Biomolecule damage in neural cellular system in metalic and redox inbalance

• Grant number: 16/09652-9
• Support Opportunities: Regular Research Grants
• Start date: July 01, 2016
• End date: June 30, 2018
• Field of knowledge: Physical Sciences and Mathematics - Chemistry - Inorganic Chemistry
• Principal Investigator: Giselle Cerchiaro
• Grantee: Giselle Cerchiaro
• Host Institution: Centro de Ciências Naturais e Humanas (CCNH). Universidade Federal do ABC (UFABC). Ministério da Educação (Brasil). Santo André , SP, Brazil

Abstract

Much is known about the oxidation of biomolecules and its relationship with reactive oxygen species (ROS) and intracellular redox imbalance systems. However little is known regarding the transition metals present in trace amounts (such as copper, iron and zinc) in engagement with this type of oxidation mechanism. Our research group has actively contributed to the elucidation of the role of metals in biological environment, but this seemingly simple question in neurodegeneration and redox imbalance is still unclear from the molecular point of view. This research project seek elucidation of some of these issues, noting the processes of homeostasis of copper and zinc metal (constituents of SOD1 enzyme) as well as compared to treatment with chelating agents and proteins involved in neurodegeneration (±²-amyloid), and the ability of igniting their interactions in oxidative damage, DNA damage and cell death, as well as signaling mechanisms of these processes. Thus, it is intended to provide a molecular mechanism for the relation between zinc, copper, and cellular ROS neurodegeneration.The project's main objective is the evaluation of how the redox imbalance and metal imbalance (specially copper and zinc) can cause damage to various biomolecules in neuronal cell models (hippocampal and motor neuron) submitted to ROS and neurodegeneration. (AU)

Scientific publications

(10)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

NUNES, EMILENE A.; MANIERI, TANIA M.; MATIAS, ANDREZA C.; BERTUCHI, FERNANDA R.; DA SILVA, DANIELA A.; LAGO, LARISSA; SATO, ROSELI H.; CERCHIARO, GISELLE. Protective effects of neocuproine copper chelator against oxidative damage in NSC34 cells. MUTATION RESEARCH-GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS, v. 836, n. B, SI, p. 62-71, DEC 2018. (16/09652-9)

TORRES, MARCELO D. T.; ANDRADE, GISLAINE P.; SATO, ROSELI H.; PEDRON, CIBELE N.; MANIERI, TANIA M.; CERCHIARO, GISELLE; RIBEIRO, ANDERSON O.; DE LA FUENTE-NUNEZ, CESAR; OLIVEIRA, JR., VANI X.. Natural and redesigned wasp venom peptides with selective antitumoral activity. Beilstein Journal of Organic Chemistry, v. 14, p. 1693-1703, JUL 6 2018. (14/18527-8, 16/09652-9, 14/12938-6, 14/04507-5)

RAMOS, LUIZ D.; CERCHIARO, GISELLE; MORELLI FRIN, KARINA P.. Rhenium(I) polypyridine complexes coordinated to an ethyl-isonicotinate ligand: Luminescence and in vitro anti-cancer studies. Inorganica Chimica Acta, v. 501, FEB 1 2020. (17/18063-0, 16/09652-9)

LOURENCO, I. M.; PELEGRINO, M. T.; PIERETTI, J. C.; ANDRADE, G. P.; CERCHIARO, G.; SEABRA, A. B.; IOP. Synthesis, characterization and cytotoxicity of chitosan-coated Fe3O4 nanoparticles functionalized with ascorbic acid for biomedical applications. WAKE CONFERENCE 2021, v. 1323, p. 10-pg., 2019-01-01. (18/02832-7, 16/10347-6, 18/13761-3, 16/09652-9, 17/05029-8)

RAMOS, LUIZ D.; DE MACEDO, LEONARDO H.; GOBO, NICHOLAS R. S.; DE OLIVEIRA, KLEBER T.; CERCHIARO, GISELLE; MORELLI FRIN, KARINA P.. Understanding the photophysical properties of rhenium(I) compounds coordinated to 4,7-diamine-1,10-phenanthroline: synthetic, luminescence and biological studies. DALTON TRANSACTIONS, v. 49, n. 45, p. 16154-16165, DEC 7 2020. (18/14152-0, 18/00106-7, 17/18063-0, 16/09652-9)

PEDRON, CIBELE NICOLASKI; ANDRADE, GISLAINE PATRICIA; SATO, ROSELI HIROMI; TOROSSIAN TORRES, MARCELO DER; CERCHIARO, GISELLE; RIBEIRO, ANDERSON ORZARI; OLIVEIRA, JR., VANI XAVIER. Anticancer activity of VmCT1 analogs against MCF-7 cells. CHEMICAL BIOLOGY & DRUG DESIGN, v. 91, n. 2, p. 588-596, FEB 2018. (14/18527-8, 16/09652-9, 14/12938-6, 14/04507-5)

SANDANIELO MARQUES, CAROLINE MARTINS; NUNES, EMILENE ARUSIEVICZ; LAGO, LARISSA; PEDRON, CIBELE NICOLASKI; MANIERI, TANIA MARIA; SATO, ROSELI HIROMI; OLIVEIRA JUNIOR, VANI XAVIER; CERCHIARO, GISELLE. Generation of Advanced Glycation End-Products (AGEs) by glycoxidation mediated by copper and ROS in a human serum albumin (HSA) model peptide: reaction mechanism and damage in motor neuron cells. MUTATION RESEARCH-GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS, v. 824, p. 42-51, DEC 2017. (16/09652-9)

VALLE, ELIANA MAIRA A.; MALTAROLLO, VINICIUS GONCALVES; ALMEIDA, MICHELL O.; HONORIO, KATHIA MARIA; DOS SANTOS, MAURO COELHO; CERCHIARO, GISELLE. Time dependent-density functional theory (TD-DFT) and experimental studies of UV-Visible spectra and cyclic voltammetry for Cu(II) complex with Et2DTC. Journal of Molecular Structure, v. 1157, p. 463-468, APR 5 2018. (16/09652-9, 15/10314-8)

LAGO, LARISSA; NUNES, EMILENE A.; VIGATO, ARYANE A.; SOUZA, VANESSA C. O.; BARBOSA, JR., FERNANDO; SATO, JOAO R.; BATISTA, BRUNO L.; CERCHIARO, GISELLE. Flow of essential elements in subcellular fractions during oxidative stress. BIOMETALS, v. 30, n. 1, p. 83-96, FEB 2017. (16/09652-9)

NUNES, EMILENE A.; MANIERI, TANIA M.; MATIAS, ANDREZA C.; BERTUCHI, FERNANDA R.; DA SILVA, DANIELA A.; LAGO, LARISSA; SATO, ROSELI H.; CERCHIARO, GISELLE. Protective effects of neocuproine copper chelator against oxidative damage in NSC34 cells. MUTATION RESEARCH-GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS, v. 836, p. 10-pg., 2018-12-01. (16/09652-9)