Gene expression profiling of human melanocyte cells after exposure to carbaryl and UV radiation

• Grant number: 12/23621-8
• Support Opportunities: Scholarships abroad - Research Internship - Doctorate
• Start date: September 23, 2013
• End date: March 22, 2014
• Field of knowledge: Biological Sciences - Pharmacology - Toxicology
• Principal Investigator: Silvia Berlanga de Moraes Barros
• Grantee: Bianca Ferrucio
• Supervisor: Richard S. Paules
• Host Institution: Faculdade de Ciências Farmacêuticas (FCF). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• Institution abroad: National Institutes of Health, Research Triangle Park (NIH), United States
• Associated to the scholarship: 10/17891-7 - Evaluation of the carbaryl influence on the effects of UV radiation in human melanocytes culture, BP.DR

Abstract

Toxicogenomics is rapidly evolving towards the discovery of useful biomarkers of toxicity, evaluation of multiple toxicity pathways, and elucidation of organisms' responses to environmental stress. Biological responses to environmental exposures can be very dynamic events, since a compound may have multiple modes of action, each with multiple key events. Therefore investigation of possible toxic effects is challenging, especially with regard to complex and long-term diseases such as cancer. Global gene expression analysis utilizing microarray technology is an efficient approach to determine altered pathways at the transcriptional level through simultaneously monitoring the expression of thousands of genes, and can be used for prediction and mechanistic assessment of human carcinogens at much lower doses and earlier times than traditional toxicity assays. In this research project, we aim to characterize carbaryl response profile in human melanocytes using microarrays and to search for genes possibly involved in carbaryl toxicity related to melanomagenesis, as well as comparing these responses to carbaryl exposure associated to UV radiation, to UV radiation alone and to non-treated melanoma cell lines. As it has been previously reported in an epidemiological study, we expect to be able to associate the carbaryl insecticide to cutaneous melanoma development; furthermore, we believe that this event may occur synergistically with UV radiation, the main etiologic factor for melanoma. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

FERRUCIO, BIANCA; TIAGO, MANOELA; FANNIN, RICHARD D.; LIU, LIWEN; GERRISH, KEVIN; MARIA-ENGLER, SILVYA STUCHI; PAULES, RICHARD S.; DE MORAES BARROS, SILVIA BERLANGA. Molecular effects of 1-naphthyl-methylcarbamate and solar radiation exposures on human melanocytes. TOXICOLOGY IN VITRO, v. 38, p. 67-76, FEB 2017. (10/17891-7, 12/23621-8, 11/12616-0)