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PneumoPhageColor - development of a colorimetric biodetection kit for Pseudomonas aeruginosa based on phage particles

Grant number: 16/08884-3
Support type:Regular Research Grants
Duration: December 01, 2016 - November 30, 2018
Field of knowledge:Biological Sciences - Microbiology
Principal Investigator:Vitor Manuel Cardoso Figueiredo Balcão
Grantee:Vitor Manuel Cardoso Figueiredo Balcão
Home Institution: Pró-Reitoria Acadêmica. Universidade de Sorocaba (UNISO). Sorocaba , SP, Brazil
Assoc. researchers:Fernando de Sá Del Fiol ; Marco Vinícius Chaud ; Marta Maria Duarte Carvalho Vila ; Matthieu Tubino ; Renata de Lima


Infectious diseases kill between 17-20 million people a year worldwide, and about 10 million people acquire hospital infections (HI) and, from this universe, almost 300,000 die. HIs increase both the length of permanence in the hospital and treatment costs, besides contributing significantly to the morbidity and mortality in hospitalized patients. In Brazil, the exact prevalence of HIs is still unknown, however, a national survey conducted by the Ministry of Health revealed, among several institutions evaluated, a rate of nosocomial infections ranging from 13% to 15%. The rate of HIs in Brazil presents high values and is considered almost the double when compared to other countries. Thus, hospital acquired infections represents a major challenge to be faced by the government and society for the implementation of prevention and control of infection in hospitals. Among the microorganisms that cause HIs one can highlight the multidrug-resistant Pseudomonas aeruginosa, which represents a major threat to patients in health care services. Data from 2007 points to P. aeruginosa as the most frequently isolated pathogen in patients with nosocomial pneumonia, a common cause of urinary tract infections, surgical wound and burn infections, being also present in bloodstream infections. The bacterium P. aeruginosa has been highlighted in the social media in Brazil about seven years ago, following the death of the 20 year-old model Mariana Bridi, on January 24th 2009, at Dorio Silva Hospital in the city of Serra (ES). According to a spokesperson of the state government, the death was due to complications from a generalized serious infection, originated from a urinary tract infection. In recent years, nosocomial infections caused by multiresistant Gram-negative microorganisms has increased consistently in Brazilian hospitals, and the bacterium P. aeruginosa was responsible for 10% of all nosocomial infections. It is intended that PneumoPhageColor will be a rapid detection (> 2 hours <5h) and low-cost kit for the high precision biodetection of P. aeruginosa, based on the use of a hydrogel sensitive to color changes, fixed on a support material, containing phage particles (or a cocktail of phage particles) specific and strictly lytic against P. aeruginosa. The proposed concept is not new, existing from about 3.5 billion years, having been initiated when bacteria and bacteriophages established a predator-prey balance. Bacteriophages are the most abundant organisms on our planet (are estimated at more than 10^31 particles) being completely safe for all organisms including humans, except for their target bacterial hosts. The idea embodied in PneumoPhageColor is unconventional and a highly creative approach, since it will join the solution (phage) with the problem (bacteria) in order to detect the latter. One intends to establish the proof-of-concept using a bacteriophage and a collection strain of P. aeruginosa. This research project will investigate the potential for the structural and functional stabilization of a lytic phage (or cocktail of lytic phage particles) with broad-spectrum, capable of infecting P. aeruginosa, within a hydrogel with chromogenic characteristics. Specific reagents will be incorporated into the optimized hydrogel in varying concentrations, in order to produce signal (color) if it is present in the gel a certain intracytoplasmic enzyme, released by phage lysis should bacterial cells of P. aeruginosa become into contact with the hydrogel. The intensity of the color produced in the hydrogel will give an idea of the bacterial load present, and this intensity will be measured by an optical sensor coupled to a signal transducer. (AU)

Scientific publications (6)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
LIMA, RENATA; DEL FIOL, FERNANDO SA; BALCAO, VICTOR M. Prospects for the Use of New Technologies to Combat Multidrug-Resistant Bacteria. FRONTIERS IN PHARMACOLOGY, v. 10, JUN 21 2019. Web of Science Citations: 1.
FAVARO, LAURA I. L.; BALCAO, VICTOR M.; ROCHA, LILIAM K. H.; SILVA, ERICA C.; OLIVEIRA JR, JOSE M.; VILA, MARTA M. D. C.; TUBINO, MATTHIEU. Physicochemical Characterization of a Crude Anthocyanin Extract from the Fruits of Jussara (Euterpe edulis Martius): Potential for Food and Pharmaceutical Applications. Journal of the Brazilian Chemical Society, v. 29, n. 10, p. 2072-2088, OCT 2018. Web of Science Citations: 3.
HARADA, LILIAM K.; PEREIRA, JORGE F. B.; CAMPOS, WELIDA F.; SILVA, ERICA C.; MOUTINHO, CARLA G.; VILA, MARTA M. D. C.; OLIVEIRA, JR., JOSE M.; TEIXEIRA, JOSE A.; BALCAO, VICTOR M.; TUBINO, MATTHIEU. Insights into Protein-Ionic Liquid Interactions Aiming at Macromolecule Delivery Systems. Journal of the Brazilian Chemical Society, v. 29, n. 10, p. 1983-1998, OCT 2018. Web of Science Citations: 2.
RIOS, ALESSANDRA C.; VILA, MARTA M. D. C.; LIMA, RENATA; DEL FIOL, FERNANDO S.; TUBINO, MATTHIEU; TEIXEIRA, JOSE A.; BALCAO, VICTOR M. Structural and functional stabilization of bacteriophage particles within the aqueous core of a W/O/W multiple emulsion: A potential biotherapeutic system for the inhalational treatment of bacterial pneumonia. Process Biochemistry, v. 64, p. 177-192, JAN 2018. Web of Science Citations: 4.
HARADA, LILIAM K.; SILVA, ERICA C.; CAMPOS, WELIDA F.; DEL FIOL, FERNANDO S.; VILA, MARTA; DABROWSKA, KRYSTYNA; KRYLOV, VICTOR N.; BALCAO, VICTOR M. Biotechnological applications of bacteriophages: State of the art. MICROBIOLOGICAL RESEARCH, v. 212, p. 38-58, 2018. Web of Science Citations: 13.
ROCHA, LILIAM K. H.; FAVARO, LAURA I. L.; RIOS, ALESSANDRA C.; SILVA, ERICA C.; SILVA, WELIDA F.; STIGLIANI, TATIANE P.; GUNGER, MARIANA; LIMA, RENATA; OLIVEIRA, JR., JOSE M.; ARANHA, NORBERTO; TUBINO, MATTHIEU; VILA, MARTA M. D. C.; BALCAO, VICTOR M. Sericin from Bombyx mori cocoons. Part I: Extraction and physicochemical-biological characterization for biopharmaceutical applications. Process Biochemistry, v. 61, p. 163-177, OCT 2017. Web of Science Citations: 8.

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