Virulence evaluation and Differential gene expression in vivo of Salmonella enterica mutants for nucleoid associated proteins

• Grant number: 12/05382-6
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: June 01, 2012
• End date: February 29, 2016
• Field of knowledge: Biological Sciences - Microbiology - Applied Microbiology
• Principal Investigator: Marcelo Brocchi
• Grantee: Guilherme Paier Milanez
• Host Institution: Instituto de Biologia (IB). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil

Abstract

Salmonella enterica is a rod-shaped, gram-negative, facultative anaerobic, Enterobacteriaceae family member, responsible for great economic losses and public health problems. It causes disease to humans and animals, ranging from gastroenteritis to systemic infections. Approximately 20 years ago, the Enteritidis serovar became the most present in cases of salmonellosis in Brazil, Europe and the United States, being eggs and other products derived from chicken the main source of infection. One way to control those infections caused by S. enterica is using vaccines, which has already been used in several countries, despite the need of improvement of these. Recent data from our group indicate that null mutants of S. enterica Typhimurium for NAP (Nucleoid-associated proteins) genes, are attenuated for virulence and capable of inducing protection in a murine model of infection. The patents about the development of these strains have been submitted to the INPI. Based on S. enterica Typhimurium recent data from our group, this project proposes the construction of mutants of S. enterica Enteritidis for genes hupA, hupB and the double-mutant hupAhupB through »-Red homologous recombination system, and characterization of those mutants in attenuation of virulence and the immune response generated in a murine model of infection. These are histone-like genes that acts in the global regulation of gene expression, so the differences in gene expression profile of S. Enteritidis new mutants will be analised, as well as the previously constructed in S. Typhimurium, using RNA-seq technique, and the data will be validated by RT-PCR. We hope to obtain important data showing genetic differences between strains, and reflecting the difference between their mechanisms of pathogenicity.

Scientific publications

(6)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

MILANEZ, GUILHERME P.; WERLE, CATIERINE H.; AMORIM, MARIENE R.; RIBEIRO, RAFAEL A.; TIBO, LUIZ H. S.; ROQUE-BARREIRA, MARIA CRISTINA; OLIVEIRA, ALINE F.; BROCCHI, MARCELO. HU-Lacking Mutants of Salmonella enterica Enteritidis Are Highly Attenuated and Can Induce Protection in Murine Model of Infection. FRONTIERS IN MICROBIOLOGY, v. 9, AUG 22 2018. (13/04088-0, 12/05382-6, 14/13412-8, 14/05359-0)

TOMIYAMA, M. P. O.; WERLE, C. H.; MILANEZ, G. P.; NOBREGA, D. B.; PEREIRA, J. P.; CALARGA, A. P.; FLORES, F.; BROCCHI, M.. Salmonella enterica Typhimurium fljBA operon stability: implications regarding the origin of Salmonella enterica I 4,[5], 12:i:-. Genetics and Molecular Research, v. 14, n. 4, p. 19057-19065, 2015. (09/15956-7, 14/11280-7, 13/11880-1, 12/10608-3, 12/05382-6)

LEITE, BRUNA; WERLE, CATIERINE HIRSCH; DO CARMO, CAMILA PINHEIRO; NOBREGA, DIEGO BORIN; MILANEZ, GUILHERME PAIER; CULLER, HEBERT FABRICIO; SIRCILI, MARCELO PALMA; ALVAREZ-MARTINEZ, CRISTINA E.; BROCCHI, MARCELO. Integration host factor is important for biofilm formation by Salmonella enterica Enteritidis. PATHOGENS AND DISEASE, v. 75, n. 6, AUG 2017. (12/10608-3, 12/25426-8, 12/05382-6)

MILANEZ, GUILHERME PAIER; NASCIMENTO, LEANDRO COSTA; TIRABASSI, ADRIANE HOLTZ; ZUANAZE, MARCELO; RODRIGUES, DALIA PRAZERES; GUIMARAES PEREIRA, GONCALO AMARANTE; BROCCHI, MARCELO. Whole-Genome Sequence of Salmonella enterica Serovar Enteritidis Phage Type 4, Isolated from a Brazilian Poultry Farm. MICROBIOLOGY RESOURCE ANNOUNCEMENTS, v. 4, n. 3, MAY-JUN 2016. (14/13412-8, 12/05382-6)

WERLE, CATIERINE HIRSCH; DAMIANI, IGOR; MILANEZ, GUILHERME PAIER; FARIAS, ALESSANDRO S.; CINTRA GOMES MARCONDES, MARIA CRISTINA; CULLER, HEBERT FABRICIO; SIRCILI, MARCELO PALMA; LEITE, BRUNA; BROCCHI, MARCELO. Antimelanoma effect of Salmonella Typhimurium integration host factor mutant in murine model. FUTURE ONCOLOGY, v. 12, n. 20, p. 2367-2378, OCT 2016. (12/25426-8, 12/05382-6, 14/13412-8)

MILANEZ, GUILHERME PAIER; NASCIMENTO, LEANDRO COSTA; TIRABASSI, ADRIANE HOLTZ; ZUANAZE, MARCELO; RODRIGUES, DALIA PRAZERES; GUIMARAES PEREIRA, GONCALO AMARANTE; BROCCHI, MARCELO. Whole-Genome Sequence of Salmonella enterica Serovar Enteritidis Phage Type 4, Isolated from a Brazilian Poultry Farm. GENOME ANNOUNCEMENTS, v. 4, n. 3, p. 2-pg., 2016-05-01. (14/13412-8, 12/05382-6)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

MILANEZ, Guilherme Paier. Virulence evaluation and potential use as vaccine of Salmonella enterica mutants for nucleoid associated proteins. 2016. Doctoral Thesis - Universidade Estadual de Campinas (UNICAMP). Instituto de Biologia Campinas, SP.