Influence of lycopene on oxidative stress markers in adipose tissue of obese rats

• Grant number: 11/22786-0
• Support Opportunities: Scholarships abroad - Research Internship - Post-doctor
• Start date: May 01, 2012
• End date: April 30, 2013
• Field of knowledge: Health Sciences - Nutrition - Nutrition Biochemistry
• Principal Investigator: Ana Lucia do Anjos Ferreira
• Grantee: Renata de Azevedo Melo Luvizotto Nascimento
• Supervisor: Kyung-Jin Yeum
• Host Institution: Faculdade de Medicina (FMB). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil
• Institution abroad: Tufts University, United States
• Associated to the scholarship: 10/19746-4 - Effect of lycopene supplementation in experimental model of diet-induced obesity, BP.PD

Abstract

In obesity, the increased oxidative stress levels can be a cause of adipose tissue functional alterations. Given the importance of oxidative stress in pathogenesis of adipose tissue, therapeutic strategy using lycopene, a potent antioxidant, may be relevant in an attempt to reduce the ROS overproduction and minimize the consequences of dysfunctional adipocyte. The aim of this study is focus efforts to assess the oxidative stress markers, as well as the influence of lycopene chronic administration on oxidative stress markers in adipose tissue of animals subjected to hypercaloric diet. Male Wistar rats (n=28) will be randomly separated for receiving control diet (C) or hypercaloric diet (H) for six weeks. After this period, all animals will be again randomized into four groups: C (n=7), C supplemented with Lycopene (C+L, n=7), H (n=7) or H supplemented with lycopene (H+L, n=7). The lycopene used will be oil-resin mixed with corn oil (10mg/Kg body weight/day); untreated groups (c and H) will receive corn oil at an equivalent volume (~ 0.5 mL/kg/day), both via gavage, for six weeks. Oxidative stress markers will be analyzed indirectly by the end products of lipid peroxidation presence, malondialdehyde and 4-hydroxynonenal; by DNA damage; by proteins nitration and carbonylation; and total antioxidant performance. Data will be expressed by descriptive measures of position and variability. The comparison between groups will be performed by ANOVA complemented by Tukey test. The adopted significance level will be 5%. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

MELO LUVIZOTTO, RENATA DE AZEVEDO; NASCIMENTO, ANDRE F.; IMAIZUMI, ERIKA; PIERINE, DAMIANA T.; CONDE, SANDRO J.; CORREA, CAMILA R.; YEUM, KYUNG-JIN; FERREIRA, ANA LUCIA A.. Lycopene supplementation modulates plasma concentrations and epididymal adipose tissue mRNA of leptin, resistin and IL-6 in diet-induced obese rats. BRITISH JOURNAL OF NUTRITION, v. 110, n. 10, p. 1803-1809, NOV 28 2013. (11/22786-0, 10/19746-4, 11/19847-8, 10/06100-9)

LUVIZOTTO, R. A. M.; NASCIMENTO, A. F.; MIRANDA, N. C. M.; WANG, X-D; FERREIRA, A. L. A.. Lycopene-rich tomato oleoresin modulates plasma adiponectin concentration and mRNA levels of adiponectin, SIRT1, and FoxO1 in adipose tissue of obese rats. HUMAN & EXPERIMENTAL TOXICOLOGY, v. 34, n. 6, p. 612-619, JUN 2015. (11/22786-0, 11/19934-8, 10/19746-4, 10/06100-9)