Systemic metronidazole and amoxicillin as adjuncts to scaling and root planing in the treatment of chronic periodontitis in type 2 diabetic subjects: clinical, glycemic and microbiological evaluations

• Grant number: 11/14872-4
• Support Opportunities: Scholarships in Brazil - Master
• Start date: March 01, 2012
• End date: February 28, 2014
• Field of knowledge: Health Sciences - Dentistry - Periodontology
• Principal Investigator: Poliana Mendes Duarte
• Grantee: Tamires Szeremeske de Miranda
• Host Institution: Universidade de Guarulhos (UNG). Campus Guarulhos-Centro. Guarulhos , SP, Brazil

Abstract

The aim of this double-blinded, prospective, randomized, placebo-controlled clinical trial will be to evaluate, at 3 and 6 months, the clinical, glycemic and microbiological effects of systemic metronidazole and amoxicillin as adjunctive to scaling and root planing (SRP) in uncontrolled type 2 diabetic subjects with chronic periodontitis. Forty subjects with chronic periodontitis and type 2 diabetes will be divided into one of the following therapeutic groups: Control (n=20) SRP + placebo; Test (n=20): RAR + systemic metronidazole (400mg) + amoxicillin (500mg), 3x/day for 10 days. The following clinical parameters will be evaluated: probing depth (PD), clinical attachment level (CAL), visible plaque accumulation, bleeding on probing, marginal bleeding and suppuration. Six subgingival biofilm samples will be collected per subjects (3 samples PD < 5 mm e 3 samples PD e 5 mm) microbiological evaluation by quantitative PCR of the bacterial species: Prevotella intermedia, Porphyromonas gingivalis, Tanerella forsythia, Eubacterium nodatum and Parvimona micra. Clinical and microbiological parameters and the levels of glycated hemoglobin and fasting plasma glucose will be assessed before and at 3 and 6 months post-therapies. Intra-group comparisons (before and after treatment) will be performed by Friedman test or repeated measured ANOVA, according to data normality. Inter-group comparisons (control/test) within each time-point will be performed by Mann-Whitney test and t-test. The level of significance will be set at 5%.

Scientific publications

(8)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

MIRANDA, TAMIRES SZEREMESKE; FERES, MAGDA; PEREZ-CHAPARRO, PAULA JULIANA; FAVERI, MARCELO; FIGUEIREDO, LUCIENE CRISTINA; TAMASHIRO, NEILA SUMIE; BASTOS, MARTA FERREIRA; DUARTE, POLIANA MENDES. Metronidazole and amoxicillin as adjuncts to scaling and root planing for the treatment of type 2 diabetic subjects with periodontitis: 1-year outcomes of a randomized placebo-controlled clinical trial. Journal of Clinical Periodontology, v. 41, n. 9, p. 890-899, SEP 2014. (11/14872-4, 13/01072-5)

TAMASHIRO, N. S.; DUARTE, P. M.; MIRANDA, T. S.; MACIEL, S. S.; FIGUEIREDO, L. C.; FAVERI, M.; FERES, M.. Amoxicillin Plus Metronidazole Therapy for Patients with Periodontitis and Type 2 Diabetes: A 2-year Randomized Controlled Trial. JOURNAL OF DENTAL RESEARCH, v. 95, n. 7, p. 829-836, JUL 2016. (11/14872-4, 13/01072-5)

MIRANDA, TAMIRES SZEREMESKE; FERES, MAGDA; RETAMAL-VALDES, BELEN; PEREZ-CHAPARRO, PAULA JULIANA; MACIEL, SUELLEN SILVA; DUARTE, POLIANA MENDES. Influence of glycemic control on the levels of subgingival periodontal pathogens in patients with generalized chronic periodontitis and type 2 diabetes. Journal of Applied Oral Science, v. 25, n. 1, p. 82-89, JAN-FEB 2017. (11/14872-4, 13/01072-5)

RETAMAL-VALDES, BELEN; TAVARES, ANA PAULA LARANJEIRA; MONIQUE, SARAH; PEREIRA DA SILVA, HELIO DOYLE; MESTNIK, MARIA JOSEFA; DUARTE, POLIANA MENDES; MIRANDA, TAMIRES S.; BORGES, IVAN; SOARES, GEISLA MARY SILVA; FAVERI, MARCELO; et al. Adverse events of metronidazole and amoxicillin: Retrospective analysis of a large data set of five randomized clinical trials. JOURNAL OF CLINICAL PERIODONTOLOGY, v. 49, n. 11, p. 12-pg., 2022-08-05. (09/17677-8, 11/14872-4, 16/10958-5, 13/01072-5, 07/56413-0)

CRUZ, DANIELE FERREIRA DA; DUARTE, POLIANA MENDES; DE FIGUEIREDO, LUCIENE CRISTINA; DA SILVA, HELIO DOYLE PEREIRA; RETAMAL-VALDES, BELEN; FERES, MAGDA; MIRANDA, TAMIRES SZEREMESKE. Metronidazole and amoxicillin for patients with periodontitis and diabetes mellitus: 5 years secondary analysis of a randomized controlled trial. Journal of Periodontology, v. 92, n. 4, p. 479-487, APR 2021. (11/14872-4)

DUARTE, POLIANA MENDES; FERES, MAGDA; SMEILI YASSINE, LINA LAMEH; SILVA SOARES, GEISLA MARY; MIRANDA, TAMIRES SZEREMESKE; FAVERI, MARCELO; RETAMAL-VALDES, BELEN; FIGUEIREDO, LUCIENE CRISTINA. Clinical and microbiological effects of scaling and root planing, metronidazole and amoxicillin in the treatment of diabetic and non-diabetic subjects with periodontitis: A cohort study. JOURNAL OF CLINICAL PERIODONTOLOGY, v. 45, n. 11, p. 1326-1335, NOV 2018. (11/14872-4, 13/01072-5)

DUARTE, POLIANA M.; FELIX, EDCARLOS; SANTOS, VANESSA R.; FIGUEIREDO, LUCIENE C.; DA SILVA, HELIO D. P.; MENDES, JULIANA A. V.; FERES, MAGDA; MIRANDA, TAMIRES S.. Patients with type 2 diabetes and severe periodontitis harbor a less pathogenic subgingival biofilm than normoglycemic individuals with severe periodontitis. Journal of Periodontology, v. N/A, p. 10-pg., 2023-05-24. (13/10354-4, 11/14872-4, 08/11419-4, 08/09687-0, 13/23743-9, 13/01072-5, 09/17677-8)

MIRANDA, TAMIRES SZEREMESKE; FERES, MAGDA; PEREZ-CHAPARRO, PAULA JULIANA; FAVERI, MARCELO; FIGUEIREDO, LUCIENE CRISTINA; TAMASHIRO, NEILA SUMIE; BASTOS, MARTA FERREIRA; DUARTE, POLIANA MENDES. Metronidazole and amoxicillin as adjuncts to scaling and root planing for the treatment of type 2 diabetic subjects with periodontitis: 1-year outcomes of a randomized placebo-controlled clinical trial. JOURNAL OF CLINICAL PERIODONTOLOGY, v. 41, n. 9, p. 10-pg., 2014-09-01. (13/01072-5, 11/14872-4)