Platelet gel in articular cartilage defects: in vitro and in vivo study in sheep model

• Grant number: 12/04516-9
• Support type: Scholarships in Brazil - Scientific Initiation
• Effective date (Start): September 01, 2012
• Effective date (End): August 31, 2013
• Field of knowledge: Agronomical Sciences - Veterinary Medicine
• Principal Investigator: Ana Liz Garcia Alves
• Grantee: Caio Nunes de Barros
• Home Institution: Faculdade de Medicina Veterinária e Zootecnia (FMVZ). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil

Abstract

The platelet gel has been used as a sealant to control small bleedings and to induce the adherence of tissues. This kind of gel is mainly used helping in situations where the tissue repair capacity is limited, which is the case of the chondral injuries. This biomaterial is indicated in the construction of scaffolds, required in the maintenance of several cell types, due to its ability to stimulate the migration, differentiation and cell proliferation. The platelet gel is also rich in growth factors and is widely used on the treatment of articular cartilage defects. However, despite of knowledge about the advantages of this biomaterial and the domain of techniques of using it, the durability of platelet gel at the site of chondral injury is still discussed. Knowing the importance of this fact for the quality and efficiency of treatments that use platelet gel, this study aims to examine the durability and efficacy of treatments that use platelet gel through in vitro and in vivo studies. Thus, the platelet gel previously prepared from platelet-rich plasma obtained from healthy sheep, will kept in culture plates (in vivo study) in a first step. In a second step, the gel will be implanted singly, by arthrotomy, on chondral defects induced in the articular surface of the lateral femoral trochlea of eight animals (in vivo study). In the in vitro study, will be measured the gel durability through the form maintenance and the consistence on the culture plates. The in vivo study will measure the durability and the scaffold (gel) interaction with the chondral tissue in the induced chondral defects in sheep.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DE BARROS, CAIO NUNES; MILUZZI YAMADA, ANA LUCIA; JUNIOR, RUI SEABRA F.; BARRAVIERA, BENEDITO; HUSSNI, CARLOS ALBERTO; DE SOUZA, JAQUELINE BRANDAO; WATANABE, MARCOS JUN; RODRIGUES, CELSO ANTONIO; GARCIA ALVES, ANA LIZ. A new heterologous fibrin sealant as a scaffold to cartilage repair-Experimental study and preliminary results. Experimental Biology and Medicine, v. 241, n. 13, p. 1410-1415, JUL 2016. Web of Science Citations: 6.