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Influence of diferents genotypes in the pattern of selenoproteins expression in response to selenium supplementation

Grant number: 11/17720-0
Support type:Scholarships in Brazil - Doctorate
Effective date (Start): January 01, 2012
Effective date (End): April 30, 2016
Field of knowledge:Health Sciences - Nutrition
Principal Investigator:Silvia Maria Franciscato Cozzolino
Grantee:Janaina Lombello Santos Donadio
Home Institution: Faculdade de Ciências Farmacêuticas (FCF). Universidade de São Paulo (USP). São Paulo , SP, Brazil
Associated scholarship(s):15/10146-8 - Influence of genetic polymorphisms in SEPP1 gene on SePP and GPx3 concentrations after brazil nut supplementation, BE.EP.DR   13/03224-7 - Nutritional and genetic regulation of selenoprotein gene expression, BE.EP.DR

Abstract

BACKGROUND: Selenium (Se) is an essential micronutrient for human health, exerting their functions in the form of the amino acid selenocysteine, the 21st amino acid. Because of their importance in biological systems, the intake of Se have been monitored in several countries, and millions of people around the world can have mineral deficiency. This possible deficiency that probably affects millions of people, can contribute to increased risk of chronic diseases, those that most affect the world population. Several studies have shown evidence for the hypothesis that Se is a key factor for proper functioning immune system. The effect of Se supplementation has been evaluated in studies conducted in animal and humans models. Within this context, where different responses were observed in supplementation studies, the issue of genetic variability becomes increasingly evident, and the genetic profile of the participants can affect the response to supplementation. Currently, several polymorphisms in selenoproteins were shown to have functional consequences. OBJECTIVE: To evaluate the influence of genotypes in the pattern of expression of selenoproteins in response to supplementation with the Brazil nut. METHODOLOGY: The study will be approximately 100 individuals of both genders, aged 20 to 50 years. The population will be recruited within the University of São Paulo being the inclusion criteria: 1) not take vitamin and mineral supplements, 2) not taking anti-inflammatory, 3) not be an elite athlete, 4) not to consume alcohol chronically, 5) does not present liver diseases, cardiovascular diseases, diabetes and cancer and 6) do not take the Brazil nuts daily. Initially it will be performed for assessment of nutritional status in determining if the concentrations of plasma and erythrocyte, which will be reassessed after supplementation for 6 weeks with brown. From this time the volunteers will be monitored every two weeks add, however s 6 weeks to evaluate the washout period.The polymorphisms chosen for study were based on published data regarding potential functional consequences of SNPs as well as its relationship with increased disease risk. The gene expression profile will be evaluated by RT-PCR using Taqman-type assays, before and after supplementation (TO and T1). The genes chosen to investigate the difference in expression in response to supplementation with selenium, are GPx1, GPx3, GPx4, Seppi. Concentrations of total plasma cholesterol, LDL cholesterol, HDL-cholesterol, VLDL-cholesterol and triglycerides will be performed by commercial enzymatic methods (Labtest). If the concentrations of plasma and erythrocytes will be quantified by atomic absorption spectrometry coupled with hydride generation quartz cell (HGQTAAS). The determination of SOD and GPx activity in erythrocytes will be held with the aid of commercial kits (Ransel 505 - Randox Laboratories, Crumlin / UK) adapted for use in automatic biochemical analyzer (240 Labmax, Labtest).Dietary intake of participants will be assessed through five 24-hour recall, (one for each collection time) The questionnaires will be evaluated in Avanutri software, Statistical analysis will be performed in the SPSS and Statistica.

Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
DONADIO, JANAINA L. S.; ROGERO, MARCELO M.; GUERRA-SHINOHARA, ELVIRA M.; BARBOSA, JR., FERNANDO; DESMARCHELIER, CHARLES; BOREL, PATRICK; SNEDDON, ALAN A.; HESKETH, JOHN E.; COZZOLINO, SILVIA M. F. Genetic variants in selenoprotein genes modulate biomarkers of selenium status in response to Brazil nut supplementation (the SU.BRA.NUT study). Clinical Nutrition, v. 38, n. 2, p. 539-548, APR 2019. Web of Science Citations: 0.
DONADIO, JANAINA L. S.; ROGERO, MARCELO M.; GUERRA-SHINOHARA, ELVIRA M.; DESMARCHELIER, CHARLES; BOREL, PATRICK; COZZOLINO, SILVIA M. F. SEPP1 polymorphisms modulate serum glucose and lipid response to Brazil nut supplementation. EUROPEAN JOURNAL OF NUTRITION, v. 57, n. 5, p. 1873-1882, AUG 2018. Web of Science Citations: 2.
DONADIO, JANAINA L. S.; ROGERO, MARCELO M.; COCKELL, SIMON; HESKETH, JOHN; COZZOLINO, SILVIA M. F. Influence of Genetic Variations in Selenoprotein Genes on the Pattern of Gene Expression after Supplementation with Brazil Nuts. NUTRIENTS, v. 9, n. 7 JUL 2017. Web of Science Citations: 4.

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