Study of P2X7 receptor in the myenteric sinaptic transmission after ischemia and reperfusion of the ileum

• Grant number: 12/06434-0
• Support Opportunities: Scholarships abroad - Research Internship - Master's degree
• Start date: September 01, 2012
• End date: November 30, 2012
• Field of knowledge: Biological Sciences - Morphology - Anatomy
• Principal Investigator: Patricia Castelucci
• Grantee: Cristina Eusébio Mendes
• Supervisor: Paulo Jorge Silva Correia Sa
• Host Institution: Instituto de Ciências Biomédicas (ICB). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• Institution abroad: Universidade do Porto (UP), Portugal
• Associated to the scholarship: 10/12918-4 - Study of enteric glial cells immunoreactive for P2X2 and P2X7 receptors in the enteric nervous system of the ileum of rats subjected to ischemia and reperfusion, BP.MS

Abstract

The enteric nervous system is composed of the myenteric and submucosal plexuses, which control motility, the transport of fluids from the mucosa, and local blood flow. These plexuses are formed by neurons and glial cells. Intestinal ischemia/reperfusion (I/R-i) injury is a common, and devastating problem in hospitalized patients, and is associated with high morbidity and mortality in both surgical and trauma patients. Studies have suggested that the small intestine is one of the organs most susceptible to I/R-i, such as that encountered during hemorrhagic shock or small bowel transplantation (RÜHL, 2005; PAULINO et al., 2011). This work aims contribute in original way using functional assays, combined with the results obtained with immunohistochemistry, to understand the effects of different periods of ischemia with reperfusion on the neuro-glial communication in the enteric nervous system mediated by P2X7 receptors. The results of this collaboration will help clarify the role of these receptors in the regulation of intestinal motility after events of ischemia/reperfusion and whether the ATP through its action on P2X7 receptor, could provide a new therapeutic for the treatment of gastrointestinal disorders. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

MENDES, CRISTINA EUSEBIO; PALOMBIT, KELLY; VIEIRA, CATIA; SILVA, ISABEL; CORREIA-DE-SA, PAULO; CASTELUCCI, PATRICIA. The Effect of Ischemia and Reperfusion on Enteric Glial Cells and Contractile Activity in the Ileum. Digestive Diseases and Sciences, v. 60, n. 9, p. 2677-2689, SEP 2015. (12/06434-0, 08/08728-5, 12/00259-1, 08/05314-5)