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Study of fungal clinical isolates from different anatomical sites and metagenomic of the lung microbiome of patients with cystic fibrosis

Grant number: 17/25300-8
Support type:Regular Research Grants
Duration: February 01, 2018 - July 31, 2020
Field of knowledge:Biological Sciences - Genetics - Molecular Genetics and Genetics of Microorganisms
Principal Investigator:Marcia Regina von Zeska Kress
Grantee:Marcia Regina von Zeska Kress
Home Institution: Faculdade de Ciências Farmacêuticas de Ribeirão Preto (FCFRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil
Assoc. researchers:Anderson Ferreira da Cunha ; Carolina Paulino da Costa Capizzani ; Érika Nascimento ; Mario Henrique Paziani ; Roberto Martinez
Associated scholarship(s):19/08383-2 - The study of clinical isolates fungi and the metagenomics of the lung microbiome of patients with fibrosis cystic, BP.TT

Abstract

Microorganisms and their balance in the human body are necessary to maintain the health. Once this equilibrium is broken, the excessive growth of pathogens occurs. Several factors lead to this imbalance, one of them is the mutation in the Transmembrane Conductivity Regulator gene of Cystic Fibrosis that causes the interruption of the normal transfer of chlorine and sodium ions through the cell membranes that lead to dehydration and consequently increase of the viscosity of cellular secretions, which may lead to failure of the mucociliary pulmonary clearance of patients with cystic fibrosis (CF). The study of microbiome has been facilitated by the Next Generation Sequencing technology capable of even sequencing genetic material from non-cultivable microorganisms. The present project aims to study by metagenomics, the microbiome (bacteria and fungi) of the lung of patients with cystic fibrosis before and after antibiotic therapy; knowing the fungi and bacteria as well as the inventory of the genes related to virulence, antimicrobial resistance and others. Additionally, clinical isolates of pathogenic filamentous fungi of Aspergillus and Fusarium genera from several anatomical sites, including sputum of CF patients will be studied. The molecular identification, sensitivity to antifungal and antimicrobial photodynamic therapy with phenotiazinium photosensitizers, study of the virulence factors and virulence in the experimental model Galleria mellonella will bring important information regarding these pathogenic microorganisms. The results obtained here will provide a critical view and assist the clinical decision-making regarding the best therapeutic approach. (AU)

Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
GRIZANTE BARIAO, PATRICIA HELENA; TONANI, LUDMILLA; COCIO, TIAGO ALEXANDRE; MARTINEZ, ROBERTO; NASCIMENTO, ERIKA; VON ZESKA KRESS, MARCIA REGINA. Molecular typing, in vitro susceptibility and virulence of Cryptococcus neoformans/Cryptococcus gattii species complex clinical isolates from south-eastern Brazil. MYCOSES, v. 63, n. 12, p. 1341-1351, DEC 2020. Web of Science Citations: 0.
DE ALMEIDA, OTAVIO GUILHERME GONCALVES; CAPIZZANI, CAROLINA PAULINO DA COSTA; TONANI, LUDMILLA; GRIZANTE BARIAO, PATRICIA HELENA; DA CUNHA, ANDERSON FERREIRA; DE MARTINIS, ELAINE CRISTINA PEREIRA; TORRES, LIDIA ALICE GOMES MONTEIRO MARIN; VON ZESKA KRESS, MARCIA REGINA. The Lung Microbiome of Three Young Brazilian Patients With Cystic Fibrosis Colonized by Fungi. FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY, v. 10, NOV 11 2020. Web of Science Citations: 0.
PAZIANI, MARIO HENRIQUE; CARVALHO, LUDMILLA TONANI; CARVALHO MELHEM, MARCIA DE SOUZA; GOTTARDO DE ALMEIDA, MARGARETE TERESA; NADALETTO BONIFACIO DA SILVA, MARIA EMILIA; MARTINEZ, ROBERTO; SANTOS, CLEDIR; VON ZESKA KRESS, MARCIA REGINA. First Comprehensive Report of Clinical Fusarium Strains Isolated in the State of Sao Paulo (Brazil) and Identified by MALDI-TOF MS and Molecular Biology. MICROORGANISMS, v. 8, n. 1 JAN 2020. Web of Science Citations: 0.

Please report errors in scientific publications list by writing to: cdi@fapesp.br.