Infiltrant application on sound enamel and with initial erosion lesion, subjjected to in vitro erosive challenge

• Grant number: 11/16208-4
• Support Opportunities: Scholarships in Brazil - Master
• Start date: March 01, 2012
• End date: May 31, 2013
• Field of knowledge: Health Sciences - Dentistry - Pediatric Dentistry
• Principal Investigator: Daniela Rios Honório
• Grantee: Gabriela Cristina de Oliveira
• Host Institution: Faculdade de Odontologia de Bauru (FOB). Universidade de São Paulo (USP). Bauru , SP, Brazil

Abstract

The infiltrant is able to penetrate into sound or demineralised dental tissues, increasing tooth resistance against caries lesions progression, similar effect could be expected to dental erosion. However, it is unclear to what extent the surface conditioning for the infiltrant penetration, might be able to remove the softened surface of the initial erosion lesion. Thus, the aim of the present study is to evaluate the effect of infiltrant application on sound enamel and with initial erosion lesion, on the prevention of subsequent enamel loss. In addition, the influence of the acid etching on initial erosion lesion will be studied. The study will be divided in two in vitro protocols,totalizing 15 groups (n = 15). On G1 to G5 (protocol 1) and GLE 1 to GLE 5 (protocol 2), the studied materials willl be applied in accordance with the manufacturer's, preceded by etching conditioning for adhesion.On protocol 2, no acid etching prior to material application will be conducted: G1 without treatment - negative control, G2 resin sealant application - Helioseal Clear, G3 Light- and dual-curing, self-etching adhesive application - Adhese, G4 light-curing bonding agent application - Heliobond, G5 application infiltrant - Icon. After bovine blocks preparation,superficial microhardness will be performed for selection and random division of blocks into groups on protocol 1. On protocol 2 initial erosion lesion (softened surface)will be developed. Before treatments of each group, the blocks will have two thirds of its surfaces protected with nail varnish. After the treatments erosive cycling will start, consisting of four daily immersions in acidic solution for 2 min followed by immersion in artificial saliva for 2 h, for 5 days. After treatment (application of the resinous material)and after the 5th day of erosive cycling, enamel surface will be evaluated by profilometry. The results will be subjected to appropriate statistical analysis (p <0.05). (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DE OLIVEIRA, GABRIELA CRISTINA; GENOVEZ TEREZA, GUIDA PAOLA; BOTEON, ANA PAULA; FERRAIRO, BRUNNA MOTA; PINTO GONCALVES, PRISCILLA SANTANA; DA SILVA, THIAGO CRUVINEL; HONORIO, HEITOR MARQUES; RIOS, DANIELA. Susceptibility of bovine dental enamel with initial erosion lesion to new erosive challenges. PLoS One, v. 12, n. 8, AUG 17 2017. (11/12395-4, 11/11814-3, 11/16208-4)

OLIVEIRA, G. C.; BOTEON, A. P.; IONTA, F. Q.; MORETTO, M. J.; HONORIO, H. M.; WANG, L.; RIOS, D.. In Vitro Effects of Resin Infiltration on Enamel Erosion Inhibition. Operative Dentistry, v. 40, n. 5, p. 492-502, SEP-OCT 2015. (11/12395-4, 11/16208-4)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

OLIVEIRA, Gabriela Cristina de. Uso de infiltrante sobre esmalte hígido e com lesão inicial de erosão submetido a desafio erosivo in vitro. 2013. Master's Dissertation - Universidade de São Paulo (USP). Faculdade de Odontologia de Bauru (FOB/SDB) Bauru.