Possible cytoprotective effects of the dietary antioxidant coenzyme Q10 in a neuronal cell model

• Grant number: 09/11061-5
• Support Opportunities: Scholarships in Brazil - Master
• Start date: March 01, 2010
• End date: July 31, 2011
• Field of knowledge: Health Sciences - Nutrition - Nutrition Biochemistry
• Principal Investigator: Lusânia Maria Greggi Antunes
• Grantee: Carla da Silva Machado
• Host Institution: Faculdade de Ciências Farmacêuticas de Ribeirão Preto (FCFRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil

Abstract

In the last years, our research group has focused on the search line of investigation of different antioxidants found in the diet and their interactions with the chemotherapeutic cisplatin and doxorubicin, drugs widely used to treat various types of cancers. The aim has been to identify protective effects of antioxidants on the adverse effects induced in healthy cells by treatment with chemotherapy, and is important for this protective effect does not influence the activity of the drug on tumoral cells. In literature are described several strategies for treatment with the association of natural or synthetic compounds with benefits for the cancer patient. Recently, our laboratory was estabilished in the line of Nutrigenomics research, that advocates the use of tools of the molecular biology to investigate in greater detail the gene-diet interaction. The goal is to expand on molecular level, knowledge about the mechanism of action to the antioxidants. Thus, taking into account evidence from epidemiological studies that have shown the beneficial effects of diets rich in antioxidants against the development of neurodegenerative diseases, we believe the potential for inhibition of neurotoxicity of antioxidants in the diet could be extended to adverse effects that arise during chemotherapy. The relationship between antitumor chemotherapy with cisplatin and the development of sensory neuropathy due to damage induced in primary neurons has been confirmed in oncologic patients. The hypothesis of this project is that coenzyme Q10 can reduce the toxicity of the drug cisplatin in PC12 cells in culture. The neuroprotective potential of coenzyme Q10 will be evaluated by the inhibition of lipid peroxidation and by means of the parameters of analysis falls in single and double breats of DNA by the comet test and analysis of the "Cytoma" in binucleated cells. In addition, to identifying possible modulation in expression of the TP53 gene with the methodology of real-time PCR. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

MACHADO, CARLA DA SILVA; MENDONCA, LEONARDO MENEGHIN; VENANCIO, VINICIUS DE PAULA; PIRES BIANCHI, MARIA LOURDES; GREGGI ANTUNES, LUSANIA MARIA. Coenzyme Q10 protects Pc12 cells from cisplatin-induced DNA damage and neurotoxicity. NeuroToxicology, v. 36, p. 10-16, MAY 2013. (09/11061-5)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

MACHADO, Carla da Silva. Possible cytoprotective effects of the dietary antioxidant coenzyme Q10 in a neuronal cell model. 2011. Master's Dissertation - Universidade de São Paulo (USP). Faculdade de Ciências Farmacêuticas de Ribeirão Preto (PCARP/BC) Ribeirão Preto.