MICROENCAPSULATION OF SQUALENE FROM DOGFISH LIVER OIL THROUGH PRECIPITATION WITH SUPERCRITICAL CO2 USING THE RESS TECHNIQUE

• Grant number: 12/00467-3
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: June 01, 2012
• End date: February 29, 2016
• Field of knowledge: Agronomical Sciences - Food Science and Technology - Food Engineering
• Principal Investigator: Alessandra Lopes de Oliveira
• Grantee: Débora Nascimento e Santos
• Host Institution: Faculdade de Zootecnia e Engenharia de Alimentos (FZEA). Universidade de São Paulo (USP). Pirassununga , SP, Brazil

Abstract

The cação liver oil has a high nutritional value and is rich in squalene, a hydrocarbon compound which researches show an anticancer activity, antioxidant, radio protective agent, enhances of the immune system, among others. One way to prolong the beneficial effects of this class of substances is through microencapsulation technology, which is constantly advancing. Microencapsulation with supercritical fluids has been utilized to advantage of soft temperatures of the process and reducing the use of organic solvent. The objectives of this project are to obtain the cação liver oil rich in squalene by supercritical fluid extraction technique and using the RESS (Rapid Expansion of Supercritical Solution) to beginning the microparticles of the cação liver oil. The viscera collected will be homogenized and lyophilized. The supercritical extraction conditions will be defined according DCCR and among the oils obtained, which has a higher content of squalene is used for the RESS microencapsulation technique in different operating conditions and with four different ratios between the encapsulating agent (maltrodextrine) and the cacao liver oil. After obtaining the microcapsules, they will be evaluated for encapsulation efficiency, morphology and size, oxidative stability of oil, squalene's ability to release, among other parameters. It is hoped to obtain oils with higher levels of squalene obtained by the conventional methods and that their respective microcapsules have good properties when evaluated. The results of this project will contribute to the expansion of knowledge in the area, since there are few studies on the microencapsulation of oils using a new technology such as RESS.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

SANTOS, D. N.; SILVA, F. S.; VERDE, A. B.; BITTENCOURT, G. M.; DE OLIVEIRA, A. L.. Determination of functional compounds in blue shark (Prionace glauca) liver oil obtained by green technology. GRASAS Y ACEITES, v. 71, n. 2, APR-JUN 2020. (12/06981-0, 13/18347-7, 12/00467-3)

SANTOS, DEBORA NASCIMENTO E; AREDO, VICTOR; BAZITO, REINALDO CAMINO; DE OLIVEIRA, ALESSANDRA LOPES. Water free incorporation of shark liver oil into starch microparticles by supercriticalCO(2)impregnation at low temperature. JOURNAL OF FOOD PROCESS ENGINEERING, v. 43, n. 11, NOV 2020. (12/06981-0, 12/00467-3)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

SANTOS, Débora Nascimento e. Microencapsulation of squalene from blue shark liver oil by supercritical CO2 precipitation. 2016. Doctoral Thesis - Universidade de São Paulo (USP). Faculdade de Zootecnica e Engenharia de Alimentos (FZE/BT) Pirassununga.