Metagenomic profiles of microbial comunities associated with sugarcane under organic versus conventional cultivation and prospection of plant growth promoting microorganisms

• Grant number: 17/09008-5
• Support Opportunities: Regular Research Grants
• Duration: August 01, 2017 - January 31, 2020
• Field of knowledge: Interdisciplinary Subjects
• Principal Investigator: Daniel Guariz Pinheiro
• Grantee: Daniel Guariz Pinheiro
• Host Institution: Faculdade de Ciências Agrárias e Veterinárias (FCAV). Universidade Estadual Paulista (UNESP). Campus de Jaboticabal. Jaboticabal , SP, Brazil
• Associated researchers: Eliana Gertrudes de Macedo Lemos ; Jairo Osvaldo Cazetta ; Lúcia Maria Carareto Alves ; Luciano Takeshi Kishi ; Marcos Antônio Soares ; Maria Inês Tiraboschi Ferro

Abstract

In recent years, industrial agriculture of sugarcane has been characterized by an intensification of agricultural inputs and expansion of monoculture. On the other hand, there is an increasing demand for healthier and more sustainable natural products. In this sense, organic farming has been considered. However, to supply the demand, this agroecosystem must also be productive, which requires the adoption of adequate management practices, and which must be improved with greater knowledge of the related biogeochemical processes. In this context, the microorganisms are the protagonists, acting especially in the soil and in association with the plants. In order to evaluate the microorganism diversity and the colonization structure in the tissues and the rhizosphere of plants under two modes of cultivation (organic x conventional), we propose the use of high-throughput DNA sequencing for the study of microorganisms associated with sugarcane. Amplicons (16S and ITS) will be sequenced from samples of the surface or inside the roots, shoots and leaves. The data will be analyzed by means of taxonomic identification and functional profiles prediction, required for evaluation of diversity and co-occurrence of the Operational Taxonomic Units found. The sequencing of rhizosphere metagenomes will also be performed and the data will be analyzed via assembly approach followed by functional and taxonomic annotation. In addition, we intend to prospect plant growth promoting microorganisms by means of the cultivation of microbial communities, followed by biochemical and experimental evaluations of innoculated model plant (Setaria viridis). Thus, we intend to expand the knowledge about the complex relationships among microorganisms, plant and environment and, with this, to contribute with the development of solutions to the problem of increasing production yield avoiding damage to ecosystems. This should be the way to achieve sustainability in agriculture. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

LOPES DE CARVALHO, LUCAS AMOROSO; TEHERAN-SIERRA, LUIS GUILLERMO; GONCALVES FUNNICELLI, MICHELLI INACIO; DA SILVA, RAFAEL CORREIA; ZANELI CAMPANARI, MARIA FERNANDA; CORREA DE SOUZA, RAFAEL SOARES; ARRUDA, PAULO; SOARES, MARCOS ANTONIO; PINHEIRO, DANIEL GUARIZ. Farming systems influence the compositional, structural, and functional characteristics of the sugarcane-associated microbiome. MICROBIOLOGICAL RESEARCH, v. 252, NOV 2021. (17/09008-5)

CORREA MONEDA, ANA PAULA; LOPES DE CARVALHO, LUCAS AMOROSO; TEHERAN-SIERRA, LUIS GUILLERMO; GONCALVES FUNNICELLI, MICHELLI INACIO; PINHEIRO, DANIEL GUARIZ. Sugarcane cultivation practices modulate rhizosphere microbial community composition and structure. SCIENTIFIC REPORTS, v. 12, n. 1, p. 19-pg., 2022-11-10. (17/09008-5)

TEHERAN-SIERRA, LUIS GUILLERMO; GONCALVES FUNNICELLI, MICHELLI INACIO; LOPES DE CARVALHO, LUCAS AMOROSO; TIRABOSCHI FERRO, MARIA INES; SOARES, MARCOS ANTONIO; PINHEIRO, DANIEL GUARIZ. Bacterial communities associated with sugarcane under different agricultural management exhibit a diversity of plant growth-promoting traits and evidence of synergistic effect. MICROBIOLOGICAL RESEARCH, v. 247, JUN 2021. (17/09008-5)