Study of the involvement of mitochondrial activity in the super-response to caloric restriction in Caenorhabditis elegans mutants for lipl-5

• Grant number: 15/15526-3
• Support Opportunities: Regular Research Grants
• Start date: November 01, 2015
• End date: October 31, 2018
• Field of knowledge: Biological Sciences - Biochemistry - Metabolism and Bioenergetics
• Principal Investigator: Fernanda Marques da Cunha
• Grantee: Fernanda Marques da Cunha
• Host Institution: Escola Paulista de Medicina (EPM). Universidade Federal de São Paulo (UNIFESP). Campus São Paulo. São Paulo , SP, Brazil
• Associated researchers: Hugo Aguilaniu

Abstract

Mitochondria are dynamic organelles that integrate metabolic cues and modulate their own activity with the aim of regulating the production of energy and metabolic intermediates, as well as the induction of systemic responses. Together, these factors are crucial to the maintenance of homeostasis under stressful situations. Calorie restriction is a nutritional intervention that increases the longevity of a number of organisms, and part of its effect depends on the modulation of mitochondrial activity. The culture of C. elegans in axenic medium (bacteria-deprived medium) is most extreme protocol of calorie restriction capable of increasing the lifespan of adult worms. The main aim of the present project is to study the involvement of mitochondrial activity in the response induced by calorie restriction in C. elegans, especially in lipl-5 mutants. Those worms live 100% longer than wild type worms when subjected to bacteria deprivation, in spite of not mobilizing the totality of their stored lipid, as observed for wild type animals. The understanding of the modulation of mitochondrial function in response to extreme calorie restriction in a context where lipid stores are spared, will pave the way for the development of therapeutic strategies to promote health in people in whom the reduction of lipid stores is related to a worse prognosis, such as cancer patients and aged people with frailty. (AU)

Scientific publications

(5)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

AMIGO, IGNACIO; DA CUNHA, FERNANDA M.; FORNI, MARIA FERNANDA; GARCIA-NETO, WILSON; KAKIMOTO, PAMELA A.; LUEVANO-MARTINEZ, LUIS A.; MACEDO, FELIPE; MENEZES-FILHO, SERGIO L.; PELOGGIA, JULIA; KOWALTOWSKI, ALICIA J.. Mitochondrial form, function and signalling in aging. Biochemical Journal, v. 473, n. 20, p. 3421-3449, OCT 2016. (15/15526-3, 10/51906-1, 14/17270-3, 13/04919-9, 15/25776-7, 13/07937-8)

MACEDO, FELIPE; ROMANATTO, TALITA; DE ASSIS, CAROLINA GOMES; BUIS, ALEXIA; KOWALTOWSKI, ALICIA J.; AGUILANIU, HUGO; DA CUNHA, FERNANDA MARQUES. Lifespan-extending interventions enhance lipid-supported mitochondrial respiration inCaenorhabditis elegans. FASEB JOURNAL, v. 34, n. 8, JUL 2020. (13/07937-8, 15/15526-3)

MACEDO, FELIPE; MARTINS, GABRIEL LOUREIRO; LUEVANO-MARTINEZ, LUIS A.; VIANA, GUSTAVO MONTEIRO; RISKE, KARIN A.; INAGUE, ALEX; YOSHINAGA, MARCOS Y.; AGUILANIU, HUGO; MIYAMOTO, SAYURI; GLEZER, ISAIAS; et al. Lipase-like 5 enzyme controls mitochondrial activity in response to starvation in Caenorhabditis elegans. BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS, v. 1865, n. 2, FEB 2020. (16/12999-0, 13/07937-8, 15/15526-3, 16/21778-8)

AMIGO, IGNACIO; DA CUNHA, FERNANDA M.; FORNI, MARIA FERNANDA; GARCIA-NETO, WILSON; KAKIMOTO, PAMELA A.; LUEVANO-MARTINEZ, LUIS A.; MACEDO, FELIPE; MENEZES-FILHO, SERGIO L.; PELOGGIA, JULIA; KOWALTOWSKI, ALICIA J.. Mitochondrial form, function and signalling in aging. Biochemical Journal, v. 473, p. 29-pg., 2016-10-01. (13/07937-8, 13/04919-9, 15/15526-3, 14/17270-3, 10/51906-1, 15/25776-7)

MACEDO, FELIPE; DOS SANTOS, LUCAS SOUZA; GLEZER, ISAIAS; DA CUNHA, FEMANDA MARQUES. Brain Innate Immune Response in Diet-Induced Obesity as a Paradigm for Metabolic Influence on Inflammatory Signaling. FRONTIERS IN NEUROSCIENCE, v. 13, APR 24 2019. (15/15526-3, 13/07937-8, 18/18633-3)