Studies of DNA damage by textile dyes and the protective capacity of flavonoids using electrochemical biosensor

• Grant number: 08/08990-1
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: March 01, 2009
• End date: February 28, 2013
• Field of knowledge: Physical Sciences and Mathematics - Chemistry - Analytical Chemistry
• Principal Investigator: Hideko Yamanaka
• Grantee: Carolina Venturini Uliana
• Host Institution: Instituto de Química (IQ). Universidade Estadual Paulista (UNESP). Campus de Araraquara. Araraquara , SP, Brazil

Abstract

Many pathophysiological conditions are related to the involvement of free radicals in the metabolism of living cells, for example, cellular aging, atherosclerosis, diabetes, various degenerative neurological diseases and cancer. These disorders are associated with a phenomenon called oxidative stress, which may be defined as an imbalance between species pro-oxidants and antioxidants in biological systems. External factors such as exposure to toxic substances, such as textile dyes, may also cause oxidative stress. Several studies have demonstrated the antioxidant effects of natural products in the prevention of damage caused by the oxidative process. Antioxidants have the property to capture free radicals and are, therefore, effective in combating the harmful effects of cellular oxidation process. Particularly, flavonoids, extracted from plants, present themselves as potential antioxidants. Therefore, this project proposes to investigate the interaction of azo dye with DNA immobilized on electrodes in order to obtain a greater understanding of these genotoxic substances, and the effect of antioxidant flavonoids present in plant extracts against the action of the textile dye in case damage to nucleotides.

Scientific publications

(7)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

ULIANA, CAROLINA V.; GARBELLINI, GUSTAVO S.; YAMANAKA, HIDEKO. Electrochemical reduction of disperse orange 1 textile dye at a boron-doped diamond electrode. Journal of Applied Electrochemistry, v. 42, n. 5, p. 297-304, MAY 2012. (09/08161-8, 08/08990-1, 08/10449-7)

ULIANA, CAROLINA VENTURINI; GARBELLINI, GUSTAVO STOPPA; YAMANAKA, HIDEKO. Electrochemical investigations on the capacity of flavonoids to protect DNA against damage caused by textile disperse dyes. SENSORS AND ACTUATORS B-CHEMICAL, v. 192, p. 188-195, MAR 2014. (09/08161-8, 08/08990-1, 08/10449-7)

ULIANA, CAROLINA VENTURINI; GARBELLINI, GUSTAVO STOPPA; YAMANAKA, HIDEKO. Evaluation of the interactions of DNA with the textile dyes Disperse Orange 1 and Disperse Red 1 and their electrolysis products using an electrochemical biosensor. SENSORS AND ACTUATORS B-CHEMICAL, v. 178, p. 627-635, MAR 1 2013. (09/08161-8, 08/08990-1, 08/10449-7)

GUSTAVO S. GARBELLINI; CAROLINA V. ULIANA; HIDEKO YAMANAKA. Interaction of organophosphorus pesticides with DNA nucleotides on a boron-doped diamond electrode. Journal of the Brazilian Chemical Society, v. 24, n. 12, p. 1942-1949, 2013-12-00. (08/08990-1, 09/08161-8)

ULIANA, CAROLINA V.; GARBELLINI, GUSTAVO S.; YAMANAKA, HIDEKO. Spectrophotometric evaluation of the behavior of disperse red 1 dye in aqueous media and its interaction with calf thymus ds-DNA. Journal of the Brazilian Chemical Society, v. 23, n. 8, p. 1469-1475, AUG 2012. (08/10449-7, 08/08990-1, 09/08161-8)

GARBELLINI, GUSTAVO S.; ULIANA, CAROLINA V.; YAMANAKA, HIDEKO. Detection of DNA nucleotides on pretreated boron doped diamond electrodes. Journal of the Brazilian Chemical Society, v. 22, n. 7, p. 1241-1245, 2011. (08/08990-1, 09/08161-8)

DA ROCHA, CAROLINA G.; ULIANA, V, CAROLINA; YAMANAKA, HIDEKO. On the Use of a TiO2 Sol-Gel for Horseradish Peroxidase Enzyme Immobilization for Development of an Amperometric Biosensor. Journal of the Brazilian Chemical Society, v. 32, n. 6, p. 1213-1221, JUN 2021. (08/08990-1, 14/50945-4)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

ULIANA, Carolina Venturini. Estudos da lesão ao DNA por corantes têxteis e da capacidade protetora de flavonóides empregando biossensor eletroquímico: Carolina Venturini Uliana. -. 2013. Doctoral Thesis - Universidade Estadual Paulista (Unesp). Instituto de Química. Araraquara Araraquara.