Influence of the incorporation of nanostructured silver vanadato in antimicrobial activity, morphology and mechanical properties of acrylic resins experimental.

• Grant number: 12/09347-0
• Support Opportunities: Scholarships in Brazil - Master
• Start date: March 01, 2013
• End date: August 31, 2014
• Field of knowledge: Health Sciences - Dentistry - Dental Materials
• Principal Investigator: Andréa Cândido dos Reis
• Grantee: Denise Tornavoi de Castro
• Host Institution: Faculdade de Odontologia de Ribeirão Preto (FORP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil

Abstract

This study aims to evaluate the in vitro antimicrobial activities and antibiofilme, morphology and mechanical performance acrylic resin embedded with nanostructured silver vanadate (VPn). The antimicrobial action of silver vanadate nanostructured be evaluated against the following species: Candida albicans (ATCC 1023), Streptococcus mutans (ATCC 25175), Staphylococcus aureus (ATCC 14990) and Pseudomonas aeruginosa (ATCC 27853), by determining the minimum inhibitory concentration (CIM). The biofilm is formed on the specimens of acrylic resins (incorporated and non-incorporated with nanostructured silver vanadate), under aerobic or microaerophilic incubation at 37 º C for 48h. The analysis antibiofilme be performed using the test methods of reducing the tetrazolium salt XTT-and confocal laser microscopy. The MIC is determined by the level of metabolic activity of viable cells according to the method of XTT immediately after the biofilm formation (48 h) and after 14 days. Determined these concentrations will be held the physicochemical characterization of modified materials by X-ray microanalysis (EDS) and scanning electron microscopy (SEM). The mechanical performance of the specimens with and without the addition (Control) of nanostructured silver vanadate (VPN) will be evaluated by bending tests, fracture resistance, surface hardness, surface roughness and impact resistance. The results will be statistically analyzed in Minitab software version 15.1.1.0, with normality and homogeneity tests for defining the use of parametric analysis or nonparametric.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DE CASTRO, DENISE T.; VALENTE, MARIANA L. C.; AGNELLI, JOSE AUGUSTO M.; DA SILVA, CLAUDIA H. LOVATO; WATANABE, EVANDRO; SIQUEIRA, RENATO L.; ALVES, OSWALDO L.; HOLTZ, RAPHAEL D.; DOS REIS, ANDREA C.. In vitro study of the antibacterial properties and impact strength of dental acrylic resins modified with a nanomaterial. JOURNAL OF PROSTHETIC DENTISTRY, v. 115, n. 2, p. 238-246, FEB 2016. (12/09124-1, 12/09347-0)

DE CASTRO, DENISE T.; VALENTE, MARIANA L. C.; DA SILVA, CLAUDIA H. L.; WATANABE, EVANDRO; SIQUEIRA, RENATO L.; SCHIAVON, MARCO A.; ALVES, OSWALDO LUIZ; DOS REIS, ANDREA C.. Evaluation of antibiofilm and mechanical properties of new nanocomposites based on acrylic resins and silver vanadate nanoparticles. ARCHIVES OF ORAL BIOLOGY, v. 67, p. 46-53, JUL 2016. (12/09124-1, 12/09347-0)

DENISE TORNAVOI DE CASTRO; RAPHAEL DIAS HOLTZ; OSWALDO LUIZ ALVES; EVANDRO WATANABE; MARIANA LIMA DA COSTA VALENTE; CLÁUDIA HELENA LOVATO DA SILVA; ANDRÉA CÂNDIDO DOS REIS. Development of a novel resin with antimicrobial properties for dental application. Journal of Applied Oral Science, v. 22, n. 5, p. 442-449, 2014-10-00. (12/09124-1, 12/09347-0)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

CASTRO, Denise Tornavoi de. Effect of the incorporation of nanostructured silver vanadate in antimicrobial activity, mechanical properties and morphology of acrylic resins. 2014. Master's Dissertation - Universidade de São Paulo (USP). Faculdade de Odontologia de Ribeirão Preto (PCARP/BC) Ribeirão Preto.