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Production, purification and characterization of Rhizomucor sp. and evaluation of its application in mature cheese production

Grant number: 06/06573-9
Support type:Scholarships in Brazil - Doctorate
Effective date (Start): June 01, 2007
Effective date (End): September 30, 2010
Field of knowledge:Agronomical Sciences - Food Science and Technology - Food Science
Principal researcher:Roberto da Silva
Grantee:Carolina Merheb Dini
Home Institution: Instituto de Biociências, Letras e Ciências Exatas (IBILCE). Universidade Estadual Paulista (UNESP). Campus de São José do Rio Preto. São José do Rio Preto , SP, Brazil

Abstract

Proteases are one of the most important groups of industrial enzymes, with considerable application in the food industry, especially in cheesemaking. Microbial milk clotting proteases, also known as microbial rennins, are aspartic proteases that catalyze milk clotting, and thus substitute bovine chymosin. These enzymes also have proteolytic qualities that contribute to the cheese ripening process. In preliminary tests carried out in our laboratory, a Rhizomucor sp. that was able to produce a protease capable of hydrolyzing milk k-casein was isolated. Based on these facts, after production optimization, purification and characterization, the enzymes potential for application in the dairy industry will be investigated. (AU)

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
MERHEB-DINI, CAROLINA; CABRAL, HAMILTON; LEITE, RODRIGO S. R.; ZANPHORLIN, LETICIA M.; OKAMOTO, DEBORA N.; BONILLA RODRIGUEZ, GUSTAVO O.; JULIANO, LUIZ; ARANTES, ELIANE C.; GOMES, ELENI; DA SILVA, ROBERTO. Biochemical and Functional Characterization of a Metalloprotease from the Thermophilic Fungus Thermoascus aurantiacus. Journal of Agricultural and Food Chemistry, v. 57, n. 19, p. 9210-9217, . (06/06573-9)
MERHEB-DINI, CAROLINA; CHIUCHI GARCIA, GRAZIELE APARECIDA; BARRETTO PENNA, ANA LUCIA; GOMES, ELENI; DA SILVA, ROBERTO. Use of a new milk-clotting protease from Thermomucor indicae-seudaticae N31 as coagulant and changes during ripening of Prato cheese. Food Chemistry, v. 130, n. 4, p. 859-865, . (06/06573-9, 10/00566-6)

Please report errors in scientific publications list by writing to: cdi@fapesp.br.

Filed patent(s) as a result of this research project

APLICAÇÃO DE PROTEASE MICROBIANA NO PROCESSO DE FABRICAÇÃO DE QUEIJO PI1101014-2 - Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) ; Universidade Estadual Paulista (UNESP) . Roberto da Silva; Carolina Merheb Dini; Eleni Gomes - March 2011, 24