Bioprospecting for natural compounds and bioactive derivatives with potent anticancer activity: in vitro/in vivo assays and investigation into the mechanism of action.

• Grant number: 16/06137-6
• Support Opportunities: Regular Research Grants
• Start date: November 01, 2016
• End date: August 31, 2019
• Field of knowledge: Biological Sciences - Biology
• Principal Investigator: Giovanna Barbarini Longato
• Grantee: Giovanna Barbarini Longato
• Host Institution: Universidade São Francisco (USF). Campus Bragança Paulista. Bragança Paulista , SP, Brazil
• Associated researchers: Mary Ann Foglio ; Patrícia de Oliveira Carvalho ; Wagner Vilegas

Abstract

This project aims to accomplish the bioprospecting for natural compounds and bioactive derivatives with anticancer activity. The compounds to be tested (plant extracts, active fractions, isolated compounds and bioactive compounds) are from different research centers within and outside the institution. Initially, 25 isolated compounds and bioactive compounds provided by Faculty of Pharmaceutical Science - UNICAMP, Biodiversity and Sustainability Laboratory - UNESP and Multidisciplinary Research Laboratory - USF will be evaluated for their potential antiproliferative activity in cultured human tumor. Furthermore, they will be evaluated for the ability to prevent cell invasion and colony formation. The most promising compounds will be targeted for in vivo (Ehrlich solid tumor) testing and their mechanism of action will be evaluated by western blotting and flow cytometry, key techniques to investigate cell death process. The approaches described herein are fundamental to identify potential antitumor compounds, candidates for future clinical trials, aiming at developing a new anticancer drug originated from Brazilian biodiversity. (AU)

Scientific publications

(9)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

FRANCO, YOLLANDA E. M.; DE LIMA, CAROLINA A.; ROSA, MARCELA N.; SILVA, VIVIANE A. O.; REIS, RUI M.; PRIOLLI, DENISE G.; CARVALHO, PATRICIA O.; DO NASCIMENTO, JESSYANE R.; DA ROCHA, CLAUDIA Q.; LONGATO, GIOVANNA B.. Investigation of U-251 cell death triggered by flavonoid luteolin: towards a better understanding on its anticancer property against glioblastomas. NATURAL PRODUCT RESEARCH, FEB 2020. (16/02345-3, 16/06137-6)

AFONSO DE LIMA, CAROLINA; DE SOUZA BUENO, IAN LUCAS; NUNES SIQUEIRA VASCONCELOS, STANLEY; SCIANI, JULIANA MOZER; RUIZ, ANA LUCIA TASCA GOIS; FOGLIO, MARY ANN; DE CARVALHO, JOAO ERNESTO; BARBARINI LONGATO, GIOVANNA. Reversal of Ovarian Cancer Cell Lines Multidrug Resistance Phenotype by the Association of Apiole with Chemotherapies. PHARMACEUTICALS, v. 13, n. 10, OCT 2020. (18/09475-5, 16/06137-6)

SENEME, ELISA FREDERICO; DOS SANTOS, DAIANE CARLA; DE LIMA, CAROLINA AFONSO; MACCARI ZELIOLI, ICARO AUGUSTO; SCIANI, JULIANA MOZER; LONGATO, GIOVANNA BARBARINI. Effects of Myristicin in Association with Chemotherapies on the Reversal of the Multidrug Resistance (MDR) Mechanism in Cancer. PHARMACEUTICALS, v. 15, n. 10, p. 12-pg., 2022-10-01. (16/06137-6)

FERNANDES, YAN M. L.; MATOS, JULIA V. S.; LIMA, CAROLINA A.; TARDINI, ANGELA M.; VIERA, FLAVIO A. P.; MAIA, JOSE G. S.; MONTEIRO, ODAIR S.; LONGATO, GIOVANNA B.; ROCHA, CLAUDIA Q.. Essential Oils Obtained from Aerial Eugenia punicifolia Parts: Chemical Composition and Antiproliferative Potential Evidenced through Cell Cycle Arrest. Journal of the Brazilian Chemical Society, v. 32, n. 7, p. 1381-1390, JUL 2021. (18/19436-7, 16/06137-6)

ARRUDA, GIAN LUCAS M.; VIGERELLI, HUGO; BUFALO, MICHELLE C.; LONGATO, GIOVANNA B.; VELOSO, RODINEI V.; ZAMBELLI, VANESSA O.; PICOLO, GISELE; CURY, YARA; MORANDINI, ANDRE C.; MARQUES, ANTONIO CARLOS; et al. Box Jellyfish (Cnidaria, Cubozoa) Extract Increases Neuron's Connection: A Possible Neuroprotector Effect. BIOMED RESEARCH INTERNATIONAL, v. 2021, MAR 5 2021. (15/21007-9, 15/50040-4, 16/06137-6, 19/19929-6, 13/07467-1, 11/50242-5)

FRANCO, YOLLANDA E. M.; DE LIMA, CAROLINA A.; ROSA, MARCELA N.; SILVA, VIVIANE A. O.; REIS, RUI M.; PRIOLLI, DENISE G.; CARVALHO, PATRICIA O.; DO NASCIMENTO, JESSYANE R.; DA ROCHA, CLAUDIA Q.; LONGATO, GIOVANNA B.. Investigation of U-251 cell death triggered by flavonoid luteolin: towards a better understanding on its anticancer property against glioblastomas. NATURAL PRODUCT RESEARCH, v. 35, n. 22, p. 7-pg., 2020-02-20. (16/06137-6, 16/02345-3)

FRANCO, YOLLANDA E. M.; OKUBO, MARCIA Y.; TORRE, ADRIANA D.; PAIVA, PAULA P.; ROSA, MARCELA N.; SILVA, VIVIANE A. O.; REIS, RUI M.; RUIZ, ANA L. T. G.; IMAMURA, PAULO M.; DE CARVALHO, JOAO E.; et al. Coronarin D Induces Apoptotic Cell Death and Cell Cycle Arrest in Human Glioblastoma Cell Line. Molecules, v. 24, n. 24, DEC 2019. (16/06137-6, 14/06636-7)

BANZATO, THAIS P.; GUBIANI, JULIANA R.; BERNARDI, DARLON I.; NOGUEIRA, CLAUDIO R.; MONTEIRO, AFIF F.; JULIANO, FERNANDA F.; DE ALENCAR, SEVERINO M.; PILLI, RONALDO A.; DE LIMA, CAROLINA A.; LONGATO, GIOVANNA B.; et al. Antiproliferative Flavanoid Dimers Isolated from Brazilian Red Propolis. Journal of Natural Products, v. 83, n. 6, p. 1784-1793, JUN 26 2020. (13/24416-1, 17/06014-4, 16/06137-6, 13/50228-8)

DE LIMA, CAROLINA A.; CUBERO, MAYRA C. Z.; FRANCO, YOLLANDA E. M.; RODRIGUES, CARLA D. P.; DO NASCIMENTO, JESSYANE R.; VENDRAMINI-COSTA, DEBORA B.; SCIANI, JULIANA M.; DA ROCHA, CLAUDIA Q.; LONGATO, GIOVANNA B.. Antiproliferative Activity of Two Unusual Dimeric Flavonoids, Brachydin E and Brachydin F, Isolated from Fridericia platyphylla (Cham.) LGLohmann: In Vitro and Molecular Docking Evaluation. BIOMED RESEARCH INTERNATIONAL, v. 2022, p. 12-pg., 2022-02-11. (17/01127-5, 16/06137-6)