERTO ANTONIO; CAZENTINI MEDEIROS, MARTA INES; DUQUE, SHEILA DA SILVA; CAO, GUOJIE; ALLARD, MARC WILLIAM; FALCAO, JULIANA PFRIMER. Molecular typing of Campylobacter jejuni strains: comparison among four different techniques. Brazilian Journal of Microbiology, v. 51, n. 2, DEC 2019. (14/13029-0)

CAMPIONI, FABIO; CAO, GUOJIE; KASTANIS, GEORGE; JANIES, DANIEL A.; MORATO BERGAMINI, ALZIRA MARIA; RODRIGUES, DALIA DOS PRAZERES; STONES, ROBERT; BROWN, ERIC; ALLARD, MARC W.; FALCAO, JULIANA PFRIMER. Changing of the Genomic Pattern of Salmonella Enteritidis Strains Isolated in Brazil Over a 48 year-period revealed by Whole Genome SNP Analyses. SCIENTIFIC REPORTS, v. 8, JUL 11 2018. (14/13029-0, 16/05817-3, 13/25191-3)

VILELA, F. P.; FRAZAO, M. R.; RODRIGUES, D. P.; COSTA, R. G.; CASAS, M. R. T.; FERNANDES, S. A.; FALCAO, J. P.; CAMPIONI, F.. Genetic diversity, anti-microbial resistance, plasmid profile and frequency of the Vi antigen in Salmonella Dublin strains isolated in Brazil. ZOONOSES AND PUBLIC HEALTH, v. 65, n. 1, p. E34-E43, FEB 2018. (14/13029-0, 15/10818-6, 13/25191-3)

FRAZAO, MILIANE RODRIGUES; CAO, GUOJIE; CAZENTINI MEDEIROS, MARTA INES; DUQUE, SHEILA DA SILVA; LEON, MARIA SANCHEZ; ALLARD, MARC WILLIAM; FALCAO, JULIANA PFRIMER. Draft Genome Sequences of 116 Campylobacter jejuni Strains Isolated from Humans, Animals, Food, and the Environment in Brazil. GENOME ANNOUNCEMENTS, v. 6, n. 16, p. 4-pg., 2018-04-01. (14/13029-0)

GONZALES, JULIA CUNHA; SERIBELLI, AMANDA APARECIDA; GOMES, CAROLINA NOGUEIRA; DOS PRAZERES RODRIGUES, DALIA; CAMPIONI, FABIO; PASSAGLIA, JAQUELINE; DA SILVA, PAULO; FALCAO, JULIANA PFRIMER. A high number of multidrug-resistant and predominant genetically related cluster ofShigella flexneristrains isolated over 34 years in Brazil. Brazilian Journal of Microbiology, v. 51, n. 4, p. 1563-1571, DEC 2020. (14/13029-0)

SERIBELLI, AMANDA APARECIDA; BALLABEN, ANELISE STELLA; FERREIRA, JOSEANE CRISTINA; CAZENTINI MEDEIROS, MARTA INES; COSTA DARINI, ANA LUCIA; FALCAO, JULIANA PFRIMER. IncU plasmid harbouring bla(CTX-M-8) in multidrug-resistant Shigella sonnei in Brazil. JOURNAL OF GLOBAL ANTIMICROBIAL RESISTANCE, v. 14, p. 99-100, SEP 2018. (15/23484-9, 14/13029-0, 14/14494-8)

SERIBELLI, AMANDA AP; FRAZAO, MILIANE R.; CAZENTINI MEDEIROS, MARTA I.; STEHLING, ELIANA G.; FALCAO, JULIANA P.. Molecular typing and occurrence of beta-lactam resistance genes of Shigella sonnei strains isolated from 1983 to 2014 in the Sao Paulo state of Brazil. MICROBIOLOGY AND IMMUNOLOGY, v. 61, n. 12, p. 547-553, DEC 2017. (14/13029-0)

FRAZAO, MILIANE RODRIGUES; CAZENTINI MEDEIROS, MARTA INES; DUQUE, SHEILA DA SILVA; FALCAO, JULIANA PFRIMER. Pathogenic potential and genotypic diversity of Campylobacter jejuni: a neglected food-borne pathogen in Brazil. Journal of Medical Microbiology, v. 66, n. 3, p. 350-359, MAR 2017. (14/13029-0)

VILELA, FELIPE PINHEIRO; GOMES, CAROLINA NOGUEIRA; PASSAGLIA, JAQUELINE; RODRIGUES, DALIA PRAZERES; COSTA, RENATA GARCIA; TIBA CASAS, MONIQUE RIBEIRO; FERNANDES, SUELI APARECIDA; FALCAO, JULIANA PFRIMER; CAMPIONI, FABIO. Genotypic Resistance to Quinolone and Tetracycline in Salmonella Dublin Strains Isolated from Humans and Animals in Brazil. MICROBIAL DRUG RESISTANCE, v. 25, n. 2, SEP 15 2018. (17/05756-7, 14/13029-0, 15/10818-6, 13/25191-3)

FRAZAO, MILIANE RODRIGUES; CAO, GUOJIE; MEDEIROS, MARTA INES CAZENTINI; DUQUE, SHEILA DA SILVA; ALLARD, MARC WILLIAM; FALCAO, JULIANA PFRIMER. Antimicrobial Resistance Profiles and Phylogenetic Analysis ofCampylobacter jejuniStrains Isolated in Brazil by Whole Genome Sequencing. MICROBIAL DRUG RESISTANCE, v. 27, n. 5, p. 660-669, MAY 1 2021. (14/13029-0, 16/24716-3, 18/06904-2, 19/19338-8)

SERIBELLI, AMANDA AP.; FRAZAO, MILIANE R.; CAZENTINI MEDEIROS, MARTA I.; FALCAO, JULIANA P.. Molecular and phenotypic characterization of strains of Shigella sonnei isolated over 31 years suggests the circulation of two prevalent subtypes in Sao Paulo State, Brazil. Journal of Medical Microbiology, v. 65, p. 12-pg., 2016-07-01. (14/13029-0)

GAMES, CAROLINA N.; SOUZA, ROBERTO A.; PASSAGLIA, JAQUELINE; DUQUE, SHEILA S.; MEDEIROS, MARTA I. C.; FALCAO, JULIANA P.. Genotyping of Campylobacter coli strains isolated in Brazil suggests possible contamination amongst environmental, human, animal and food sources. Journal of Medical Microbiology, v. 65, p. 11-pg., 2016-01-01. (14/13029-0)