Structural Studies and Characterization of Proteins by X-ray Crystallography and Nuclear Magnetic Resonance. Structural investigations and biophysics of molecular mechanisms of functional proteins.

• Grant number: 15/13765-0
• Support Opportunities: Regular Research Grants
• Start date: November 01, 2015
• End date: May 31, 2018
• Field of knowledge: Biological Sciences - Biophysics - Molecular Biophysics
• Principal Investigator: Raghuvir Krishnaswamy Arni
• Grantee: Raghuvir Krishnaswamy Arni
• Host Institution: Instituto de Biociências, Letras e Ciências Exatas (IBILCE). Universidade Estadual Paulista (UNESP). Campus de São José do Rio Preto. São José do Rio Preto , SP, Brazil

Abstract

This collaborative research project aims to express, characterize and determine the crystal structures of a number of enzymes. Crystallography provides accurate, static images of the three-dimensional structures of biological macromolecules at the atomic level that is unsurpassed by any other technique in its degree of detail and precision.Structural models help elucidate enzyme mechanisms and to comprehend complex biochemical processes inherent to life and biotechnology and to suggest structural modifications to inhibit the functioning of molecules that trigger or attenuate diseases and illnesses or to improve the performance of enzymes. The complimentary techniques of Nuclear Magnetic Resonance will be used to determine modes of binding and inhibition. (AU)

Scientific publications

(13)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

CORONADO, MONIKA APARECIDA; EBERLE, RAPHAEL JOSEF; BLEFFERT, NICOLE; FEUERSTEIN, SOPHIE; OLIVIER, DANILO SILVA; DE MORAES, FABIO ROGERIO; WILLBOLD, DIETER; ARNI, RAGHUVIR KRISHNASWAMY. Zika virus NS2B/NS3 proteinase: A new target for an old drug - Suramin a lead compound for NS2B/NS3 proteinase inhibition. Antiviral Research, v. 160, p. 118-125, DEC 2018. (16/08104-8, 15/13765-0, 15/18868-2, 09/53989-4, 16/12904-0)

CORONADO, MONIKA APARECIDA; OLIVIER, DANILO DA SILVA; EBERLE, RAPHAEL JOSEF; DO AMARAL, MARCOS SERROU; ARNI, RAGHUVIR KRISHNASWAMY. Modeling and molecular dynamics indicate that snake venom phospholipase B-like enzymes are Ntn-hydrolases. Toxicon, v. 153, p. 106-113, OCT 2018. (16/08104-8, 16/12904-0, 15/18868-2, 09/53989-4, 15/13765-0)

MARIUTTI, RICARDO B.; HERNANDEZ-GONZALEZ, JORGE E.; NASCIMENTO, ANDREY F. Z.; DE MORAIS, MARIANA A. B.; MURAKAMI, MARIO T.; CARARETO, CLAUDIA M. A.; ARNI, RAGHUVIR K.. A single P115Q mutation modulates specificity in the Corynebacterium pseudotuberculosis arginine repressor. BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS, v. 1864, n. 7, JUL 2020. (18/07977-3, 15/13765-0, 16/19995-0, 15/18868-2, 18/10736-8, 16/24587-9)

LOPES, TAIRINE ZARA; DE MORAES, FABIO ROGERIO; TEDESCO, ANTONIO CLAUDIO; ARNI, RAGHUVIR KRISHNASWAMY; RAHAL, PAULA; CALMON, MARILIA FREITAS. Berberine associated photodynamic therapy promotes autophagy and apoptosis via ROS generation in renal carcinoma cells (vol 123, 109794, 2020). BIOMEDICINE & PHARMACOTHERAPY, v. 125, p. 1-pg., 2020-05-01. (14/22198-0, 15/13765-0, 15/16660-5)

BHAT, SHREESHA K.; JOSHI, MANJUNATH B.; VASISHTA, SAMPARA; JAGADALE, RAJESH N.; BILIGIRI, SETLUR G.; CORONADO, MONIKA A.; ARNI, RAGHUVIR K.; SATYAMOORTHY, KAPAETTU. P-I metalloproteinases and L-amino acid oxidases from Bothrops species inhibit angiogenesis. Journal of Venomous Animals and Toxins including Tropical Diseases, v. 27, AUG 18 2021. (15/13765-0)

