Study of markers for cell proliferation (Ki-67) and inflammation (COX-2) in uterus of bitches with cystic endometrial hyperplasia - pyometra

• Grant number: 12/20949-2
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: January 01, 2013
• End date: December 31, 2013
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Animal Reproduction
• Principal Investigator: Camila Infantosi Vannucchi
• Grantee: Ricardo Henrique Miziara
• Host Institution: Faculdade de Medicina Veterinária e Zootecnia (FMVZ). Universidade de São Paulo (USP). São Paulo , SP, Brazil

Abstract

The Cystic Endometrial Hyperplasia-Pyometra (CEH) complex is among the most common reproductive diseases in small animals, characterized by alterations in uterine tissues in different proportions and gradually, which are directly related to disease severity. The high prevalence and importance of CEH-Pyometra in bitches makes fundamental understanding of its pathophysiology. However, the pathophysiological mechanisms still represent a challenge for research related to reproductive disorders in dogs, since they are not fully elucidated. Thus, this study aims to characterize the proliferative activity of the endometrium and to determine the degree of inflammation of uterine tissues in dogs with CEH-Pyometra through marker Ki-67 and COX-2, respectively. Tissue samples will be collected from uterine bitches allocated into 2 groups, as follows: CEH-mucometra group - bitches with cystic endometrial hyperplasia-mucometra (n = 10) and Pyometra group - dogs with pyometra (n = 10). It is hoped that the results generated in this study may advance the knowledge about this uterine disease, to ensure bitches with better reproductive performance and contribute to the best therapy in clinical practice.