Semaphorins and neuropilins expression in salivary gland tumors

• Grant number: 16/07139-2
• Support Opportunities: Regular Research Grants - Publications - Scientific article
• Start date: June 01, 2016
• End date: November 30, 2016
• Field of knowledge: Health Sciences - Dentistry
• Principal Investigator: Pablo Agustin Vargas
• Grantee: Pablo Agustin Vargas
• Host Institution: Faculdade de Odontologia de Piracicaba (FOP). Universidade Estadual de Campinas (UNICAMP). Piracicaba , SP, Brazil

Abstract

Backgroud: Salivary gland tumors (SGT) account for 3% to 10% of all head and neck neoplasms and little is known about their angiogenic properties. Despite semaphorins and neuropilins have been demonstrated to be prognostic determinants in many human cancers, they remain to be investigated in SGT. Therefore, the objective of this study is to analyze the clinical significance of the expression of class 3 semaphorins A (Sema3A) and B (Sema3B) and neuropilins-1 (Np-1) and -2 (Np-2), in SGT.Methods: Two hundred and forty eight SGT were organized in tissue microarray paraffin blocks and expression of CD34, Sema3A, Sema3B, Np-1 and Np-2 was determined through immunohistochemistry. The immunoreactions were quantified using digital algorithms and the results correlated with clinicopathological parameters. Results: Malignant tumors had an increased vascular density than their benign counterparts and their increased vascular area significantly correlated with recurrences (p < 0.05). Patients older than 40 years and presence of recurrences determined an inferior survival rate (p = 0.0057 and p = 0.0303, respectively). In normal salivary glands Np-1 and Np-2 expression was restricted to ductal cells, whereas Sema3A and Sema3B were positive in the serous acinar compartment. Tumors were positive for all markers and the co-expression of Np-1/Np-2 significantly correlated with presence of paresthesia and advanced stages of the tumors (p = 0.01 and p = 0.04, respectively). Conclusion: Sema3A, Sema3B, Np-1 and Np-2 may be involved in the pathogenesis of SGT, but their expression did not present a statistically significant prognostic potential in the current study. (AU)