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Gene expression profiling of human melanocyte cells after exposure to carbaryl and UV radiation

Grant number: 12/23621-8
Support type:Scholarships abroad - Research Internship - Doctorate
Effective date (Start): September 23, 2013
Effective date (End): March 22, 2014
Field of knowledge:Biological Sciences - Pharmacology - Toxicology
Principal researcher:Silvia Berlanga de Moraes Barros
Grantee:Bianca Ferrucio
Supervisor abroad: Richard S. Paules
Home Institution: Faculdade de Ciências Farmacêuticas (FCF). Universidade de São Paulo (USP). São Paulo , SP, Brazil
Research place: National Institutes of Health, Research Triangle Park (NIH), United States  
Associated to the scholarship:10/17891-7 - Evaluation of the carbaryl influence on the effects of UV radiation in human melanocytes culture, BP.DR


Toxicogenomics is rapidly evolving towards the discovery of useful biomarkers of toxicity, evaluation of multiple toxicity pathways, and elucidation of organisms' responses to environmental stress. Biological responses to environmental exposures can be very dynamic events, since a compound may have multiple modes of action, each with multiple key events. Therefore investigation of possible toxic effects is challenging, especially with regard to complex and long-term diseases such as cancer. Global gene expression analysis utilizing microarray technology is an efficient approach to determine altered pathways at the transcriptional level through simultaneously monitoring the expression of thousands of genes, and can be used for prediction and mechanistic assessment of human carcinogens at much lower doses and earlier times than traditional toxicity assays. In this research project, we aim to characterize carbaryl response profile in human melanocytes using microarrays and to search for genes possibly involved in carbaryl toxicity related to melanomagenesis, as well as comparing these responses to carbaryl exposure associated to UV radiation, to UV radiation alone and to non-treated melanoma cell lines. As it has been previously reported in an epidemiological study, we expect to be able to associate the carbaryl insecticide to cutaneous melanoma development; furthermore, we believe that this event may occur synergistically with UV radiation, the main etiologic factor for melanoma. (AU)

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
FERRUCIO, BIANCA; TIAGO, MANOELA; FANNIN, RICHARD D.; LIU, LIWEN; GERRISH, KEVIN; MARIA-ENGLER, SILVYA STUCHI; PAULES, RICHARD S.; DE MORAES BARROS, SILVIA BERLANGA. Molecular effects of 1-naphthyl-methylcarbamate and solar radiation exposures on human melanocytes. TOXICOLOGY IN VITRO, v. 38, p. 67-76, FEB 2017. Web of Science Citations: 1.

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