Multi-User Equipment, previously approved in grant 2015/08166-5: stopped-flow

• Grant number: 15/10411-3
• Support Opportunities: Multi-user Equipment Program
• Start date: September 01, 2015
• End date: August 31, 2017
• Field of knowledge: Biological Sciences - Biophysics - Molecular Biophysics
• Principal Investigator: Iolanda Midea Cuccovia
• Grantee: Iolanda Midea Cuccovia
• Host Institution: Instituto de Química (IQ). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• Associated research grant: 13/08166-5 - Interfacial chemistry: drugs, peptides and ezymes interactions with membrane models, AP.TEM
• EMU web page: Página do Equipamento Multiusuário não informada
• Type of equipment: Processos Físicos - Manipulação de amostras - Fluxo interrompido (stopped flow); Caracterização de Materiais - Espectroscopia - Óptica (UV-Visível)
• Manufacturer: Fabricante não informado
• Model: Modelo não informado

Abstract

Using micelles and vesicles as models we will focus questions representing new frontiers in the study of interfacial properties using methods we have used for decades and deploying new techniques in collaboration with researchers from Brazil and abroad. Can we design (synthesize) self-aggregating surfactants that form vesicles capable of catalyzing and self-replicate? What is the role of lipid bilayer asymmetry in the properties and selective adsorption of ions or on the electrostatic properties of membranes? How can this selective binding of ions influence membrane / peptide interactions? How are ions are distributed within vesicles? What is the mechanism of association of the triflate ion in micelles and what does this tell us about ion association to interfaces? Using the dediazoniation reaction of substituted diazobenzenes can we design a nucleophilicity scale relevant to biological systems and determine ideality in solvent mixtures? How do mixed polymers aggregate and catalyze? How does human paraoxonase and asparaginase behave when interacting with membranes? What is the mechanism of interaction of BP100, a semisynthetic undecapeptide, with membranes? Can new imidazole derivatives and anti-inflammatory compounds be efficiently incorporated in liposomes? The answers to these questions, which are part of a line of thought of our group, will bring fundamental and applied contributions in the area of biomimetic systems. (AU)

Scientific publications

(6)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

PESKIN, V, ALEXANDER; MEOTTI, FLAVIA C.; KEAN, KELSEY M.; GOBL, CHRISTOPH; PEIXOTO, ALBERT SOUZA; PACE, PAUL E.; HORNE, CHRISTOPHER R.; HEATH, SARAH G.; CROWTHER, JENNIFER M.; DOBSON, RENWICK C. J.; et al. Modifying the resolving cysteine affects the structure and hydrogen peroxide reactivity of peroxiredoxin 2. Journal of Biological Chemistry, v. 296, JAN-JUN 2021. (15/10411-3, 13/07937-8, 18/14898-2)

LACERDA, CAROLINE DUTRA; CALEGARI ANDRADE, MARCOS FELIPE; PESSOA, PHILLIPE DE SANTANA; PRADO, FERNANDA MANSO; RODRIGUES PIRES, PAULO AUGUSTO; PINATTO-BOTELHO, MARCOS FELIPE; WODTKE, FELIPE; DOS SANTOS, ALCINDO APARECIDO; DIAS, LUIS GUSTAVO; LIMA, FILIPE DA SILVA; et al. Experimental mapping of a pH gradient from a positively charged micellar interface to bulk solution. COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS, v. 611, FEB 20 2021. (15/10411-3, 14/50983-3, 13/08166-5)

MARTINS, V. S.; TRIBONI, E. R.; BONILHA, J. B. S.; GONCALVES, L. M.; MORTARA, L.; CARVALHO, L. A. C.; MANDA, B. R.; LACERDA, C. D.; MEOTTI, F. C.; POLITI, M. J.; et al. Micellar effects and analytical applications of nitro substitution in 4-Nitro-N-alkyl-1,8-naphthalimide by cysteine derivatives. HELIYON, v. 6, n. 9, p. 14-pg., 2020-09-01. (18/15214-0, 18/14898-2, 16/00709-8, 18/19838-8, 11/18106-4, 15/10411-3, 13/02195-3)

LACERDA, C. D.; JULIANO, M. A.; LIRIA, C. W.; MACHINI, M. T.; PARK, P.; MATSUBARA, D. K.; BARZOTTO, D. R.; LIMA, F. S.; CHAIMOVICH, H.; CUCCOVIA, I. M.. Position matters in ester thiolysis by cysteine-containing peptides in micelles and vesicles. RESULTS IN CHEMISTRY, v. 6, p. 10-pg., 2023-12-01. (19/03023-8, 14/50983-3, 15/10411-3, 22/01825-2, 13/08166-5)

CARRETERO, GUSTAVO PENTEADO BATTESINI; SARAIVA, GREICE KELLE VIEGAS; RODRIGUES, MAGALI APARECIDA; KIYOTA, SUMIKA; BEMQUERER, MARCELO PORTO; CHAIMOVICH, HERNAN; CUCCOVIA, IOLANDA MIDEA. Naphthalimide-Containing BP100 Leads to Higher Model Membranes Interactions and Antimicrobial Activity. BIOMOLECULES, v. 11, n. 4, APR 2021. (18/15230-5, 14/50983-3, 13/08166-5, 15/10411-3)

ROCHA, LEONARDO SILVA; DA SILVA, BEATRIZ PEREIRA; CORREIA, THIAGO M. L.; DA SILVA, RAILMARA PEREIRA; MEIRELES, DIOGO DE ABREU; PEREIRA, RAFAEL; SOARES NETTO, LUIS EDUARDO; MEOTTI, FLAVIA CARLA; QUEIROZ, RAPHAEL FERREIRA. Peroxiredoxin AhpC1 protects Pseudomonas aeruginosa against the inflammatory oxidative burst and confers virulence. REDOX BIOLOGY, v. 46, OCT 2021. (15/21563-9, 13/07937-8, 15/10411-3, 18/14898-2, 19/26473-9)