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Reproductive performance and gonadal differentiation in piau-três-pintas, Leporinus friderici, Bloch 1794

Grant number: 12/14901-7
Support Opportunities:Scholarships in Brazil - Doctorate
Start date: October 01, 2012
End date: July 31, 2015
Field of knowledge:Agronomical Sciences - Fishery Resources and Fishery Engineering - Aquaculture
Principal Investigator:Sergio Ricardo Batlouni
Grantee:Thiago Gonçalves de Souza
Host Institution: Centro de Aquicultura (CAUNESP). Universidade Estadual Paulista (UNESP). Campus de Jaboticabal. Jaboticabal , SP, Brazil

Abstract

The main objective of this study is to evaluate the reproductive performance of wild L. friderici wild breeders induced with analogue gonadotropin releasing hormone and dopamine antagonist compound (ovopel ®) and carp pituitary extract (CE). Moreover, we aim to assess the suitability of using urea and / or tannin solutions , in order to remove the stickiness of the eggs. Finally, we want to determine the onset of gonads differentiation and the effect of incubation of the larvae at two different temperatures upon sexual proportion. To this end, 12 couples will be randomly distributed into two treatments (ovopel ® and CE) in six replicates. The female reproductive performance will be measured by the proportion of females that reach successful ovulation, the relative fecundity, fertilization and hatching rates, as well as oocyte diameter (mm). Concomitantly, a pool of eggs will be distributed into four treatments to remove egg stickiness in urea solution for 30, 60 and 90 min, and for 90 min. in urea solution followed by 10 minutes with vegetable tannin. Larvae from the best treatment will be kept in boxes (eight replicates), at 25 and 29 ° C for 90 days. After additional 30 days the sex ratiowill be estimated based on microscopic examination of the gonads, as well as the K condition factor, gonadosomatic index, growth rate and mortality. (AU)

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
DE SOUZA, THIAGO GONCALVES; KURADOMI, RAFAEL YUTAKA; RODRIGUES, SUELY MARLENE; BATLOUNI, SERGIO RICARDO. Wild Leporinus friderici induced spawning with different dose of mGnRHa and metoclopramide or carp pituitary extract. ANIMAL REPRODUCTION, v. 17, n. 1, p. 15-pg., . (12/14901-7)
THIAGO GONÇALVES DE SOUZA; RAFAEL YUTAKA KURADOMI; SUELY MARLENE RODRIGUES; SERGIO RICARDO BATLOUNI. Wild Leporinus friderici induced spawning with different dose of mGnRHa and metoclopramide or carp pituitary extract. Animal Reproduction, v. 17, n. 1, . (12/14901-7)
Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
SOUZA, Thiago Gonçalves de. Desempenho reprodutivo e diferenciação gonadal em piau-três-pintas, Leporinus friderici, Bloch 1794. 2015. Doctoral Thesis - Universidade Estadual Paulista (Unesp). Faculdade de Ciências Agrárias e Veterinárias. Jaboticabal Jaboticabal.