Activity of hepatic glucokinase, pyruvate kinase and fructose-1 ,6-bisphosphate in pacu (Piaractus mesopotamicus) fed different levels of protein and carbohydrate

• Grant number: 12/18117-9
• Support Opportunities: Scholarships abroad - Research Internship - Scientific Initiation
• Start date: December 01, 2012
• End date: January 31, 2013
• Field of knowledge: Agronomical Sciences - Animal Husbandry - Animal Nutrition and Feeding
• Principal Investigator: Leonardo Susumu Takahashi
• Grantee: Mayara de Moura Pereira
• Supervisor: Paula Enes
• Host Institution: Universidade Estadual Paulista (UNESP). Campus Experimental de Dracena. Dracena , SP, Brazil
• Institution abroad: Universidade do Porto (UP), Portugal
• Associated to the scholarship: 11/15187-3 - Energy metabolism in juvenile pacu fed diets containing different levels of protein and carbohydrates, BP.IC

Abstract

The proper utilization of dietary carbohydrates and the protein sparing effect result in decreased production costs and reduced nitrogen excretion. Therefore, studies are being conducted to analyze this possible sparing effect of dietary protein. Accordingly, the carbohydrate diet enables the dietary protein for maximum growth. The pacu (Piaractus mesopotamicus) represents an interesting biological model, as frugivore, allowing a better utilization of carbohydrates. Thus, will be studied the hepatic activity of glucokinase (GK), pyruvate kinase (PK) and fructose-1 ,6-bisphosphatase (FBPase) of pacu juveniles that were fed with six experimental diets (23/40, 19/45; 19/52, 21/38, 21/45 and 21/52% / PD% CHO). The results will be submitted to analysis of variance (ANOVA) and the means were compared by Duncan test (5%), through the SAS statistical software, version 9.0. (AU)