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Activity of hepatic glucokinase, pyruvate kinase and fructose-1 ,6-bisphosphate in pacu (Piaractus mesopotamicus) fed different levels of protein and carbohydrate

Grant number: 12/18117-9
Support type:Scholarships abroad - Research Internship - Scientific Initiation
Effective date (Start): December 01, 2012
Effective date (End): January 31, 2013
Field of knowledge:Agronomical Sciences - Animal Husbandry - Animal Nutrition and Feeding
Principal researcher:Leonardo Susumu Takahashi
Grantee:Mayara de Moura Pereira
Supervisor abroad: Paula Enes
Home Institution: Universidade Estadual Paulista (UNESP). Campus Experimental de Dracena. Dracena , SP, Brazil
Research place: Universidade do Porto (UP), Portugal  
Associated to the scholarship:11/15187-3 - Energy metabolism in juvenile pacu fed diets containing different levels of protein and carbohydrates, BP.IC


The proper utilization of dietary carbohydrates and the protein sparing effect result in decreased production costs and reduced nitrogen excretion. Therefore, studies are being conducted to analyze this possible sparing effect of dietary protein. Accordingly, the carbohydrate diet enables the dietary protein for maximum growth. The pacu (Piaractus mesopotamicus) represents an interesting biological model, as frugivore, allowing a better utilization of carbohydrates. Thus, will be studied the hepatic activity of glucokinase (GK), pyruvate kinase (PK) and fructose-1 ,6-bisphosphatase (FBPase) of pacu juveniles that were fed with six experimental diets (23/40, 19/45; 19/52, 21/38, 21/45 and 21/52% / PD% CHO). The results will be submitted to analysis of variance (ANOVA) and the means were compared by Duncan test (5%), through the SAS statistical software, version 9.0. (AU)

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