Physicochemical and structural properties of Ionic Liquids and drugs interacting with biologicaly relevant systems.

• Grant number: 15/15822-1
• Support Opportunities: Regular Research Grants
• Duration: December 01, 2015 - November 30, 2017
• Field of knowledge: Biological Sciences - Biophysics - Molecular Biophysics
• Principal Investigator: Leandro Ramos Souza Barbosa
• Grantee: Leandro Ramos Souza Barbosa
• Host Institution: Instituto de Física (IF). Universidade de São Paulo (USP). São Paulo , SP, Brazil

Abstract

This research project is divided into two parts; the first one will be centered in the study of the influence of ionic liquids (ILs) in biomimetic membrane systems and also in model proteins, whereas the second part (which will be developed concomitantly with the first), will be focused in the influence of drugs in the structure of liposomes (vesicles) used as carriers in different areas of health, with particular attention to leishmaniasis, the well-known Drug-delivery systems. The ILs, has attracted great attention from both the academic world, as well as the industrial one, due to its numerous applications. ILs are salts composed of an organic ion and a counter-ion that could be organic or inorganic and whose melting temperature is below 100 ° C, which means that these salts are in the liquid state at room temperature. Much of the interest in the study of ionic liquids is due to their low toxicity indicated by a low volatility. However little is known about their interaction with biological relevant systems such as proteins and lipid membranes. In this context we will study the influence of different ILs have the structure and function of proteins and model lipid membranes. In parallel we will also study the physicochemical changes induced by encapsulation of hydrophobic drugs in liposomes systems with the drug delivery purpose. These systems have vectoring capability, increased drug stability and the possibility of encapsulation of hydrophilic and lipophilic drugs inside. In particular we are interested in encapsulating drugs with potential use against negligible tropical diseases such as leishmaniasis. This stage of the project will be held in collaboration with prof. Dr. André Tempone, the Adolfo Lutz Institute, FMUSP. (AU)

Scientific publications

(21)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

SERAPHIM, V, THIAGO; NANO, NARDIN; CHEUNG, YIU WING SUNNY; ALUKSANASUWAN, SIRIPAT; COLLETI, CAROLINA; MAO, YU-QIAN; BHANDARI, VAIBHAV; YOUNG, GAVIN; HOLL, LARISSA; PHANSE, SADHNA; et al. ssembly principles of the human R2TP chaperone complex reveal the presence of R2T and R2P complexe. Structure, v. 30, n. 1, p. 156+, JAN 6 2022. (17/26131-5, 12/50161-8, 15/15822-1, 16/05019-0, 16/01603-9, 12/01953-9)

NANO, NARDIN; UGWU, FRANCISCA; SERAPHIM, V, THIAGO; LI, TANGZHI; AZER, GINA; ISAAC, METHVIN; PRAKESCH, MICHAEL; BARBOSA, LEANDRO R. S.; RAMOS, I, CARLOS H.; DATTI, ALESSANDRO; et al. Sorafenib as an Inhibitor of RUVBL2. BIOMOLECULES, v. 10, n. 4, APR 2020. (16/05019-0, 15/15822-1, 12/50161-8, 12/01953-9)

GONCALVES, CONRADO DE C.; PINHEIRO, GLAUCIA M. S.; DAHLSTROM, KATHE M.; SOUTO, DENIO E. P.; KUBOTA, LAURO T.; BARBOSA, LEANDRO R. S.; RAMOS, I, CARLOS H.. On the structure and function of Sorghum bicolor CHIP (carboxyl terminus of Hsc70-interacting protein): A link between chaperone and proteasome systems. Plant Science, v. 296, JUL 2020. (17/26158-0, 18/11948-9, 17/26058-6, 14/00076-0, 16/14228-1, 12/50161-8, 15/15822-1)

TIROLI-CEPEDA, ANA O.; SERAPHIM, THIAGO V.; PINHEIRO, GLAUCIA M. S.; SOUTO, DENIO E. P.; KUBOTA, LAURO T.; BORGES, JULIO C.; BARBOSA, LEANDRO R. S.; RAMOS, CARLOS H. I.. Studies on the effect of the J-domain on the substrate binding domain (SBD) of Hsp70 using a chimeric human J-SBD polypeptide. International Journal of Biological Macromolecules, v. 124, p. 111-120, MAR 1 2019. (14/07206-6, 17/07335-9, 15/15822-1, 12/50161-8)

WONG, KEITH S.; MABANGLO, MARK F.; SERAPHIM, THIAGO V.; MOLLICA, ANTONIO; MAO, YU-QIAN; RIZZOLO, KAMRAN; LEUNG, ELISA; MOUTAOUFIK, MOHAMED T.; HOELL, LARISSA; PHANSE, SADHNA; et al. Acyldepsipeptide Analogs Dysregulate Human Mitochondrial ClpP Protease Activity and Cause Apoptotic Cell Death. Cell Chemical Biology, v. 25, n. 8, p. 1017+, AUG 16 2018. (16/05019-0, 12/01953-9, 15/15822-1)