EBERLE, RAPHAEL J.; CORONADO, MONIKA A.; PEINADO, RAFAELA S.; DE MORAES, FABIO R.; OLIVIER, DANILO; DREYER, THIAGO; LOPES, DEBORA DE OLIVEIRA; ROSA DA LUZ, BRENDA SILVA; AZEVEDO, VASCO; ARNI, RAGHUVIR K.. The polyanions heparin and suramin impede binding of free adenine to a DNA glycosylase from C. pseudotuberculosis. International Journal of Biological Macromolecules, v. 125, p. 459-468, MAR 15 2019. (09/53989-4, 15/18868-2, 16/08104-8, 15/13765-0, 16/12904-0)

EBERLE, RAPHAEL J.; KAWAI, LIEGE A.; DE MORAES, FABIO R.; TASIC, LJUBICA; ARNI, RAGHUVIR K.; CORONADO, MONIKA A.. Biochemical and biophysical characterization of a mycoredoxin protein glutaredoxin A1 from Corynebacterium pseudotuberculosis. International Journal of Biological Macromolecules, v. 107, n. B, p. 1999-2007, FEB 2018. (16/08104-8, 15/13765-0, 15/18868-2, 09/53989-4, 16/12904-0)

DA SILVA, THAIS PEREIRA; DE CASTRO, FERNANDO JACOMINI; VUITIKA, LARISSA; POLLI, NAYANNE LOUISE COSTACURTA; ANTUNES, BRUNO CESAR; BOIA-FERREIRA, MARIANNA; MINOZZO, JOAO CARLOS; MARIUTTI, RICARDO BARROS; MATSUBARA, FERNANDO HITOMI; ARNI, RAGHUVIR KRISHNASWAMY; et al. Brown Spiders' Phospholipases-D with Potential Therapeutic Applications: Functional Assessment of Mutant Isoforms. BIOMEDICINES, v. 9, n. 3, MAR 2021. (15/18868-2, 15/13765-0)

EBERLE, RAPHAEL J.; KAWAI, LIEGE A.; DE MORAES, FABIO R.; OLIVIER, DANILO; DO AMARAL, MARCOS S.; TASIC, LJUBICA; AMI, RAGHUVIR K.; CORONADO, MONIKA A.. Inhibition of thioredoxin A1 from Corynebacterium pseudotuberculosis by polyanions and flavonoids. International Journal of Biological Macromolecules, v. 117, p. 1066-1073, OCT 1 2018. (09/53989-4, 15/18868-2, 16/08104-8, 15/13765-0, 16/12904-0)

GREMSKI, LUIZA HELENA; DA JUSTA, HANNA CAMARA; DA SILVA, THAIS PEREIRA; POLLI, NAYANNE LOUISE COSTACURTA; ANTUNES, BRUNO CESAR; MINOZZO, JOAO CARLOS; WILLE, ANA CAROLINA MARTINS; SENFF-RIBEIRO, ANDREA; ARNI, RAGHUVIR KRISHNASWAMY; VEIGA, SILVIO SANCHES. Forty Years of the Description of Brown Spider Venom Phospholipases-D. TOXINS, v. 12, n. 3, MAR 2020. (15/13765-0, 15/18868-2)

PEINADO, RAFAELA DOS S.; OLIVIER, DANILO S.; EBERLE, RAPHAEL J.; DE MORAES, FABIO R.; AMARAL, MARCOS S.; ARNI, RAGHUVIR K.; CORONADO, MONIKA A.. Binding studies of a putative C. pseudotuberculosis target protein from Vitamin B-12 Metabolism. SCIENTIFIC REPORTS, v. 9, APR 23 2019. (09/53989-4, 15/18868-2, 16/08104-8, 15/13765-0, 16/12904-0)

BHAT, SHREESHA K.; JOSHI, MANJUNATH B.; ULLAH, ANWAR; MASOOD, REHANA; BILIGIRI, SETLUR G.; ARNI, RAGHUVIR K.; SATYAMOORTHY, KAPAETTU. Serine proteinases from Bothrops snake venom activates PI3K/Akt mediated angiogenesis. Toxicon, v. 124, p. 63-72, DEC 15 2016. (15/13765-0)

EBERLE, RAPHAEL J.; KAWAI, LIEGE A.; DE MORAES, FABIO R.; TASIC, LJUBICA; ARNI, RAGHUVIR K.; CORONADO, MONIKA A.. Biochemical and biophysical characterization of a mycoredoxin protein glutaredoxin A1 from Corynebacterium pseudotuberculosis. International Journal of Biological Macromolecules, v. 107, p. 9-pg., 2018-02-01. (09/53989-4, 15/13765-0, 16/08104-8, 15/18868-2, 16/12904-0)