Study of E6 and E7 proteins from HPV16 and HPV6 and its effects on Toll like receptors signaling pathways

• Grant number: 11/14416-9
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: October 01, 2011
• End date: September 30, 2015
• Field of knowledge: Biological Sciences - Microbiology - Biology and Physiology of Microorganisms
• Principal Investigator: Luisa Lina Villa
• Grantee: Mirian Galliote Morale
• Host Institution: Instituto do Câncer do Estado de São Paulo Octavio Frias de Oliveira (ICESP). Coordenadoria de Serviços de Saúde (CSS). Secretaria da Saúde (São Paulo - Estado). São Paulo , SP, Brazil
• Associated research grant: 08/03232-1 - HPV and tumor microenvironment, AP.TEM

Abstract

High risk or oncogenic Human Papillomavirus (HPV) are responsible for the development of cervical cancer. In 2008 there were 529.800 new cases and 275.100 registered deaths of cervical cancer worldwide.Integration of the viral genome is an essential step to the development of cancer, resulting in loss or disruption of E2 gene, causing the continuous expression of E6 and E7.Besides several other interactions, both E6 and E7 are involved in deregulating the innate immune system interfering with the expression of Toll like receptors (TLR), which is an important step in cancer progression.Numerous studies indicate that TLR signaling contributes to tumor growth in different organs and their activation in cancer cells with subsequent production of cytokines and chemokines promote angiogenesis, cell survival, chemo resistance and thus tumor progression. However little is known about how HPV affects TLR signaling pathways during infection and cervical cancer progression.This project aims to study the effects of HPV infection on Toll like receptors (TLR) signaling pathways in human primary keratinocytes and cervical lesions.

Scientific publications

(4)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

CABECA, TATIANE KAREN; ABREU, ALICE DE MELLO; ANDRETTE, RAFAEL; LINO, VANESCA DE SOUZA; MORALE, MIRIAN GALLIOTE; AGUAYO, FRANCISCO; TERMINI, LARA; VILLA, LUISA LINA; LEPIQUE, ANA PAULA; BOCCARDO, ENRIQUE. HPV-Mediated Resistance to TNF and TRAIL Is Characterized by Global Alterations in Apoptosis Regulatory Factors, Dysregulation of Death Receptors, and Induction of ROS/RNS. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, v. 20, n. 1, JAN 1 2019. (11/14416-9, 08/03232-1, 10/20002-0, 08/57889-1)

MORALE, MIRIAN GALLIOTE; ABJAUDE, WALASON DA SILVA; SILVA, ALINE MONTENEGRO; VILLA, LUISA LINA; BOCCARDO, ENRIQUE. HPV-transformed cells exhibit altered HMGB1-TLR4/MyD88-SARM1 signaling axis. SCIENTIFIC REPORTS, v. 8, FEB 22 2018. (14/21361-4, 10/20002-0, 11/14416-9, 08/57889-1, 08/03232-1)

PRATI, BRUNA; ABJAUDE, WALASON DA SILVA; TERMINI, LARA; MORALE, MIRIAN; HERBSTER, SUELLEN; LONGATTO-FILHO, ADHEMAR; LIMA NUNES, RAFAELLA ALMEIDA; CORDOBA CAMACHO, LIZETH CAROLINA; RABELO-SANTOS, SILVIA HELENA; ZEFERINO, LUIZ CARLOS; et al. Three Prime Repair Exonuclease 1 (TREX1) expression correlates with cervical cancer cells growth in vitro and disease progression in vivo. SCIENTIFIC REPORTS, v. 9, JAN 23 2019. (08/57889-1, 10/20002-0, 13/27006-9, 12/16512-8, 11/14416-9)

BUGNON VALDANO, M.; CAVATORTA, A. L.; MORALE, M. G.; MARZIALI, F.; DE SOUZA LINO, V.; STEENBERGEN, R. D. M.; BOCCARDO, E.; GARDIOL, D.. Disc large 1 expression is altered by human papillomavirus E6/E7 proteins in organotypic cultures of human keratinocytes. JOURNAL OF GENERAL VIROLOGY, v. 97, p. 10-pg., 2016-02-01. (11/14416-9, 12/51017-8)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

MORALE, Mirian Galliote. Effects of HPV infection on TLR signaling pathway. 2016. Doctoral Thesis - Universidade de São Paulo (USP). Conjunto das Químicas (IQ e FCF) (CQ/DBDCQ) São Paulo.