SILVA, NOELI S. M.; SERAPHIM, THIAGO V.; MINARI, KARINE; BARBOSA, LEANDRO R. S.; BORGES, JULIO C.. Comparative studies of the low-resolution structure of two p23 co-chaperones for Hsp90 identified in Plasmodium falciparum genome. International Journal of Biological Macromolecules, v. 108, p. 193-204, MAR 2018. (14/07206-6, 13/25646-0, 11/23110-0, 12/50161-8, 12/01953-9, 17/07335-9, 15/15822-1)

ADAO, REGINA; ZANPHORLIN, LETICIA M.; LIMA, TATIANI B.; SRIRANGANADANE, DEV; DAHLSTROM, KATHE M.; PINHEIRO, GLAUCIA M. S.; GOZZO, FABIO C.; BARBOSA, LEANDRO R. S.; RAMOS, CARLOS H. I.. Revealing the interaction mode of the highly flexible Sorghum bicolor Hsp70/Hsp90 organizing protein (Hop): A conserved carboxylate clamp confers high affinity binding to Hsp90. JOURNAL OF PROTEOMICS, v. 191, p. 11-pg., 2019-01-16. (14/17264-3, 12/50161-8, 15/15822-1)

LOTIERZO, MAYRA C. G.; CASADEI, BRUNA R.; DE CASTRO, RAPHAEL DIAS; MALHEIROS, BARBARA; BARBOSA, LEANDRO R. S.. Cubic-to-inverted micellar and the cubic-to-hexagonal-to-micellar transitions on phytantriol-based cubosomes induced by solvents. DRUG DELIVERY AND TRANSLATIONAL RESEARCH, v. 10, n. 6, p. 13-pg., 2020-08-11. (15/15822-1)

MABANGLO, MARK F.; LEUNG, ELISA; VAHIDI, SIAVASH; SERAPHIM, V, THIAGO; EGER, BRYAN T.; BRYSON, STEVE; BHANDARI, VAIBHAV; ZHOU, JIN LIN; MAO, YU-QIAN; RIZZOLO, KAMRAN; et al. ClpP protease activation results from the reorganization of the electrostatic interaction networks at the entrance pores. COMMUNICATIONS BIOLOGY, v. 2, NOV 13 2019. (16/05019-0, 15/15822-1, 12/50161-8, 12/01953-9)

GUIMARAES, MARINA DE SOUZA; MUSO CACHUMBA, JORGE JAVIER; BUENO, CECILIA ZORZI; TORRES-OBREQUE, KARIN MARIANA; RUIZ LARA, GRACE VERONICA; MONTEIRO, GISELE; SOUZA BARBOSA, LEANDRO RAMOS; PESSOA JR, ADALBERTO; RANGEL-YAGUI, CARLOTA DE OLIVEIRA. Peg-Grafted Liposomes for L-Asparaginase Encapsulation. PHARMACEUTICS, v. 14, n. 9, p. 18-pg., 2022-09-01. (18/15104-0, 13/08617-7, 15/15822-1, 14/10456-4)

ADAO, REGINA; ZANPHORLIN, LETICIA M.; LIMA, TATIANI B.; SRIRANGANADANE, DEV; DAHLSTROM, KATHE M.; PINHEIRO, GLAUCIA M. S.; GOZZO, FABIO C.; BARBOSA, LEANDRO R. S.; RAMOS, CARLOS H. I.. Revealing the interaction mode of the highly flexible Sorghum bicolor Hsp70/Hsp90 organizing protein (Hop): A conserved carboxylate clamp confers high affinity binding to Hsp90. JOURNAL OF PROTEOMICS, v. 191, n. SI, p. 191-201, JAN 16 2019. (14/17264-3, 15/15822-1, 12/50161-8)

LOTIERZO, MAYRA C. G.; CASADEI, BRUNA R.; DE CASTRO, RAPHAEL DIAS; MALHEIROS, BARBARA; BARBOSA, LEANDRO R. S.. Cubic-to-inverted micellar and the cubic-to-hexagonal-to-micellar transitions on phytantriol-based cubosomes induced by solvents. DRUG DELIVERY AND TRANSLATIONAL RESEARCH, v. 10, n. 6, SI, AUG 2020. (15/15822-1)

CARDUCCI, FEDERICA; CASADEI, BRUNA RENATA; MARIANI, PAOLO; BARBOSA, LEANDRO RAMOS SOUZA. X-Ray Characterization of Pharmaceutical and Cosmetic Lipidic Nanoparticles for Cutaneous Application. CURRENT PHARMACEUTICAL DESIGN, v. 25, n. 21, p. 2364-2374, 2019. (15/15822-1)

QUEL, NATALIA G.; RODRIGUES, LUIZ FERNANDO DE C.; ARAGAO, ANNELIZE Z. B.; PINHEIRO, GLAUCIA M. S.; CAMACHO, RAFAEL P.; SOUTO, DENIO E. P.; KUBOTA, LAURO T.; BARBOSA, LEANDRO R. S.; RAMOS, CARLOS H. I.. Insights into the structure and function of the C-terminus of SGTs (small glutamine-rich TPR-containing proteins): A study of the Aedes aegypti homolog. Biochimie, v. 187, p. 131-143, AUG 2021. (16/05019-0, 12/01953-9, 14/25967-4, 17/26131-5, 15/15822-1)

QUEL, NATALIA G.; PINHEIRO, GLAUCIA M. S.; RODRIGUES, LUIZ FERNANDO DE C.; BARBOSA, LEANDRO R. S.; HOURY, WALID A.; RAMOS, I, CARLOS H.. Heat shock protein 90 kDa (Hsp90) from Aedes aegypti has an open conformation and is expressed under heat stress. International Journal of Biological Macromolecules, v. 156, p. 522-530, AUG 1 2020. (17/26131-5, 16/05019-0, 15/15822-1, 14/25967-4, 12/01953-9)

FEITOSA, VALKER ARAUJO; DE ALMEIDA, VINICIUS CORDEIRO; MALHEIROS, BARBARA; DE CASTRO, RAPHAEL DIAS; SOUZA BARBOSA, LEANDRO RAMOS; PEREIRA CERIZE, NATALIA NETO; RANGEL-YAGUI, CARLOTA DE OLIVEIRA. Polymeric micelles of pluronic F127 reduce hemolytic potential of amphiphilic drugs. COLLOIDS AND SURFACES B-BIOINTERFACES, v. 180, p. 177-185, AUG 1 2019. (15/15822-1, 14/01983-0)

DA COSTA-SILVA, THAIS ALVES; GALISTEO, JR., ANDRES JIMENEZ; LAULETTA LINDOSO, JOSE ANGELO; BARBOSA, LEANDRO R. S.; TEMPONE, ANDRE GUSTAVO. Nanoliposomal Buparvaquone Immunomodulates Leishmania infantum-Infected Macrophages and Is Highly Effective in a Murine Model. Antimicrobial Agents and Chemotherapy, v. 61, n. 4, APR 2017. (15/15822-1, 15/23403-9, 13/07275-5, 13/50228-8)

MALHEIROS, BARBARA; DE CASTRO, RAPHAEL DIAS; LOTIERZO, MAYRA C.; CASADEI, BRUNA R.; MARIANI, PAOLO; BARBOSA, LEANDRO R. S.. Influence of hexadecylphosphocholine (Miltefosine) in phytantriol-based cubosomes: A structural investigation. COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS, v. 632, JAN 2 2022. (15/15822-1)

ROMANELLI, MAIARA MARIA; DA COSTA-SILVA, THAIS ALVES; CUNHA-JUNIOR, EDEZIO; DIAS FERREIRA, DAIANE; GUERRA, JULIANA M.; GALISTEO, JR., ANDRES JIMENEZ; PINTO, ERIKA GRACIELLE; BARBOSA, LEANDRO R. S.; TORRES-SANTOS, EDUARDO CAIO; TEMPONE, ANDRE GUSTAVO. Sertraline Delivered in Phosphatidylserine Liposomes Is Effective in an Experimental Model of Visceral Leishmaniasis. FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY, v. 9, OCT 29 2019. (18/10279-6, 15/15822-1, 13/07275-5, 15/23403-9)

MALHEIROS, BARBARA; DE CASTRO, RAPHAEL DIAS; LOTIERZO, MAYRA C. G.; CASADEI, BRUNA R.; BARBOSA, LEANDRO R. S.. Design and manufacturing of monodisperse and malleable phytantriol-based cubosomes for drug delivery applications. JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY, v. 61, FEB 2021. (15/15822-1)

MELO SILVA, NOELI SOARES; DE CAMARGO RODRIGUES, LUIZ FERNANDO; DORES-SILVA, PAULO ROBERTO; MONTANARI, CARLOS ALBERTO; INACIO RAMOS, CARLOS HENRIQUE; SOUZA BARBOSA, LEANDRO RAMOS; BORGES, JULIO CESAR. Structural, thermodynamic and functional studies of human 71 kDa heat shock cognate protein (HSPA8/hHsc70). BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS, v. 1869, n. 12, DEC 2021. (17/07335-9, 11/23110-0, 17/26131-5, 12/50161-8, 15/15822-1, 14/07206-6, 14/16646-0)